Regenerative tissue filler for breast conserving surgery and other soft tissue restoration and reconstruction needs

Puls, Theodore J; Fisher, Carla S.; Cox, Abigail; Plantenga, Jeannie M.; McBride, Emma L.; Anderson, Jennifer L.; Goergen, Craig J.; Bible, Melissa; Moller, Tracy; Voytik‐Harbin, Sherry L.

doi:10.1038/s41598-021-81771-x

Cited by 21 publications

(28 citation statements)

References 68 publications

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“…Collagen IV fragments 21 AGEKSYGKPC 30 (1), 41 CFPEKGARGR 50 (2), 81 PGLLGPYG PK90 (3), 161 LAPGSFKGMK 170 (4), 221 GFQGEKGVKG 230 (5), 251 GFPKGKKGSK 260 (6), 311 GPPGQQGKKG 320 (7), 451 NKESGFPGLR 460 (8), 471 LKGIKGDSGF 480 (9), 511 KGARGDR GSG 520 (10), 541 KGKKGEPILS 550 (11), 641 PGQQGLPGSK 650 (12), 671 PGFPGPKGSR 680 (13), 711 GFPGPRGEKG 720 (14), 721…”

Section: General Informationmentioning

confidence: 99%

“…From the pool of biopolymers, collagen is the most used because it is the main building block of the extracellular matrix (ECM). Collagen materials have been used in the regeneration of osteochondral defects [3], connective tissue [4], adipose tissue [5,6], mammary glands [7] and others [8][9][10][11][12]. In addition, in the case of scaffolds based on natural materials, including collagens [13], the pore architecture can be designed to mimic the anisotropic nature of ECM in native tissues [14,15].…”

Section: Introductionmentioning

confidence: 99%

“…DMT/NMM/TosO − (1.239 g, 3.0 mmol) and NMM (0.66 mL, 6.0 mmol). HPLC ( • Synthesis of 311 GPPGQQGKKG 320 (7) Starting materials: resin (1.0 g, 1.0 mmol) and for each coupling: Fmoc-Gly-OH (0.892 g, 3.0 mmol), Fmoc-Lys(Boc)-OH (1.405 g, 3.0 mmol), Fmoc-Lys(Boc)-OH (1.405 g, 3.0 mmol), Fmoc-Gly-OH (0.892 g, 3.0 mmol), Fmoc-Gln(Trt)-OH (1.832 g, 3.0 mmol), Fmoc-Gln(Trt)-OH (1.832 g, 3.0 mmol), Fmoc-Gly-OH (0.892 g, 3.0 mmol), Fmoc-Pro-OH (1.012 g, 3.0 mmol), Fmoc-Pro-OH (1.012 g, 3.0 mmol), Fmoc-Gly-OH (0.892 g, 3.0 mmol). DMT/NMM/TosO − (1.239 g, 3.0 mmol) and NMM (0.66 mL, 6.0 mmol).…”

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confidence: 99%

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Screening of Self-Assembling of Collagen IV Fragments into Stable Structures Potentially Useful in Regenerative Medicine

Kolasa

Galita

Majsterek

et al. 2021

IJMS

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The aim of the research was to check whether it is possible to use fragments of type IV collagen to obtain, as a result of self-assembling, stable spatial structures that could be used to prepare new materials useful in regenerative medicine. Collagen IV fragments were obtained by using DMT/NMM/TosO− as a coupling reagent. The ability to self-organize and form stable spatial structures was tested by the CD method and microscopic techniques. Biological studies covered: resazurin assay (cytotoxicity assessment) on BJ, BJ-5TA and C2C12 cell lines; an alkaline version of the comet assay (genotoxicity), Biolegend Legendplex human inflammation panel 1 assay (SC cell lines, assessment of the inflammation activity) and MTT test to determine the cytotoxicity of the porous materials based on collagen IV fragments. It was found that out of the pool of 37 fragments (peptides 1–33 and 2.1–2.4) reconstructing the outer sphere of collagen IV, nine fragments (peptides: 2, 4, 5, 6, 14, 15, 25, 26 and 30), as a result of self-assembling, form structures mimicking the structure of the triple helix of native collagens. The stability of spatial structures formed as a result of self-organization at temperatures of 4 °C, 20 °C, and 40 °C was found. The application of the MST method allowed us to determine the Kd of binding of selected fragments of collagen IV to ITGα1β1. The stability of the spatial structures of selected peptides made it possible to obtain porous materials based on their equimolar mixture. The formation of the porous materials was found for cross-linked structures and the material stabilized only by weak interactions. All tested peptides are non-cytotoxic against all tested cell lines. Selected peptides also showed no genotoxicity and no induction of immune system responses. Research on the use of porous materials based on fragments of type IV collagen, able to form stable spatial structures as scaffolds useful in regenerative medicine, will be continued.

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Section: General Informationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Screening of Self-Assembling of Collagen IV Fragments into Stable Structures Potentially Useful in Regenerative Medicine

Kolasa

Galita

Majsterek

et al. 2021

IJMS

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“…Ultrasound is also available for studying the in vivo degradation of other types of biomaterials. In a recent study, ultrasonography was performed to assess the degradation of scaffold-forming collagen implanted in Pig breasts [ 25 ]. It can be inferred that ultrasound can be applied when the echo of the material is different from the tissue of the implantation site or there is a gap between the implantation sites.…”

Section: Discussionmentioning

confidence: 99%

Application of an ultrasound semi-quantitative assessment in the degradation of silk fibroin scaffolds in vivo

et al. 2021

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Background Research on the degradation of silk fibroin (SF) scaffolds in vivo lacks uniform and effective standards and experimental evaluation methods. This study aims to evaluate the application of ultrasound in assessing the degradation of SF scaffolds. Methods Two groups of three-dimensional regenerated SF scaffolds (3D RSFs) were implanted subcutaneously into the backs of Sprague-Dawley rats. B-mode ultrasound and hematoxylin and eosin (HE) staining were performed on days 3, 7, 14, 28, 56, 84, 112, 140, and 196. The cross-sectional areas for two groups of 3D RSFs that were obtained using these methods were semi-quantitatively analyzed and compared to evaluate the biodegradation of the implanted RSFs. Results The 3D RSFs in the SF-A group were wholly degraded at the 28th week after implantation. In contrast, the 3D RSFs in the SF-B group were completely degraded at the 16th week. Ultrasonic examination showed that the echoes of 3D RSFs in both groups gradually decreased with the increase of the implantation time. In the early stages of degradation, the echoes of the samples were higher than the echo of the muscle. In the middle of degeneration, the echoes were equal to the echo of the muscle. In the later stage, the echoes of the samples were lower than that of the muscle. The above changes in the SF-B group were earlier than those in the SF-A group. Semi-quantitative analysis of the cross-sectional areas detected using B-mode ultrasound revealed that the degradations of the two 3D RSF groups were significantly different. The degradation rate of the SF-B group was found to be higher than that of the SF-A group. This was consistent with the semi-quantitative detection results for HE staining. Regression analysis showed that the results of the B-mode ultrasound and HE staining were correlated in both groups, indicating that B-mode ultrasound is a reliable method to evaluate the SF scaffold degradation in vivo. Conclusions This study suggests that B-mode ultrasound can clearly display the implanted SF scaffolds non-invasively and monitor the degradation of the different SF scaffolds after implantation in living organisms in real-time.

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“…Aging features such as wrinkles and loss of skin elasticity can be rejuvenated through antiaging approaches, including dermal filling [68]. Soft tissue fillers based on ECM constituents have been reported to affect tissue regeneration [69,70]. ECM-based materials can be processed from native tissues to products through isolation, decellularization, and purification (Figure 1) [71].…”

Section: Types Of Ecm-based Materials For Biomedical Applicationsmentioning

confidence: 99%

Employing Extracellular Matrix-Based Tissue Engineering Strategies for Age-Dependent Tissue Degenerations

Hwang

Jang

2021

IJMS

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Tissues and organs are not composed of solely cellular components; instead, they converge with an extracellular matrix (ECM). The composition and function of the ECM differ depending on tissue types. The ECM provides a microenvironment that is essential for cellular functionality and regulation. However, during aging, the ECM undergoes significant changes along with the cellular components. The ECM constituents are over- or down-expressed, degraded, and deformed in senescence cells. ECM aging contributes to tissue dysfunction and failure of stem cell maintenance. Aging is the primary risk factor for prevalent diseases, and ECM aging is directly or indirectly correlated to it. Hence, rejuvenation strategies are necessitated to treat various age-associated symptoms. Recent rejuvenation strategies focus on the ECM as the basic biomaterial for regenerative therapies, such as tissue engineering. Modified and decellularized ECMs can be used to substitute aged ECMs and cell niches for culturing engineered tissues. Various tissue engineering approaches, including three-dimensional bioprinting, enable cell delivery and the fabrication of transplantable engineered tissues by employing ECM-based biomaterials.

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Regenerative tissue filler for breast conserving surgery and other soft tissue restoration and reconstruction needs

Cited by 21 publications

References 68 publications

Screening of Self-Assembling of Collagen IV Fragments into Stable Structures Potentially Useful in Regenerative Medicine

Screening of Self-Assembling of Collagen IV Fragments into Stable Structures Potentially Useful in Regenerative Medicine

Application of an ultrasound semi-quantitative assessment in the degradation of silk fibroin scaffolds in vivo

Employing Extracellular Matrix-Based Tissue Engineering Strategies for Age-Dependent Tissue Degenerations

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