Regeneration selection improves transformation efficiency in narrow-leaf lupin

Barker, Susan J.; Si, Ping; Hodgson, Leon Marc; Ferguson-Hunt, Margo; Khentry, Yuphin; Krishnamurthy, Priya; Averis, Susana; Mebus, Katharina; O’Lone, Claire; Dalugoda, Dumindi; Koshkuson, Nur; Faithfull, Troy; Jackson, J. F.; Erskine, William

doi:10.1007/s11240-016-0992-7

Cited by 14 publications

(9 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study provided novel evidences supporting the concept that RAP2.7 may control low-alkaloid iucundus phenotype, however, further evidence would require cis -trans tests. Nonetheless, such studies are hampered by very low transformation efficiency in narrow-leafed lupin [70].…”

Section: Resultsmentioning

confidence: 99%

Candidate Domestication-Related Genes Revealed by Expression Quantitative Trait Loci Mapping of Narrow-Leafed Lupin (Lupinus angustifolius L.)

Plewiński

Książkiewicz

Rychel-Bielska

et al. 2019

IJMS

View full text Add to dashboard Cite

The last century has witnessed rapid domestication of the narrow-leafed lupin (Lupinus angustifolius L.) as a grain legume crop, exploiting discovered alleles conferring low-alkaloid content (iucundus), vernalization independence (Ku and Julius), and reduced pod shattering (lentus and tardus). In this study, a L. angustifolius mapping population was subjected to massive analysis of cDNA ends (MACE). The MACE yielded 4185 single nucleotide polymorphism (SNP) markers for linkage map improvement and 30,595 transcriptomic profiles for expression quantitative trait loci (eQTL) mapping. The eQTL highlighted a high number of cis- and trans-regulated alkaloid biosynthesis genes with gene expression orchestrated by a regulatory agent localized at iucundus locus, supporting the concept that ETHYLENE RESPONSIVE TRANSCRIPTION FACTOR RAP2-7 may control low-alkaloid phenotype. The analysis of Ku shed light on the vernalization response via FLOWERING LOCUS T and FD regulon in L. angustifolius, providing transcriptomic evidence for the contribution of several genes acting in C-repeat binding factor (CBF) cold responsiveness and in UDP-glycosyltransferases pathways. Research on lentus selected a DUF1218 domain protein as a candidate gene controlling the orientation of the sclerified endocarp and a homolog of DETOXIFICATION14 for purplish hue of young pods. An ABCG transporter was identified as a hypothetical contributor to sclerenchyma fortification underlying tardus phenotype.

show abstract

Section: Resultsmentioning

confidence: 99%

Candidate Domestication-Related Genes Revealed by Expression Quantitative Trait Loci Mapping of Narrow-Leafed Lupin (Lupinus angustifolius L.)

Plewiński

Książkiewicz

Rychel-Bielska

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…In such a scenario, deletion of two copies of the BES1 binding site motif via the 1,208‐bp INDEL genotype would be sufficient to elevate LanFTc1 expression to an intermediate level, and deletion of all three motifs via the 1,423‐ or 5,162‐bp INDELs would fully derepress expression. With genome editing tools, such as the CRISPR/Cas‐9 system (Bortesi & Fischer, ), and more efficient transformation protocols in narrow‐leafed lupin (Barker et al, ), it may be feasible to modify BES1 binding site motifs in the wild‐type sequence to test the validity of this hypothesis in the future. If BES1 has a role in regulating LanFTc1 , BES1's known involvement within the photoperiodic pathway could explain why cultivars with the 1,423‐bp deletion are also less responsive to inductive long days than wild‐types without the large deletion in the promoter region of LanFTc1 (J. D. Berger, unpublished data).…”

Section: Discussionmentioning

confidence: 99%

“…As has been demonstrated in Arabidopsis, increasing from three to four tandem repeats of a 7-bp motif for CYCLING DOF FACTOR 1 in the promoter of CO increases the day-time repression of this gene and significantly delays flowering time (Rosas et al, 2014). We identified motifs for the binding site of a single transcription factor, named BES1, present once within the C H and twice within the C M critical regions (Tables 2, S4, and S5), yet nowhere else in the 5' UTR and 5' regulatory region of (Bortesi & Fischer, 2015), and more efficient transformation protocols in narrow-leafed lupin (Barker et al, 2016), it may be feasible to modify BES1 binding site motifs in the wild-type sequence to test the validity of this hypothesis in the future. If BES1 has a role in regulating LanFTc1, BES1's known involvement within the photoperiodic pathway could explain why cultivars with the 1,423-bp deletion are also less responsive to inductive long days than wild-types without the large deletion in the promoter region of LanFTc1 (J. D. Berger, unpublished data).…”

Section: Assessment Of Linkage Disequilibrium In the Lanftc1 Genomimentioning

confidence: 97%

INDEL variation in the regulatory region of the major flowering time gene LanFTc1 is associated with vernalization response and flowering time in narrow‐leafed lupin (Lupinus angustifolius L.)

Taylor

Kamphuis

Zhang

et al. 2018

Plant Cell & Environment

View full text Add to dashboard Cite

Narrow-leafed lupin (Lupinus angustifolius L.) cultivation was transformed by 2 dominant vernalization-insensitive, early flowering time loci known as Ku and Julius (Jul), which allowed expansion into shorter season environments. However, reliance on these loci has limited genetic and phenotypic diversity for environmental adaptation in cultivated lupin. We recently predicted that a 1,423-bp deletion in the cis-regulatory region of LanFTc1, a FLOWERING LOCUS T (FT) homologue, derepressed expression of LanFTc1 and was the underlying cause of the Ku phenotype. Here, we surveyed diverse germplasm for LanFTc1 cis-regulatory variation and identified 2 further deletions of 1,208 and 5,162 bp in the 5' regulatory region, which overlap the 1,423-bp deletion. Additionally, we confirmed that no other polymorphisms were perfectly associated with vernalization responsiveness. Phenotyping and gene expression analyses revealed that Jul accessions possessed the 5,162-bp deletion and that the Jul and Ku deletions were equally capable of removing vernalization requirement and up-regulating gene expression. The 1,208-bp deletion was associated with intermediate phenology, vernalization responsiveness, and gene expression and therefore may be useful for expanding agronomic adaptation of lupin. This insertion/deletion series may also help resolve how the vernalization response is mediated at the molecular level in legumes.

show abstract

“…The function of candidate genes may be studied by genetic transformation of lupin, the primary method being Agrobacterium tumefaciens -mediated transformation of wounded seedling shoot apical meristems to generate transgenic shoots ( Pigeaire et al, 1997 ). While transformation efficiencies are low due to low survival and chimeric nature of T 0 plants, this method has been successful in generating transgenic NLL ( Molvig et al, 1997 ; Pigeaire et al, 1997 ; Wijayanto et al, 2009 ; Tabe et al, 2010 ; Atkins et al, 2011 ; Barker et al, 2016 ), L. luteus ( Li et al, 2000 ; Pniewski et al, 2006 ), and L. mutabilis ( Babaoglu et al, 2000 ; Polowick et al, 2014 ) to confer various traits, with recent modifications improving this transformation method for NLL ( Nguyen et al, 2016a , b ). For L. albus , however, A. tumefaciens -mediated transformation has been unsuccessful and as such hairy root transformation using A. rhizogenes ( Uhde-Stone et al, 2005 ; Sbabou et al, 2010 ; Cheng et al, 2011 ) and VIGS using the Peanut stunt virus vector ( Yamagishi et al, 2015 ) have been used to study gene function in this species.…”

Section: Future Prospectsmentioning

confidence: 99%

Quinolizidine Alkaloid Biosynthesis in Lupins and Prospects for Grain Quality Improvement

et al. 2017

View full text Add to dashboard Cite

Quinolizidine alkaloids (QAs) are toxic secondary metabolites found within the genus Lupinus, some species of which are commercially important grain legume crops including Lupinus angustifolius (narrow-leafed lupin, NLL), L. luteus (yellow lupin), L. albus (white lupin), and L. mutabilis (pearl lupin), with NLL grain being the most largely produced of the four species in Australia and worldwide. While QAs offer the plants protection against insect pests, the accumulation of QAs in lupin grain complicates its use for food purposes as QA levels must remain below the industry threshold (0.02%), which is often exceeded. It is not well understood what factors cause grain QA levels to exceed this threshold. Much of the early work on QA biosynthesis began in the 1970–1980s, with many QA chemical structures well-characterized and lupin cell cultures and enzyme assays employed to identify some biosynthetic enzymes and pathway intermediates. More recently, two genes associated with these enzymes have been characterized, however, the QA biosynthetic pathway remains only partially elucidated. Here, we review the research accomplished thus far concerning QAs in lupin and consider some possibilities for further elucidation and manipulation of the QA pathway in lupin crops, drawing on examples from model alkaloid species. One breeding strategy for lupin is to produce plants with high QAs in vegetative tissues while low in the grain in order to confer insect resistance to plants while keeping grain QA levels within industry regulations. With the knowledge achieved on alkaloid biosynthesis in other plant species in recent years, and the recent development of genomic and transcriptomic resources for NLL, there is considerable scope to facilitate advances in our knowledge of QAs, leading to the production of improved lupin crops.

show abstract

Regeneration selection improves transformation efficiency in narrow-leaf lupin

Cited by 14 publications

References 13 publications

Candidate Domestication-Related Genes Revealed by Expression Quantitative Trait Loci Mapping of Narrow-Leafed Lupin (Lupinus angustifolius L.)

Candidate Domestication-Related Genes Revealed by Expression Quantitative Trait Loci Mapping of Narrow-Leafed Lupin (Lupinus angustifolius L.)

INDEL variation in the regulatory region of the major flowering time gene LanFTc1 is associated with vernalization response and flowering time in narrow‐leafed lupin (Lupinus angustifolius L.)

Quinolizidine Alkaloid Biosynthesis in Lupins and Prospects for Grain Quality Improvement

Contact Info

Product

Resources

About