Regeneration of renal proximal tubules after mercuric chloride injury is accompanied by increased binding of aminoacyl-transfer ribonucleic acid

Abstract

Homogenates of rat kidney cortex obtained 1,3 or 14 days after a single injection of HgCl2 were used to prepare the post-microsomal pH5 supernatant fraction. The activity of this fraction for peptide synthesis from [14C]phenylalanyl-tRNA was significantly increased at 1 and 3 days, at which time the proximal tubules are regenerating [Cuppage & Tate (1967) Am. J. Pathol. 51, 405-429]. This increased activity could not be attributed to a decreased inhibitory activity, but was due to an increased aminoacyl-tRNA binding, i.e. elongation-factor-1 activity, in the supernatant fraction.

Proteomics ofS-(1, 2-dichlorovinyl)-l-cysteine-induced acute renal failure and autoprotection in mice

“…This proposed mechanism is supported by the immunohistochemical finding of sustained presence of cytoplasmic and nuclear PSA-1 in the tubular epithelial cells in the autoprotected mice. Protein translation elongation factor is known to increase protein synthesis during repopulation of proximal tubules after injury by a single injection of HgCl 2 (38). In our study, we found that in lowdose and autoprotected groups, translation elongation factor was moderately downregulated, whereas with a lethal dose alone it was substantially underexpressed (Table 3), suggesting inhibition of essential protein synthesis needed for cell division and tubular repopulation of epithelial cells following high dose alone-induced injury.…”