Regenerative and Cell Therapy

Keating, Armand; Ka, Dicke; Gorin, Norbert‐Claude; Weber, R.; Graf, H.

doi:10.1007/b138167

Ernst Schering Research Foundation Workshop 2005

DOI: 10.1007/b138167

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Regenerative and Cell Therapy

Armand Keating¹,

Dicke Ka²,

Norbert‐Claude Gorin³

et al.

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Effects of cell detachment methods on the dielectric properties of adherent and suspension cells

2015

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Cell analyses such as flow cytometry, dielectrophoresis, and some patch-clamp techniques require that cells be in monodisperse suspension in order for analysis to occur. Where cells have a normally adherent phenotype in vivo, this requires that cells be removed from the surface of the culture flask or vessel. This can be achieved in many ways, but most commonly either by the use of a dissociation medium of some form, or by scraping the cells from the surface. Both methods have potential drawbacks; chemical methods may alter the properties of the cells to such a degree that the measurement might be regarded as meaningless, whilst scraping could cause physical damage to the structure of the cells. In this paper, we use dielectrophoresis to analyse the electrical properties of two adherent cell lines detached by multiple methods, and compare these against a control cell line of suspension cells, examined in both the native state and when subject to the same chemical treatments. The results indicate that most chemical agents do not alter the electrophysiology of cells directly, though they may trigger some potential cell deterioration processes such as apoptosis in the cells. This can be observed in the production of apoptotic body-like particles and the alteration of cytoplasmic conductivity (which has been associated with apoptotic water efflux). However, cells detached by scraping exhibited statistically significant differences in their electrophysiological properties when compared to those detached by the chemical methods, indicating that this method is unsuitable for detachment of adherent cells prior to analysis of isolates suspension cells.

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Effects of cell detachment methods on the dielectric properties of adherent and suspension cells

2015

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Polymer-Based Scaffolds for Soft-Tissue Engineering

et al. 2020

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Biomaterials have been used since ancient times. However, it was not until the late 1960s when their development prospered, increasing the research on them. In recent years, the study of biomaterials has focused mainly on tissue regeneration, requiring a biomaterial that can support cells during their growth and fulfill the function of the replaced tissue until its regeneration. These materials, called scaffolds, have been developed with a wide variety of materials and processes, with the polymer ones being the most advanced. For this reason, the need arises for a review that compiles the techniques most used in the development of polymer-based scaffolds. This review has focused on three of the most used techniques: freeze-drying, electrospinning and 3D printing, focusing on current and future trends. In addition, the advantages and disadvantages of each of them have been compared.

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Regenerative and Cell Therapy

Cited by 2 publications

References 0 publications

Effects of cell detachment methods on the dielectric properties of adherent and suspension cells

Effects of cell detachment methods on the dielectric properties of adherent and suspension cells

Polymer-Based Scaffolds for Soft-Tissue Engineering

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