Regeneration of nerve crush injury using adipose‐derived stem cells: A multimodal comparison

Tremp, Mathias; Sprenger, Lima; Degrugillier, Lucas; Schaefer, Dirk J.; Madduri, Srinivas; Schaeren, Stefan; Kalbermatten, Daniel F.

doi:10.1002/mus.26188

Cited by 11 publications

(16 citation statements)

References 33 publications

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“… 50 However, significantly higher values in the treated groups point to the benefit of ASC and simple repair in preventing atrophy. 15 , 51 The SSI scores with early single-dose ASC treatment were superior to the results from crush studies with late administration (after 6 weeks), alluding to an immunomodulatory effect of ASC during early phases of neuroinflammation following PNI. 15 Such positive effects were confirmed on nerve histomorphometry, especially nerve fiber density, axon, and myelin area in the ASC-treated group.…”

Section: Discussionmentioning

confidence: 87%

“… 30 – 32 While the current body of knowledge supports both trans-differentiation to Schwann cell 33 or paracrine effects via neurotropic and neurotrophic factors 34 – 36 as possible mechanisms, a combination of both might hold true. 37 , 38 Most studies performed have explored efficacy of ASCs administered locally, 15 seeded in conduits, 8 , 14 , 17 , 39 , 40 or deposited in gels, 41 rather than incorporating ASC-derived neurospheres, exosomes, or conditioned media. 15 , 42 …”

Section: Discussionmentioning

confidence: 99%

“… 37 , 38 Most studies performed have explored efficacy of ASCs administered locally, 15 seeded in conduits, 8 , 14 , 17 , 39 , 40 or deposited in gels, 41 rather than incorporating ASC-derived neurospheres, exosomes, or conditioned media. 15 , 42 …”

Section: Discussionmentioning

confidence: 99%

“…Despite their value in reflecting the extent and quality of nerve regeneration, nerve histomorphometry, gastrocnemius weigh ratios, and electrophysiology suffer from poor correlation or lack functional/behavioral information. 14 , 15 Indeed, lack of tests combining objective functional motor evaluation with ease of repetitive testing without need for expensive setups is one of the chief limitations in nerve regeneration research.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Systemic Adipose-derived Stem Cell Therapy on Functional Nerve Regeneration in a Rodent Model

Schweizer

Schnider

Fanzio

et al. 2020

Plastic and Reconstructive Surgery - Global Open

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Background: Regardless of etiology, peripheral nerve injuries (PNI) result in disruption/loss of neuromuscular junctions, target muscle denervation, and poor sensorimotor outcomes with associated pain and disability. Adipose-derived stem cells (ASCs) have shown promise in neuroregeneration. However, there is a paucity of objective assessments reflective of functional neuroregeneration in experimental PNI. Here, we use a multimodal, static, and dynamic approach to evaluate functional outcomes after ASC therapy in a rodent PNI model. Methods: Lewis rats were divided into 3 groups: 10 mm sciatic nerve resection (“CUT” group; n = 10), transection and repair (“REP” group; n = 10), transection and repair plus single-dose ASCs (“ASC” group; n = 12). Allogeneic (Brown Norway rat) ASCs (1 × 106) were administered intravenously on postoperative day 1. Functional outcome was assessed by static sciatic index, toe spread factor, and a dynamic swim test on a weekly basis for 6 weeks. Sciatic nerves and gastrocnemius muscles were harvested at endpoint (6 weeks) for histological analysis. Results: The ASC group showed accelerated functional recovery on the swim test at 2 weeks postoperatively, with continued improvement over 4 weeks, culminating in superior overall outcomes at 6 weeks compared with the REP group. The CUT group showed no significant improvement from baseline. Nerve histomorphometry correlated well with the swim test results in the ASC group. Gastrocnemius muscle weights showed no difference between the REP and the ASC groups. Conclusion: Our study confirms that early, single dose, systemic administration of ASC after PNI accelerates and enhances overall motor recovery on static and dynamic functional tests as evidenced by improvements in voluntary as well as involuntary motions.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effect of Systemic Adipose-derived Stem Cell Therapy on Functional Nerve Regeneration in a Rodent Model

Schweizer

Schnider

Fanzio

et al. 2020

Plastic and Reconstructive Surgery - Global Open

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“…Stem cell transplantation is a very successful treatment modality using tissue engineering in many different diseases. The last two decades of studies have shown the positive effects of various stem cell therapies related to nerve regeneration, in both the central and peripheral nervous system [2,9,10]. It has been shown that the stem cell is differentiated into Schwann cell phenotype and accelerates axonal regeneration and provides better remyelinization [11,12].…”

Section: Introductionmentioning

confidence: 99%

IMPACT OF FETAL BRAIN TISSUE DERIVED MESENCHYMAL STEM CELL and FIBRIN GLUE ON FACIAL NERVE CRASH INJURY

et al. 2021

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Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury Background/aim: To evaluate the clinical and histopathological effects of fetal brain tissue derived mesenchymal stem cells(FBTMSC) and fibrin glue(FG) on the facial nerve(FN) regeneration in rats with traumatic FN injury. Materials and methods: 28 Sprague Dawley rats were included in the study and divided into 4 groups. Traumatic FN injury(FP) was created by a surgical clamp compression to the main trunk of left FN in all groups. In the control group(Group 1) no treatment was applied, in Group 2(FBTMSC group)2x10 6 FBTMSC was injected, in Group 3(FG group) only FG was applied, in group 4(FBTMSC & FG group) both FBTMSC and FG was applied to the injured section of the nerve. The FN functions were evaluated clinically, immediately after the procedure and at 3 rd-5 th-8 th weeks postoperatively. The FNs of all subjects were excised after the 8 th week; then the rats were sacrificed. The presence of stem cells in the injured zone was assessed by using BrdU(Bromo-deoxyuridine), and apoptosis was determined by TUNEL method. Results: After the damage, total FP was observed in all subjects. Statistically significant functional improvement was observed in Group 4 compared to all other groups(p<0.005). TUNEL positive cell count was statistically significantly higher in the control group than the other groups(p<0.001). TUNEL positive cell count was statistically significantly lower in Group 4 than the other groups. The proportion of BrdU-stained cells in Group 4(5%) was higher than Group 2(2%). 2 Conclusion: Clinically and histopathologically FBTMSC applied with FG may play a promising role as a regenerative treatment in posttraumatic FP.

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Adipose tissue stem cells in peripheral nerve regeneration—In vitro and in vivo

Rhode

Beier

Ruhl

2020

J of Neuroscience Research

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Regeneration of nerve crush injury using adipose‐derived stem cells: A multimodal comparison

Abstract: Animals treated with CR and epineural stem cell injection showed enhanced anatomical and functional recovery. Muscle Nerve 58: 566-572, 2018.

Cited by 11 publications

References 33 publications

Effect of Systemic Adipose-derived Stem Cell Therapy on Functional Nerve Regeneration in a Rodent Model

Effect of Systemic Adipose-derived Stem Cell Therapy on Functional Nerve Regeneration in a Rodent Model

IMPACT OF FETAL BRAIN TISSUE DERIVED MESENCHYMAL STEM CELL and FIBRIN GLUE ON FACIAL NERVE CRASH INJURY

Adipose tissue stem cells in peripheral nerve regeneration—In vitro and in vivo

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