Regenerative capacity of the dystrophic (mdx) diaphragm after induced injury

Matecki, Stéfan; Guibinga, Ghiabe H.; Petrof, Basil J.

doi:10.1152/ajpregu.00146.2004

Cited by 32 publications

(33 citation statements)

References 55 publications

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“…Taking into account the similarity of cell differentiation pathways during normal development and regeneration, we used immunolabeling for the following protein markers of myogenesis: (1) MyoD and myogenin, the myogenic regulatory factors expressed by committed myoblasts before and following fusion (Yablonka-Reuveni and Paterson 2001; Paoni et al 2002;Pownall et al 2002;Emerson and Hauschka 2004), (2) the embryonic isoform of myosin expressed in nascent muscle fibers (Paoni et al 2002;Matecki et al 2004), and (3) the isoform of neural cell adhesion molecule (NCAM) typical of activated myogenic cells and early myotubes (Mesires and Doumit 2002;Crameri et al 2004).…”

Section: Resultsmentioning

confidence: 99%

Differentiation of activated satellite cells in denervated muscle following single fusions in situ and in cell culture

Borisov

Dedkov

Carlson

2005

Histochem Cell Biol

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Satellite cells represent a cellular source of regeneration in adult skeletal muscle. It remains unclear why a large pool of stem myoblasts in denervated muscle does not compensate for the loss of muscle mass during post-denervation atrophy. In this study, we present evidence that satellite cells in long-term denervated rat muscle are able to activate synthesis of contractile proteins after single fusions in situ. This process of early differentiation leads to formation of abnormally diminutive myotubes. The localization of such dwarf myotubes beneath the intact basal lamina on the surface of differentiated muscle fibers shows that they form by fusion of neighboring satellites or by the progeny of a single satellite cell following one or two mitotic divisions. We demonstrated single fusions of myoblasts using electron microscopy, immunocytochemical labeling and high resolution confocal digital imaging. Sequestration of nascent myotubes by the rapidly forming basal laminae creates a barrier that limits further fusions. The recruitment of satellite cells in the formation of new muscle fibers results in a progressive decrease in their local densities, spatial separation and ultimate exhaustion of the myogenic cell pool. To determine whether the accumulation of aberrant dwarf myotubes is explained by the intrinsic decline of myogenic properties of satellite cells, or depends on their spatial separation and the environment in the tissue, we studied the fusion of myoblasts isolated from normal and denervated muscle in cell culture. The experiments with a culture system demonstrated that the capacity of myoblasts to synthesize contractile proteins without serial fusions depended on cell density and the availability of partners for fusion. Satellite cells isolated from denervated muscle and plated at fusion-permissive densities progressed through the myogenic program and actively formed myotubes, which shows that their myogenic potential is not considerably impaired. The results of this study suggest that under conditions of denervation, progressive spatial separation and confinement of many satellite cells within the endomysial tubes of atrophic muscle fibers and progressive interstitial fibrosis are the important factors that prevent their normal differentiation. Our findings also provide an explanation of why denervated muscle partially and temporarily is able to restore its functional capacity following injury and regeneration: the release of satellite cells from their sublaminal location provides the necessary space for a more active regenerative process.

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Section: Resultsmentioning

confidence: 99%

Differentiation of activated satellite cells in denervated muscle following single fusions in situ and in cell culture

Borisov

Dedkov

Carlson

2005

Histochem Cell Biol

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“…Calpains 1 and 2 are ubiquitous non-lysosomal proteases, and are unregulated early in the time course of VIDD [6][7][8]. The calpains are involved in regulation of the cell cycle, apoptosis, and cytoskeletal reorganization, and also play roles in muscle wasting by initiating myofibrillar disassembly, which causes the myofibrillar proteins to become susceptible to further degradation by the proteasome [21,22].…”

Section: Discussionmentioning

confidence: 99%

“…The sarcolemma of normal myofibers is known to be impermeable to Procion orange, and damaged sarcolemmal fibers can be identified by their inability to exclude Procion orange from the cytoplasm [6].…”

Section: Measurement Of Sarcolemmal Injury In the Diaphragmmentioning

confidence: 99%

Effects of Lower Tidal Volume on Ventilator-Induced Diaphragmatic Dysfunction

DH¹

2017

JLPRR

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Purpose: Mechanical ventilation (MV) is one of the most important treatment to achieve sufficient alveolar ventilation in patients with lack of adequate pulmonary gas exchange. This study was performed to evaluate the effects of tidal volume ventilation to diaphragmatic dysfunction by both histological evaluation and the main protease pathway after MV with a murine ventilator induced diaphragmatic dysfunction (VIDD) model. Materials and Methods:Healthy male C57/BL6 mice (10 - 12 weeks old, 25 - 30 g) were randomly divided into 3 experimental groups: high tidal volume MV for 6 hours (HTV group, n = 6), low tidal volume MV for 6 hours (LTV group, n = 6), and control (Control group, n = 6). Arterial blood gas analysis, examination of diaphragmatic contractile properties, histological evaluation, and biochemical evaluation of main proteolysis pathways were performed in all three groups. Results:The results of arterial blood gas analysis were comparable among the three groups and all of them were within the normal ranges. Diaphragmatic force production was lower in the HTV group compared to the LTV and control group, respectively. Diaphragmatic force production in the LTV group was higher than that in the HTV group but only slightly reduced compared to the control group. There were no histological differences were found between the groups. No alterations in the level of calpain 1 or 2 were observed among the group, respectively. Conclusion:Lower tidal volume ventilation partially reduces ventilator-induced diaphragmatic dysfunction. Further studies are needed to determine the mechanism.

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“…The laparoscopic procedure has been successfully used in interventions in the diaphragm 12,13 . However, until the present moment there are no studies using laparoscopic procedure for local injection in the mdx diaphragm.…”

Section: Discussionmentioning

confidence: 99%

“…Matecki et al 12 , used the laparoscopy to expose the abdominal surface of the right hemidiaphragm in the mdx and wild-type (C57BL10) and induce a massive necrosis with a topical application of notexin. After surgery, the authors reported that there were no complications, confirming the mdx tolerance for this type of intervention.…”

Section: Discussionmentioning

confidence: 99%

Laparoscopic guided local injection in the X-linked muscular dystrophy mouse (mdx) diaphragm. An advance in experimental therapies for Duchenne Muscular Dystrophy

Lessa

Carvalho²,

Spagnolo³

et al. 2014

Acta Cir. Bras.

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PURPOSE:To investigate the development of a laparoscopy technique for local injection into the X-linked muscular dystrophy (mdx) diaphragm. METHODS:It was used 10 mice Balb/C57 and 5 mdx mice and three differents decubitus type were tested: the right lateral, supine, and supine decubitus with 20 degrees elevation of the forelimb. Abdominal caudal face and the 10 intercostal space were tested as spot to introduce the needle into the diaphragm. RESULTS:Supine position with elevation of 20 degrees forelimb and the 10th intercostal space are the beneficial position to apply a local injection. CONCLUSION:It was proved to be possible to perform the laparoscopy technique in the X-linked muscular dystrophy diaphragm and this requires a specific position and technique during the surgery.

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Regenerative capacity of the dystrophic (mdx) diaphragm after induced injury

Abstract: Regenerative capacity of the dystrophic (mdx) diaphragm after induced injury.

Cited by 32 publications

References 55 publications

Differentiation of activated satellite cells in denervated muscle following single fusions in situ and in cell culture

Differentiation of activated satellite cells in denervated muscle following single fusions in situ and in cell culture

Effects of Lower Tidal Volume on Ventilator-Induced Diaphragmatic Dysfunction

Laparoscopic guided local injection in the X-linked muscular dystrophy mouse (mdx) diaphragm. An advance in experimental therapies for Duchenne Muscular Dystrophy

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