Regeneration of transformed plants from hairy roots of Plumbago indica

Gangopadhyay, Moumita; Chakraborty, Debraj; Bhattacharyya, Sukanta; Bhattacharya, Subhajit

doi:10.1007/s11240-010-9702-z

Cited by 52 publications

(30 citation statements)

References 37 publications

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“…In our study no shoot induction was observed in the hormone-free medium which resembles the results of studies on Pogostemon cablin by He-Ping et al (2011). In contrast, spontaneous regeneration of transformed plants from hairy roots of Plumbago indica (Gangopadhyay et al 2010) and Centaurium erythraea (Subotic et al 2009) was reported. Mano and Matsuhashi (1995) reported that horseradish plants can easily induce adventitious shoots from hairy roots without the addition of plant growth regulators.…”

Section: Shoot Regeneration From Hairy Root Culturessupporting

confidence: 87%

Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi

Sharafi

Sohi

Azadi

et al. 2014

Physiol Mol Biol Plants

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An efficient hairy root induction system for an important endangered medicinal plant, Dracocephalum kotschyi, was developed through Agrobacterium rhizogenesmediated transformation by modifying the co-cultivation medium using five bacterial strains, A4, ATCC15834, LBA9402, MSU440, and A13 (MAFF-02-10266). A drastic increase in transformation frequency was observed when a Murashige and Skoog medium lacking NH 4 NO 3 KH 2 PO 4, KNO 3 and CaCl 2 was used, resulting in hairy root induction frequencies of 52.3 %, 69.6 %, 48.6 %, 89.0 %, and 80.0 % by A4, A13, LBA9402, MSU440, and ATCC15834 strains, respectively. For shoot induction, hairy roots and unorganized tumors induced by strain ATCC15834 were placed on an MS media supplemented with 0.1, 0.25, 0.5, and 1 mg/l BA plus 0.1 mg/l NAA. The high frequency of shoot regeneration and number of shoot were obtained in the medium containing 0.25 mg/l BA and 0.1 mg/l NAA. Root induction occurred from the base of regenerated shoots on the MS medium supplemented with 0.5 mg/l IBA after 10 days.

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Section: Shoot Regeneration From Hairy Root Culturessupporting

confidence: 87%

Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi

Sharafi

Sohi

Azadi

et al. 2014

Physiol Mol Biol Plants

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“…On the other hand, in our study, an addition of any plant growth regulators nor changing the culture conditions were not necessary for obtaining shoot regeneration from hairy roots. Spontaneous regeneration of shoots from hairy root cultures has been also reported earlier for, inter alia T. indica (Chaudhuri et al 2006), Centaurium erythraea (Piatczak and Wysokinska 2006), P. indica (Gangopadhyay et al 2010), Ophiorrhiza rugosa (Kamble et al 2011) and Rauwolfia serpentina (Mehrotra et al 2013). According to Mehrotra et al (2013) the depletion of nutrient contents during continuous growth and changes in levels or sensitivity to phytohormones in transformed tissues can be considered as factors responsible for the spontaneous regeneration of shoots.…”

Section: Discussionsupporting

confidence: 74%

“…Several differences were observed in the morphology and biomass of SD-and HR-R. glutinosa plants, most notably, a considerable increase in root biomass of the HR-plants. This is one of the most commonly observed properties of pRi-transformed regenerants, which was found also in such transgenic plants as Catharanthus roseus (Choi et al 2004), T. indica (Chaudhuri et al 2006), Aralia elata (Kang et al 2006) and P. indica (Gangopadhyay et al 2010). The increase is due to the influence of the introduced rol genes on hormonal synthesis and sensitivity.…”

Section: Discussionmentioning

confidence: 53%

“…According to Christey (2001) transgenic plants have been obtained after A. rhizogenes-mediated transformation of 89 different taxa, representing more than 79 plant species, and more transformed plant taxa, e.g. Plumbago indica (Gangopadhyay et al 2010) or Tylophora indica (Chaudhuri et al 2006) demonstrate higher level of secondary metabolites than untransformed plants.…”

mentioning

confidence: 99%

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Iridoid and phenylethanoid glycoside production and phenotypical changes in plants regenerated from hairy roots of Rehmannia glutinosa Libosch.

Piątczak

Kuźma

Skała

et al. 2015

Plant Cell Tiss Organ Cult

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Spontaneous shoot regeneration was obtained from the hairy roots of Rehmannia glutinosa cultured in liquid woody plant medium for 4 weeks in darkness after induction by Agrobacterium rhizogenes strain A4. These shoots were proliferated on solid Murashige and Skoog (MS) medium in 300 mL glass jars and in liquid MS medium in a 5 L nutrient sprinkle bioreactor. These media were supplemented with 0.1 mg L -1 of indole-3-acetic acid (IAA) and 1.0 mg L -1 of 6-benzylaminopurine. All shoots became rooted on MS agar medium containing IAA or indole-3-butyric acid within 4 weeks. The plantlets were then transferred into soil and grew in a greenhouse. The transgenic nature of the plants was confirmed by polymerase chain reaction (PCR) and reverse-transcriptase PCR analyses. The pRi-regenerated transformants demonstrated a few phenotypical alterations. Production of six iridoid (catalpol, aucubin, loganin, catalposide, harpagide, harpagoside) and two phenylethanoid (verbascoside and isoverbascoside) glycosides was determined in shoots cultured in vitro and leaves and roots of 6-and 12-month-old plants by ultra high performance liquid chromatography. The shoot culture was able to synthesize low amounts of catalpol, verbascoside and isoverbascoside. Leaves and roots of pRi-regenerants accumulated similar (harpagoside, isoverbascoside), higher (catalposide, aucubin, harpagide, verbascoside) or lower (catalpol, loganin) levels of the metabolites in comparison with untransformed R. glutinosa plants and their content varied with the age of the plants and the organ analyzed. However, the increase in shoot and root biomass of transformed plants resulted in a significantly greater yield of all analyzed compounds.

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“…Rapid growth, low doubling time, easy maintenance and the ability to synthesize a wide variety of chemical compounds, are some of the advantages that have made hairy roots as an important source for the production of secondary metabolites. Genetic transformation by Agrobacterium rhizogenes is an effective method to increase the accumulation of secondary metabolites in plant cells (Gangopadhyay et al 2010). A. rhizogenes is a gram-negative, soil bacterium.…”

Section: Introductionmentioning

confidence: 99%

A reliable and efficient protocol for induction of hairy roots in Agastache foeniculum

2014

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Hairy root culture system is a valuable tool to study the characteristics of gene expression, gene function, root biology, biochemical properties and biosynthesis pathways of secondary metabolites. In the present study, hairy roots were established in Anise hyssop (Agastache foeniculum) via Agrobacterium rhizogenes. Three strains of Agrobacterium rhizogenes (A4, A7 and 9435), were used for induction of hairy roots in four various explants (hypocotyl, cotyledon, onemonth-old leaf and five-month-old leaf) of Anise hyssop. The highest frequency of transformation was achieved using A4 strain in one-month-old leaves (51.1%). The transgenic states of hairy root lines were confirmed by PCR (Polymerase chain reaction) method. High performance liquid chromatography analysis revealed that the production of rosmarinic acid (RA) in transformed roots of A. foeniculum was almost 4-fold higher than that of the non-transformed roots. In a separate experiment, hairy roots obtained from one-month-old leaves inoculated with A4 strain, were grown in liquid medium and the effects of different concentrations of salicylic acid (0.0, 0.01, 0.1 and 1 mM) and chitosan (0, 50, 100 and 150 mg L −1 ) (as elicitor) and sucrose (20, 30, 40 and 50 g L −1 ) on the growth of hairy roots were evaluated. The results showed that, 30 g L −1 sucrose and 100 mg L −1 chitosan increased the biomass of hairy root cultures and application of salicylic acid reduced the growth of hairy roots compared with control roots.

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Regeneration of transformed plants from hairy roots of Plumbago indica

Cited by 52 publications

References 37 publications

Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi

Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi

Iridoid and phenylethanoid glycoside production and phenotypical changes in plants regenerated from hairy roots of Rehmannia glutinosa Libosch.

A reliable and efficient protocol for induction of hairy roots in Agastache foeniculum

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