Regeneration of Transgenic Oil Palm Carrying GFP Gene Used as a Visual Selectable Marker

Abstract

Green fluorescent protein (GFP)

VECTOR CONSTRUCTION AND TRANSIENT EVALUATION IN OIL PALM CALLI VIA Agrobacterium-MEDIATED TRANSFORMATION

“…This result indicated that the CaMV35S promoter was superior in driving the expression of gfp genes in oil palm calli. This result was in agreement with previous studies that reported that oil palm calli bombarded with a construct carrying gfp gene driven by the CaMV35S promoter had the highest number of GFP signals as compared with the Ubi-1 promoter (Majid and Parveez, 2007;2016;Parveez and Majid, 2008;. A recent study by Fizree et al (2019) using DsRED gene also showed that the CaMV35S promoter was the strongest promoter compared with other promoters.…”

“…In addition, genes encoding the red fluorescent protein (RFP), the DsRED and its improved version, DsRED2, were also used in plant studies (Lin et al, 2011). For oil palm transformation, the gusA (Masura et al, 2010;, gfp (Bahariah et al, 2017;Majid and Parveez, 2016;Parveez and Majid, 2008; and DsRED (Fizree et al, 2019), were used as the reporter genes.…”

“…The number of GFP signals was the highest in calli transformed with the pBAR65 vector, followed by pNH1, pBARSGFP, pBINUBG, pNH2 and pPZUBG (Figure 7). Previously, the use of gfp as a reporter gene in oil palm has been reported (Bahariah et al, 2017;Majid and Parveez, 2007;2016;Parveez and Majid, 2008;. The reports suggested that the use of gfp gene as a reporter gene could facilitate the detection of successful transformation because the selection and regeneration of transformed oil palm tissues are very time-consuming (Masani et al, 2018).…”