Regeneration of Protochlorophyll in Etiolated Barley Seedlings Following Different Light Treatments

Augustinussen, E.; Madsen, Axel

doi:10.1111/j.1399-3054.1965.tb06941.x

Cited by 32 publications

(23 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The classical experiments on lag elimination in chlorophyll synthesis in higher plants (3,32,44,45,49), including the action spectrum determinations of Virgin (46), established the following: (a) the optimal length of dark period following preillumination is approximately 4 hr, which is about the length of the normal lag period in these organisms; (b) the saturation duration for preillumination is a few minutes at most; and (c) preillumination by blue light is ineffective in lag elimination. Effectiveness was concentrated in a single broad peak in the red-orange region of the spectrum, suggesting the participation of the red-far red system, although the reversal by far red light was ambiguous in the case of wheat (46).…”

Section: Discussionmentioning

confidence: 99%

“…The ability to convert protochlorophyll(ide) to chlorophyll(ide) in the dark has apparently been lost more slowly but is completely absent in the angiosperms, which are dependent on light for chlorophyll and chloroplast development. In these organisms, however, the concomitant elaboration of the phytochrome system has apparently resulted in this system's becoming an additional and often overriding control point in the induction of chloroplast formation (3,28,29,32,45,46,49), with the light-induced protochlorophyll(ide) to chlorophyll(ide) conversion assuming a secondary position. In Euglena, of course, the phytochrome system appears not to have been evolved, and the induction of chlorophyll and chloroplast formation is largely dependent on the light-induced conversion of protochlorophyll(ide) to chlorophyll(ide).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Events Surrounding the Early Development of Euglena Chloroplasts

Holowinsky¹,

Schiff

1970

Plant Physiol.

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Events Surrounding the Early Development of Euglena Chloroplasts

Holowinsky¹,

Schiff

1970

Plant Physiol.

View full text Add to dashboard Cite

“…Shibata (4) anid Btltler (5) nioted a lag phase in the dark synthesis which wN-as dependent oni the age of the leaf. Madsen (6) and Atgngstinnssen and Mladsen (7) reported a lag phase in the resynthesis.…”

mentioning

confidence: 99%

Protochlorophyllide Resynthesis in Dark-Grown Bean Leaves

Akoyunoglou

Siegelman

1968

Plant Physiol.

View full text Add to dashboard Cite

Abstract. The protochlorophyllide content of dark-grown bean leaves was determined at various ages. It was detectable the second day after germination and reached a maximum on abouit the tenth day.The resynthesis of protochlorophyllide following a single irradiation and the rate of the 685 to 673 m, shift of the newly formed chlorophyll was examined. Leaves up to 5-days old show no lag phase in protochilorophyllide resynthesis. By the sixth day, a lag phase is evident which increases with age. The rate of the 685 to 673 my shift is similarly age dependent.In the leaf, a dark synthesis of protochlorophyllide follows the lhototransformation of the pigment to chlorophyll. The time coutrse of the process was examine(d by several workers. Liro (1), Scharfniagel (2), alnd Virgin (3) found( no lag phase in the dark synithesis. Shibata (4) anid Btltler (5) nioted a lag phase in the dark synthesis which wN-as dependent oni the age of the leaf. Madsen (6) and Atgngstinnssen and Mladsen (7) reported a lag phase in the resynthesis.A systematic examination of the time couirse of protochlorophylli'de resynthesis followilng illtiminatioIn anfl -the spectral shifts of newly forme(l chlorophyll were made on (lark-grown beani leaves. The age of the tisstle has a profoinnd ilnflulenlce oIn many aspects of protochlorophyllide and the newly formetd chlorophyll, and these resullts are presented here. Materials and MethodsBeanl plants, Plh ResultsThe protochlorophrll ide cointeint of ,dark-grown bean leaves of variotus ages wNas examined. The pigment conteint is low in very youIIIg 'leaves, inlcreases rapidly to a maxiimal valtue and then remaiins constant (fig 1). The dark resynthesis of proto-T_

show abstract

“…If the increase produced by red light can be shown to be prevented by immediate far-red light these fatty acid changes could form part of the phytochrome-mediated elimination of the lag phase in chlorophyll formation (e.g. Augustinussen and Madsen 1965).…”

Section: Rfmentioning

confidence: 99%

Growth Inhibitors From Etiolated Leaves of Barley (Hordeum Vulgare L.)

Menhenett¹,

Carr²

1973

Aust. Jnl. Of Bio. Sci.

View full text Add to dashboard Cite

The unrolling of etiolated barley leaves, H. vulgare cv. Pallas, is phytochromemediated. After brief illumination with red light unrolling begins after 8 hr. The possibility that illumination results in the fall in content of an endogenous inhibitor led to the work reported.Inhibitory activity in an "acidic fraction" from etiolated barley leaves, as measured by leaf unrolling and wheat seed germination bioassays, declined in the 6-hr period in the dark following exposure to red light for 10 min.No abscisic acid (ABA) could be detected, either by paper chromatography and wheat coleoptile bioassay or by gas chromatography, in the inhibitory acidic fractions. The inhibitor is therefore not ABA. The inhibitory region on paper chromatograms did not exhibit a strong phenol-like reaction.Linoleic and linolenic acids, identified by gas chromatography and mass spectroscopy, were present in a 1 : 1 ratio in acidic fractions and linoleic acid inhibited unrolling. These fatty acids are chromatographically separable from the leaf-unrolling inhibitor(s). As they also increased nearly threefold in the leaves in the 6 hr following exposure to red light, they are not of interest to the present investigation. They possibly originate from etioplasts.

show abstract

Regeneration of Protochlorophyll in Etiolated Barley Seedlings Following Different Light Treatments

Cited by 32 publications

References 10 publications

Events Surrounding the Early Development of Euglena Chloroplasts

Events Surrounding the Early Development of Euglena Chloroplasts

Protochlorophyllide Resynthesis in Dark-Grown Bean Leaves

Growth Inhibitors From Etiolated Leaves of Barley (Hordeum Vulgare L.)

Contact Info

Product

Resources

About