Regenerative Medicine and Tissue Engineering in Reproductive Medicine

Cervelló, Irene; Medrano, José V.; Simón, Carlos

doi:10.1016/b978-0-12-800548-4.00010-3

Cited by 2 publications

(2 citation statements)

References 127 publications

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“…Bioengineering methods used in modeling mammalian tissues include decellularizing tissues and organs to generate high-quality ECM scaffolds that support in vitro cell culture [21][22][23][24] and contain structural proteins, growth factors, and other signaling molecules involved in cellular processes such as homeostasis and tissue repair [25]. Concerning the gynecological field, different forms of decellularized ECM are used to enhance uterine repair [26,27] or optimize in vitro embryo culture [28,29], supporting their use in reproductive medicine research.…”

Section: Introductionmentioning

confidence: 99%

Improved Models of Human Endometrial Organoids Based on Hydrogels from Decellularized Endometrium

Francés-Herrero

Juárez‐Barber

Campo

et al. 2021

JPM

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Organoids are three-dimensional (3D) multicellular tissue models that mimic their corresponding in vivo tissue. Successful efforts have derived organoids from primary tissues such as intestine, liver, and pancreas. For human uterine endometrium, the recent generation of 3D structures from primary endometrial cells is inspiring new studies of this important tissue using precise preclinical models. To improve on these 3D models, we decellularized pig endometrium containing tissue-specific extracellular matrix and generated a hydrogel (EndoECM). Next, we derived three lines of human endometrial organoids and cultured them in optimal and suboptimal culture expansion media with or without EndoECM (0.01 mg/mL) as a soluble additive. We characterized the resultant organoids to verify their epithelial origin, long-term chromosomal stability, and stemness properties. Lastly, we determined their proliferation potential under different culture conditions using proliferation rates and immunohistochemical methods. Our results demonstrate the importance of a bioactive environment for the maintenance and proliferation of human endometrial organoids.

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Section: Introductionmentioning

confidence: 99%

Improved Models of Human Endometrial Organoids Based on Hydrogels from Decellularized Endometrium

Francés-Herrero

Juárez‐Barber

Campo

et al. 2021

JPM

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“…Retinal disorders directly affect vision; therefore, retinal tissue engineering is fundamental. [3,[9][10][11] To understand the effective mechanisms in this process, it is better to compare growth factors' interaction with each other and then select the most appropriate one.…”

Section: Introductionmentioning

confidence: 99%

An In-Silico Study on the Most Effective Growth Factors in Retinal Regeneration Utilizing Tissue Engineering Concepts

Beheshtizadeh

Baradaran‐Rafii

Sistani

et al. 2021

JOVR

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Purpose: Considering the significance of retinal disorders and the growing need to employ tissue engineering in this field, in-silico studies can be used to establish a cost-effective method. This in-silico study was performed to find the most effective growth factors contributing to retinal tissue engineering. Methods: In this study, a regeneration gene database was used. All 21 protein-coding genes participating in retinal regeneration were considered as a protein–protein interaction (PPI) network via the “STRING App” in “Cytoscape 3.7.2” software. The resultant graph possessed 21 nodes as well as 37 edges. Gene ontology (GO) analysis, as well as the centrality analysis, revealed the most effective proteins in retinal regeneration. Results: According to the biological processes and the role of each protein in different pathways, selecting the correct one is possible through the information that the network provides. Eye development, detection of the visible light, visual perception, photoreceptor cell differentiation, camera-type eye development, eye morphogenesis, and angiogenesis are the major biological processes in retinal regeneration. Based on the GO analysis, SHH, STAT3, FGFR1, OPN4, ITGAV, RAX, and RPE65 are effective in retinal regeneration via the biological processes. In addition, based on the centrality analysis, four proteins have the greatest influence on retinal regeneration: SHH, IGF1, STAT3, and ASCL1. Conclusion: With the intention of applying the most impressive growth factors in retinal engineering, it seems logical to pay attention to SHH, STAT3, and RPE65. Utilizing these proteins can lead to fabricate high efficiency engineered retina via all aforementioned biological processes.

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Regenerative Medicine and Tissue Engineering in Reproductive Medicine

Cited by 2 publications

References 127 publications

Improved Models of Human Endometrial Organoids Based on Hydrogels from Decellularized Endometrium

Improved Models of Human Endometrial Organoids Based on Hydrogels from Decellularized Endometrium

An In-Silico Study on the Most Effective Growth Factors in Retinal Regeneration Utilizing Tissue Engineering Concepts

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