Regeneration of soapnut tree through somatic embryogenesis and assessment of genetic fidelity through ISSR and RAPD markers

Singh, R. B.; Kashyap, Sandeep; Kumari, Nishi; Singh, Major

doi:10.1007/s12298-016-0364-0

Cited by 21 publications

(9 citation statements)

References 37 publications

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“…and Gonococci spp. using a disc diffusion method [ 34 ]. Pure young cultures of bacteria were obtained by subculturing the bacteria on agar-solidified Luria broth (LB) medium.…”

Section: Methodsmentioning

confidence: 99%

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Green Biosynthesis of Silver Nanoparticles Using Leaf Extract of Carissa carandas L. and Their Antioxidant and Antimicrobial Activity against Human Pathogenic Bacteria

et al. 2021

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Carissa carandas L. is traditionally used as antibacterial medicine and accumulates many antioxidant phytochemicals. Here, we expand this traditional usage with the green biosynthesis of silver nanoparticles (AgNPs) achieved using a Carissa carandas L. leaf extract as a reducing and capping agent. The green synthesis of AgNPs reaction was carried out using 1mM silver nitrate and leaf extract. The effect of temperature on the synthesis of AgNPs was examined using room temperature (25 °C) and 60 °C. The silver nanoparticles were formed in one hour by stirring at room temperature. In this case, a yellowish brown colour was developed. The successful formation of silver nanoparticles was confirmed by UV–Vis, Fourier transform infrared (FT-IR) and X-ray diffraction (XRD) analysis. The characteristic peaks of the UV-vis spectrum and XRD confirmed the synthesis of AgNPs. The biosynthesised AgNPs showed potential antioxidant activity through DPPH assay. These AgNPs also exhibited potential antibacterial activity against human pathogenic bacteria. The results were compared with the antioxidant and antibacterial activities of the plant extract, and clearly suggest that the green biosynthesized AgNPs can constitute an effective antioxidant and antibacterial agent.

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“…and Gonococci spp. using a disc diffusion method [ 34 ]. Pure young cultures of bacteria were obtained by subculturing the bacteria on agar-solidified Luria broth (LB) medium.…”

Section: Methodsmentioning

confidence: 99%

“…The MIC was calculated in a similar way as earlier reported [ 32 , 33 , 34 , 35 , 36 ]. The MIC of AgNPs (synthesized at 25 °C) was determined using a broth microdilution method.…”

Section: Methodsmentioning

confidence: 99%

Green Biosynthesis of Silver Nanoparticles Using Leaf Extract of Carissa carandas L. and Their Antioxidant and Antimicrobial Activity against Human Pathogenic Bacteria

et al. 2021

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“…ISSR primers have also been widely used in genetic stability assessment of Psidium guajava (Rai et al 2012b), Jatropha curcas (Mendel Soares et al 2016), including few Cucurbits such as Luffa acutangula (Velivela et al 2016), and Citrullus lanatus (Vinoth and Ravindhran 2016), owing to its higher reproducibility, more informative nature, no requirement of prior sequence information and freeness from radioactivity hazards (Phulwaria et al 2014). Use of more than one type of marker has always been recommended (Rathore et al 2014a, b;Saha et al 2015;Singh et al 2016) for a better analysis of genetic integrity, as different markers targets different regions in the genome (Venkatachalam et al 2007). Therefore, we also used two types of markers (RAPD and ISSR) in our study.…”

Section: Assessment Of Genetic Homogeneity In Micropropagated Plantsmentioning

confidence: 99%

An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop

Choudhary

Patel

Harish

et al. 2017

Physiol Mol Biol Plants

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Roxb. ex Willd., is a perennial and dioecious (2n = 28) plant of family Cucurbitaceae. Conventional methods of propagation through seeds, stem cuttings and rhizomatous/tuberous roots are inadequate for its mass cultivation as a vegetable crop. This paper reports an improved and efficient micropropagation method for wild female using nodal explants. Shoot amplification was achieved using subculturing of in vitro raised shoots on MS medium supplemented with various concentrations of 6-benzylaminopurine (BAP) alone or in combination with indole-3-acetic acid (IAA). The maximum number of shoots (45.30 ± 3.83) with an average length 6.52 ± 0.89 cm were differentiated on MS medium containing 0.5 mg L BAP, 0.1 mg L IAA and additives (50 mg L ascorbic acid, 25 mg L each of adenine sulphate, citric acid and l-arginine). The cloned shoots were rooted ex vitro. Each shoot treated with 250 mg L IBA for 5 min produced 12.3 ± 1.33 with a mean length 5.4 ± 0.73 cm. More than 85% (46 plants) of ex vitro rooted plantlets were successfully hardened in a greenhouse with normal growth characteristics. In order to evaluate the genetic stability of micropropagated plants, the two PCR-based techniques, Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) were used. The amplification patterns of the micropropagated and mother plant were monomorphic thus depicting genetic stability of the micropropagation system. This protocol could be effectively employed for the mass multiplication of wild female , a popular summer vegetable crop.

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“…The induction ratio and differentiation frequency of somatic embryos also vary with concentration ratio of cytokinin and auxin in Sapindus mukorossi Gaertn. [38], Eleusine coracana [39], and Coriandrum sativum [40]. Conclusively, BA (as a kind of cytokinin) and NAA (as a kind of auxin) were effective for somatic embryo induction from embryo, cotyledon, and hypocotyl explants of P. ostii 'Fengdan' and most appropriate concentration were 3.0 mg·L −1 and 1.0 mg·L −1 , respectively.…”

Section: Discussionmentioning

confidence: 86%

Enhanced Somatic Embryo Induction of a Tree Peony, Paeonia ostii ‘Fengdan’, by a Combination of 6-benzylaminopurine (BA) and 1-naphthylacetic Acid (NAA)

Ren

Jeong

2019

Plants

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Somatic embryogenesis is a preferred method for vegetative propagation due to its high propagation efficiency. In this study, zygotic embryos, cotyledons, and hypocotyls of Paeonia ostii ‘Fengdan’ were used as the explant to induce somatic embryogenesis. The results showed that a combination of 0.5 mg·L−1 thidiazuron (TDZ) and 0.5 mg·L−1 2,4-dichlorophenoxyacetic acid (2,4-D) was effective in inducing somatic embryos from the zygotic embryo and cotyledon explants. Hypocotyls only formed somatic embryos on Murashige and Skoog (MS) medium supplemented with both 0.5 mg·L−1 TDZ and 0.5 mg·L−1 1-naphthylacetic acid (NAA). Moreover, the compact callus was effectively produced from zygotic embryo, cotyledon, and hypocotyl explants in medium supplemented with a combination of 3.0 mg·L−1 6-benzylaminopurine (BA) and 1.0 mg·L−1 NAA, and then converted into somatic embryos in the same medium, and the ratio of the explants with embryo induction and number of embryos induced per explant were much higher than those induced by 0.5 mg·L−1 TDZ and either 0.5 mg·L−1 2,4-D or 0.5 mg·L−1 NAA. The MS medium was better than the woody plant medium (WPM) for inducing somatic embryos from zygotic embryo and hypocotyl explants, whereas the WPM was better than the MS medium for somatic embryogenesis induction from cotyledon explants. All of the somatic embryos developed well into mature embryos on their respective media supplemented with both 3.0 mg·L−1 BA and 1.0 mg·L−1 NAA. Overall, the protocols for indirect somatic embryogenesis from zygotic embryo, cotyledon, and hypocotyl of P. ostii ‘Fengdan’ were successfully established, which can greatly facilitate their propagation and breeding processes.

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Regeneration of soapnut tree through somatic embryogenesis and assessment of genetic fidelity through ISSR and RAPD markers

Cited by 21 publications

References 37 publications

Green Biosynthesis of Silver Nanoparticles Using Leaf Extract of Carissa carandas L. and Their Antioxidant and Antimicrobial Activity against Human Pathogenic Bacteria

Green Biosynthesis of Silver Nanoparticles Using Leaf Extract of Carissa carandas L. and Their Antioxidant and Antimicrobial Activity against Human Pathogenic Bacteria

An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop

Enhanced Somatic Embryo Induction of a Tree Peony, Paeonia ostii ‘Fengdan’, by a Combination of 6-benzylaminopurine (BA) and 1-naphthylacetic Acid (NAA)

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