Regeneration of uterine horns in rats by collagen scaffolds loaded with collagen-binding human basic fibroblast growth factor

Li, Xin’an; Sun, Haixiang; Lin, Nacheng; Hou, Xun; Wang, Jingmei; Zhou, Bai; Xu, Pengju; Xiao, Zhifeng; Chen, Bing; Dai, Jianwu; Hu, Yali

doi:10.1016/j.biomaterials.2011.07.050

Cited by 142 publications

(84 citation statements)

References 30 publications

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“…(Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, PR China) as previously described [15][16][17][18]. The size of the collagen membranes was 5.0 cm×3.0 cm×0.2 cm, and the membranes were sterilized by 12kGy Co 60 irradiation.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model

Jia

Tang

et al. 2015

Biomaterials

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Section: Accepted Manuscriptmentioning

confidence: 99%

Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model

Jia

Tang

et al. 2015

Biomaterials

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“…Biological materials, such as collagen [18], for uterine tissue regeneration has been reported. While promising, the regeneration speed was considered slow.…”

Section: Introductionmentioning

confidence: 99%

Application of Detergents or High Hydrostatic Pressure as Decellularization Processes in Uterine Tissues and Their Subsequent Effects on In Vivo Uterine Regeneration in Murine Models

et al. 2014

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Infertility caused by ovarian or tubal problems can be treated using In Vitro Fertilization and Embryo Transfer (IVF-ET); however, this is not possible for women with uterine loss and malformations that require uterine reconstruction for the treatment of their infertility. In this study, we are the first to report the usefulness of decellularized matrices as a scaffold for uterine reconstruction. Uterine tissues were extracted from Sprague Dawley (SD) rats and decellularized using either sodium dodecyl sulfate (SDS) or high hydrostatic pressure (HHP) at optimized conditions. Histological staining and quantitative analysis showed that both SDS and HHP methods effectively removed cells from the tissues with, specifically, a significant reduction of DNA contents for HHP constructs. HHP constructs highly retained the collagen content, the main component of extracellular matrices in uterine tissue, compared to SDS constructs and had similar content levels of collagen to the native tissue. The mechanical strength of the HHP constructs was similar to that of the native tissue, while that of the SDS constructs was significantly elevated. Transmission electron microscopy (TEM) revealed no apparent denaturation of collagen fibers in the HHP constructs compared to the SDS constructs. Transplantation of the decellularized tissues into rat uteri revealed the successful regeneration of the uterine tissues with a 3-layer structure 30 days after the transplantation. Moreover, a lot of epithelial gland tissue and Ki67 positive cells were detected. Immunohistochemical analyses showed that the regenerated tissues have a normal response to ovarian hormone for pregnancy. The subsequent pregnancy test after 30 days transplantation revealed successful pregnancy for both the SDS and HHP groups. These findings indicate that the decellularized matrix from the uterine tissue can be a potential scaffold for uterine regeneration.

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“…To evaluate VEGF expression, the number of capillary vessels and proliferation cells was counted and averaged from at least 3 randomly selected fields under a magnification of 400× [12].…”

Section: Immunhistochemical Evaluationmentioning

confidence: 99%

Effect of stem cell application on Asherman Syndrome, an experimental rat model

Kılıç

Yüksel

Pınarlı³

et al. 2013

Fertility and Sterility

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Regeneration of uterine horns in rats by collagen scaffolds loaded with collagen-binding human basic fibroblast growth factor

Cited by 142 publications

References 30 publications

Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model

Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model

Application of Detergents or High Hydrostatic Pressure as Decellularization Processes in Uterine Tissues and Their Subsequent Effects on In Vivo Uterine Regeneration in Murine Models

Effect of stem cell application on Asherman Syndrome, an experimental rat model

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