Regeneration of the femoral epicondyle on calcium-binding silk scaffolds developed using transgenic silk fibroin produced by transgenic silkworm

Nagano, Aya; Tanioka, Yumi; Sakurai, Nobuko; Sezutsu, Hideki; Kuboyama, Noboru; Kiba, Hideo; Tanimoto, Yasuhiro; Nishiyama, Norihiro; Asakura, Tetsuo

doi:10.1016/j.actbio.2010.10.032

Cited by 34 publications

(23 citation statements)

References 40 publications

(74 reference statements)

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“…When 18 considering the significance of coating and base materials, the biological reaction to vascular grafts depends 19 more on the coating material than on the base material at an early stage after implantation. As proof of this, 20 organization and tissue infiltration was prominent among coating materials. anti-inflammatory effect were observed on SF/SF graft.…”

supporting

confidence: 56%

“…In addition, elastic modulus of that SF and PET graft coated by same material showed no significant 20 differences under compression test. According to this we believe SF base have ability to use as a vascular M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT graft.…”

mentioning

confidence: 78%

“…No significant differences were found in the 19 comparisons of graft bases. On the other hand, comparing the coating, compressive elastic modulus of20 gelatin was significantly lower than silk fibroin(Figure3(B)). .…”

mentioning

confidence: 93%

“…In the case of the SF base, the degumming process, which removes silk 5 sericin coated on the silk fibroin, was performed [16,20]. bases.…”

mentioning

confidence: 99%

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Biological Reaction to Small-Diameter Vascular Grafts Made of Silk Fibroin Implanted in the Abdominal Aortae of Rats

Fukayama

Takagi

Tanaka

et al. 2015

Annals of Vascular Surgery

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supporting

confidence: 56%

mentioning

confidence: 78%

mentioning

confidence: 93%

“…In the case of the SF base, the degumming process, which removes silk 5 sericin coated on the silk fibroin, was performed [16,20]. bases.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Biological Reaction to Small-Diameter Vascular Grafts Made of Silk Fibroin Implanted in the Abdominal Aortae of Rats

Fukayama

Takagi

Tanaka

et al. 2015

Annals of Vascular Surgery

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“…This phenomenon maybe due to the hydrophilic Ca-binding sites genetically introduced into the hydrophobic H-chain of SF. After implanting this transgenic SF-based scaffold into the femoral epicondyle in a rabbit model, early mineralization and bone formation were observed (Figure 4) [175]. Reprinted with permission from Nagano et al [175].…”

Section: Silk Fibroin Mineralizationmentioning

confidence: 99%

Silk as a potential candidate for bone tissue engineering

Mottaghitalab

Hosseinkhani

Shokrgozar

et al. 2015

Journal of Controlled Release

146

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Silk‐Based Biomaterials

Gomes¹,

Leonor²,

Mano³

et al. 2012

Biomimetic Approaches for Biomaterials Development

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IntroductionSilk is a hierarchically structured fibrous protein that has been used for thousands of years for different applications such as textile production and wound dressings [1]. Although silk proteins are produced by a large number of arthropod species such as wasps, bees, and crickets, during the past few decades, the scientific community has focused its attention in the silk produced by the mulberry silkworm Bombyx mori and by different spider species. Farming of the silkworm B. mori started around 5000 years ago in China, and since then, the cocoon case produced by this animal has been used as a source of silk for the textile industry. More recently, B. mori silk has been used commercially in the development of sutures for medical applications [1,2]. In contrast to B. mori silk, spider silk has not been commercialized for biomedical proposes mainly because of the territorial cannibalistic behavior of spiders, which makes them difficult to farm, along with the low levels of silk production. However, in the past, spider webs have been used as bandages for wound dressing, because of its antimicrobial properties, and in other human activities such as fishing and hunting [3]. The results from recent studies demonstrating the biocompatibility of silk and the exquisite mechanical properties of silk fibers, especially in the case of spider silk, are the main reasons why B. mori and spider silk proteins have been the target of intense research efforts, when compared with the silk produced by other arthropods, aimed at developing new silk-based biomaterials with superior mechanical and biological properties.On the basis of the background given above and since B. mori and spider silk are the most extensively characterized silk, this chapter focuses on the silk proteins produced by these animals and on the perspectives of using these proteins for future biomedical applications.The silk produced by B. mori is the most widely used, since this silkworm is easy to farm, allowing for steady and large-scale production [1]. The B. mori silk fiber is formed by two microfilaments embedded in a gluelike glycoprotein named sericin, which works as a coating and is used by the animal for cocoon assembly. Each microfilament results from the assembly of a hydrophobic ∼370 kDa heavy-chain fibroin protein, with a relatively hydrophilic ∼25 kDa light-chain fibroin and a 30 kDa P25 protein [4]. The heavy-chain fibroin is a large insoluble macromolecule formed by ∼5263 amino acids and is rich in glycine (G, 45.9%), alanine (A, 30.3%), serine (S, 12.2%), tyrosine (Y, 5.3%), and valine (V, 1.8%) residues [1,5]. This heavy-chain fibroin is formed by a highly repetitive crystalline fraction of 2377 repeats of GX. The X position is occupied by the A residue in 64% of the repeats, by S in 22%, by Y in 10%, by V in 3%, and by threonine (T) in 1.3% of the repeats [5,6]. These repetitive cores form 12 domains that alternate with 11 nonrepetitive amorphous domains formed by 25 amino acids residues, which connect the crystalline domains. These a...

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Regeneration of the femoral epicondyle on calcium-binding silk scaffolds developed using transgenic silk fibroin produced by transgenic silkworm

Cited by 34 publications

References 40 publications

Biological Reaction to Small-Diameter Vascular Grafts Made of Silk Fibroin Implanted in the Abdominal Aortae of Rats

Biological Reaction to Small-Diameter Vascular Grafts Made of Silk Fibroin Implanted in the Abdominal Aortae of Rats

Silk as a potential candidate for bone tissue engineering

Silk‐Based Biomaterials

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