Regeneration of Plants by Embryogenesis with Callus Cultures of Carum carvi L.

Furmanowa, M.; Olȩdzka, H.; Sowińska, Dorota

doi:10.1016/s0176-1617(84)80122-4

Cited by 9 publications

(2 citation statements)

References 5 publications

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“…As shown in the experiments with hypocotyl cultures in this study and with anther cultures (Smýkalová et al 2009), low temperature can be used for both induction processes, somatic and microspore embryogenesis in caraway. In caraway, somatic embryogenesis in suspension culture was induced by 3-indolebutyric acid (Furmanova et al 1984), and embryo development was affected by abscisic acid (Ammirato 1993). Our induction experiments were performed at 6°C and 30°C suggesting regeneration of plants regardless of the applied stress factor.…”

Section: Discussionmentioning

confidence: 99%

Induction conditions for somatic and microspore-derived structures and detection of haploid status by isozyme analysis in anther culture of caraway (Carum carvi L.)

Smýkalová

Horáček

Kubošiová

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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Plant regeneration was obtained from cultured anthers and hypocotyl segments of caraway (Carum carvi L.). Microspore-and somatic tissue-derived embryos were compared by observation of the regeneration process under identical induction conditions. Fluorescent microscopy with DAPI staining showed initiation of cell divisions and formation of embryogenic callus and somatic embryos from anther sacs, with production of embryos of both microspore and somatic origin. Induction of somatic embryos from hypocotyl-derived callus was also demonstrated. Isozyme native polyacrylamide gel electrophoresis was used to identify haploids and doubled haploids, and to determine the frequency of spontaneous diploidization of regenerated plants of microspore origin. Donor plants (2n= 20) and their anther-derived derivative plants (n=10, 2n= 20, 4n=40) in callus stage or leafy rosette stage were compared. The esterase (EST) band patterns of regenerated plants differed from the heterozygous parental material, suggesting that the regenerated plants were microsporederived haploid/doubled haploid plants. The similar profile of EST bands between the diploid anther-derived plants and a sample of the donor plants corresponded to a somatic regeneration pathway. Although the selected induction conditions revealed no preference for induction of microspore embryogenesis, the anther culture protocol established for caraway utilizing isozyme segregating EST loci markers is suitable for DH production.

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Section: Discussionmentioning

confidence: 99%

Induction conditions for somatic and microspore-derived structures and detection of haploid status by isozyme analysis in anther culture of caraway (Carum carvi L.)

Smýkalová

Horáček

Kubošiová

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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“…La capacitC de regheration in vitro, par la voie de I'embryogenhe somatique, est bien connue pour la famille des OmbelliRres (Ammirato 1983;Williams et Maheswaran 1986). A partir d'explants primaires, des plantes fonctionnelles fertiles ont Ct C obtenues pour le Daucus carota (Steward et al 1958), 1'Apium graveolens (Al-Abta et Collin 1978a), le Carum carvi (Furmanova et al 1984), le Foeniculum vulgare (Desmarest et al 1987) et le Petroselinum hortense (Watin et Bigot 1989).…”

Section: Introductionunclassified

Embryogenèse somatique et regénération de plantes à partir de protoplastes issus de suspensions cellulaires de céleri (Apium graveolens)

Watin-de-Pontfarcy¹,

Bigot²

1991

Can. J. Bot.

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Protoplasts were isolated from cell suspensions of Apium graveolens L. variety rapaceum (celeriac) initiated and subcultured on Gamborg medium (B5) with 2,4-dichlorophenoxyacetic acid (2.26 μM) and 6-benzylaminopurine (1.1 μM). The microcalli fraction above 450 μm was taken from 6-days-old cell suspensions and put into an enzyme mixture of cellulase Onozuka R-10 (2%) and pectolyase Y-23 (0.1%). Protoplasts were cultured on Lazar et al. modified medium with 2,4-dichlorophenoxyacetic acid (2.26 μM), naphthaleneacetic acid (0.89 μM), and zeatin (0.5 μM) at a density of 3 × 105∙mL−1. They were kept in darkness at alternating temperatures: 28 and 22 °C during 16 and 8 h, respectively. The culture medium was partially renewed after 7, 14, and 21 days of culture. Cell division rate represented about 30–40% of viable protoplasts. Addition of 2,4-dichlorophenoxyacetic acid (2.26 μM) in the microcalli plating medium improved embryogenic differentiation and subsequent plantlet isolation. Calli from protoplasts showed a variable capacity for somatic embryogenesis on Murashige and Skoog medium containing kinetin (1.4 μM). Plantlet growth was observed more particularly on microcalli plating medium without growth regulators. After acclimatization, a great number of plants with abnormal morphology was noted from calli cultivated on medium with kinetin. No variation of the normal ploidy level (2n = 2x = 22) has been recorded among the examined plants. Key words: Apium graveolens, protoplast, somatic embryogenesis, somaclonal variation, flowering.

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