Regenerative effect of expired platelet concentrates in human therapy: An update

Burnouf, Thierry; Goubran, Hadi

doi:10.1016/j.transci.2022.103363

Cited by 4 publications

(1 citation statement)

References 42 publications

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“…The platelet concentration in PC is 4-6 times (600-2400 × 10 9 ) higher than that in whole blood [6]. Platelet alpha granules contain several growth factors, such as epidermal growth factor (EGF), platelet-derived growth factor (PDGF), transforming growth factor (TGF)-β1, insulin-like growth factor (IGF)-I, IGF-II, vascular endothelial growth factor (VEGF), endothelial cell growth factor (ECGF), and basic fibroblast growth factor (bFGF) [7]. These growth factors, both in terms of their concentrations and types, can induce clinical regenerative effects.…”

Section: Introductionmentioning

confidence: 99%

Expired Platelet Concentrate Up-Cycling: Growth Factor-Rich Bioproduct Preparation for FBS Substitute

Lee,

Chun,

Yoon

et al. 2023

JCM

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Due to the short storage period, large quantities of platelet concentrate (PC) are expiring. The expired PC cannot be injected into a blood vessel, but the activity of bioactive molecules, especially growth factors, is still preserved. In this paper, we organized a process to obtain a growth factor-rich bioproduct for use as a supplement in human cell culture by optimizing freezing, thawing, and sterilization conditions. Each unit of PC displayed visual differences, diverse biochemical values, and growth factor concentrations. To minimize lot-to-lot variation, we pooled a minimum of 10 PC units. The concentrations of growth factors were maximized through five freeze–thaw cycles for 12 h at −80 °C for freezing and for 5 min at 36 °C for thawing. We used a cell strainer with 40 µm pores, followed by a 0.45 μm filter and a 0.22 μm filter sequentially to sterilize the bioproduct with minimizing loss. The obtained growth factors remained stable for 4–6 h at room temperature (23 °C), 24 h at 4 °C, and 12 months at −80 °C. Cellular responses to the growth factor-rich bioproduct were tested with primary human renal proximal tubule epithelial cells. The cells exhibited a significantly increased growth rate, compared to the fetal bovine serum (FBS)-treated control group. The cells maintained their characteristic cuboidal shape, and stem cells and renal progenitor cells also preserved their genetic characteristics during culture. Therefore, the growth factor-rich bioproduct isolated from expired PC through our process can be used as a medium supplement to replace FBS in human cell culture for clinical application.

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Section: Introductionmentioning

confidence: 99%