Regeneration of somatic embryos of oil palm (Elaeis guineensis) using temporary immersion bioreactors

Gomes, Hugo Teixeira; Bartos, Patrícia Monah Cunha; Balzon, Talita Aparecida; Scherwinski‐Pereira, Jonny Everson

doi:10.1016/j.indcrop.2016.05.021

Cited by 34 publications

(31 citation statements)

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“…However, the species's somatic embryogenesis is insufficiently known and understood. To date, protocols developed for the crop still provide several limitations, such as the formation of calli with low morphogenic potential, lack of synchronization in embryogenic development, low conversion rate of somatic embryos and regeneration of incomplete plants (Konan et al 2010;Soh et al 2011;Silva et al 2012;Thuzar et al 2012;Gomes et al 2015Gomes et al , 2016.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of morphological and anatomical changes in leaf tissues of an interspecific hybrid of oil palm during acquisition and development of somatic embryogenesis

Gomes

Bartos

Scherwinski‐Pereira

2017

Plant Cell Tiss Organ Cult

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420 days of cultivation, the propagules formed somatic embryos with no connection to source tissues, initially composed (globular stage) of a very characteristic ground meristem and protoderm. After 480 days of cultivation, as the cultures matured (torpedo stage), procambial strands, a structural characteristic also observed in mature zygotic embryos, were reported. The results provide an in-depth understanding of somatic embryogenesis of immature leaves of oil palm. Further, current analysis develops morphological markers at different stages of development obtained during the process.

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Section: Introductionmentioning

confidence: 99%

Dynamics of morphological and anatomical changes in leaf tissues of an interspecific hybrid of oil palm during acquisition and development of somatic embryogenesis

Gomes

Bartos

Scherwinski‐Pereira

2017

Plant Cell Tiss Organ Cult

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“…Somatic embryogenesis and organogenesis are the two main modes of micropropagation of date palm, which are widely accepted throughout the tissue culture arena in the world (Bhansali, 2010). The utilization of bioreactors is also making a significant contribution toward micropropagation of different plant species (Frómeta et al, 2017;Gomes et al, 2016;Othmani et al, 2011).…”

Section: Micropropagationmentioning

confidence: 99%

“…The Temporary Immersion Bioreactor (TIB) showed that shoot clusters were regenerated 5.5-fold faster as compared to semisolid medium (Othmani et al, 2011). The bioreactor system may be used in the micropropagation optimization process, which needs less manipulation of medium, reduces the cost of gelling agents, experienced labor, laboratory space and induces the multiplication frequency of the particular plant species (Gomes et al, 2016). Recently, Frómeta et al (2017) successfully achieved the regeneration of the Barberton daisy (Gerbera jamesonii) using TIB system.…”

Section: Bioreactormentioning

confidence: 99%

“…The high number of shoots observed in the TIB followed by RITA and GIB and optimum rooting was found in TIB compared to GIB and RITA. Gomes et al (2016) evaluated the regeneration of somatic embryos of oil palm (Elaeis guineensis) by using semisolid, RITA and Temporary Immersion System (TIS) and found TIS was most suitable for regeneration. Whereas, the use of TIB system in date palm (cv.…”

Section: Bioreactormentioning

confidence: 99%

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Date palm micropropagation: Advances and applications

Al-Khayri

Naik

2017

Ciênc. agrotec.

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Date palm (Phoenix dactylifera L.) is a fruit tree resilient to adverse climatic conditions predominating in hot arid regions of the Middle East and North Africa. The date fruit contains numerous chemical components that possess high nutritional and medicinal values. Traditional propagation by offshoots is inefficient to satisfy current demands for date palm trees. Alternatively, micropropagation provides an efficient means for large-scale propagation of date palm cultivars. Both somatic embryogenesis and organogenesis, either directly or indirectly though the callus phase, have been demonstrated in date palm in vitro regeneration. Culture initiation commonly utilizes shoot-tip explants isolated from young offshoots. Recently, the immature inflorescences of adult trees were utilized as an alternative nondestructive source of explants. In addition to the nature of the explant used, successful plant regeneration depends on the cultivar, composition of the culture medium and physical status. Challenges of date palm micropropagation include long in vitro cycle, latent contamination, browning, somaclonal variation as well as ex vitro acclimatization and transplanting. A remarkable amount of research investigating these factors has led to optimized protocols for the micropropagation of numerous commercially important cultivars. This has encouraged the development of several international commercial tissue culture laboratories. Molecular characterization provides an assurance of genetic conformity of regenerated plantlets, a key feature for commercial production. This article describes date palm micropropagation protocols and also discusses recent achievements with respect to somaclonal variation, molecular markers, cryopreservation and future prospects.Index terms: Cryopreservation; somatic embryogenesis; somaclonal variation; organogenesis; molecular marker. RESUMOA tamareira (Phoenix dactylifera L.) é uma arvore frutífera adaptada à condições climáticas adversas predominantemente em regiões áridas do Oriente Médio e Norte Africano. As tâmaras possuem vários componentes químicos com alto valor medicinal e nutricional. A propagação tradicional por estacas não é suficiente para satisfazer a demanda por mudas e assim, a micropropagação apresenta-se como uma alternativa eficiente para a produção de mudas em larga escala. Embriogênese somática e organogênese, tanto direta quanto indireta via calos, tem sido usada para obter a regeneração in vitro de tamareira. O inicio do cultivo in vitro normalmente utiliza meristemas excisados de brotações jovens. Recentemente, inflorescências imaturas de árvores adultas são usadas como fonte alternativa de explantes não destrutiva. Além da origem do explante, o sucesso da regenerção depende do cultivar, da composição do meio de cultura e de condições físicas. Desafios na micropropagação de tamareira incluem um longo ciclo in vitro, contaminação, escurecimento do tecido, variação somaclonal além do enraizamento e aclimatização ex vitro. Diversos estudos investigando esses fatore...

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“…Small isodiametric cells with large nuclei and prominent nucleoli, mitochondria, cytoplasm with little intercellular space, and a cell system organized in cells with embryogenic potential can be visualized through toluidine blue stain. Non-embryogenic calli mostly have large, elongated, and vacuolated cells, with intercellular spaces and a disorganized cell system, and they are not viable (Pádua et al, 2013;Gomes et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

HISTOLOGICAL ANALYSIS OF INDIRECT SOMATIC EMBRYOGENESIS INDUCED FROM ROOT EXPLATS OF OIL PALM (Elaeis guineensis Jacq)

et al. 2019

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Oil palm is economically important as a crop with high oil production. Indirect somatic embryogenesis in oil palm requires a long time for callus induction and plant formation, and it is important to study the embryogenic potential of calli and the mechanisms of somatic embryogenesis. The aim of this study was to test different growth regulators and spermine in induction of embryogenic calli in root explants of oil palm (Elaeis guineensis Jacq). Apex root explants of approximately 0.5 cm were isolated from plants cultivated in vitro and inoculated in Y3 culture medium in the following treatments: A - without growth regulators; B - 1 mg.L-1 picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid); C - 1 mg.L-1 picloram and 2 mg.L-1 2ip (2-isopentenyladenine); D - 2 mg.L-1 2ip; E - 1 mg.L-1 picloram and 2 mg.L-1 BAP (6-benzylaminopurine); F - 2 mg.L-1 BAP; and G - 14.5 mg.L-1 spermine. After six months of culturing, the calli induced in the treatments were analyzed by light microscopy. The calli induced in the treatments with 1 mg.L-1picloram (B) and treatment with 14.5 mg.L-1spermine (G) exhibited embryogenic characteristics, small and isodiametric cells, forming agglomerates, besides a large amount of starch. Calli of the best treatment (Y3 com 1 mg.L-1 de picloram) were inoculated in Y3 culture medium without addition of growth regulators. After eight months, calli were once more analyzed under light microscopy. All the treatments showed callus formation, except for treatments D and A. Calli of treatment B exhibited cells with embryogenic characteristics that developed somatic embryos.

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Regeneration of somatic embryos of oil palm (Elaeis guineensis) using temporary immersion bioreactors

Cited by 34 publications

References 31 publications

Dynamics of morphological and anatomical changes in leaf tissues of an interspecific hybrid of oil palm during acquisition and development of somatic embryogenesis

Dynamics of morphological and anatomical changes in leaf tissues of an interspecific hybrid of oil palm during acquisition and development of somatic embryogenesis

Date palm micropropagation: Advances and applications

HISTOLOGICAL ANALYSIS OF INDIRECT SOMATIC EMBRYOGENESIS INDUCED FROM ROOT EXPLATS OF OIL PALM (Elaeis guineensis Jacq)

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