Region‐specific meiotic recombination in <i>Schizosaccharomyces pombe</i>: the <i>rec11</i> gene

Li, Ywan Feng; Numata, Masayuki; Wahls, Wayne P.; Smith, Gerald R.

doi:10.1046/j.1365-2958.1997.2691632.x

Cited by 34 publications

(24 citation statements)

References 40 publications

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“…Part of the meiotic enhancement is caused by induction of meiotic recombination genes (27) and part may be because of Mts1͞Mts2 protein binding to M26 sites throughout the genome (15). Intergenic recombination in mts mutants is reduced as much as 50% in four intervals tested so far (N.K.…”

Section: Discussionmentioning

confidence: 99%

“…The amino acid sequences of tryptic fragments of Mts1 (amino acids 86-112, 130-158, 213-247) and Mts2 (amino acids 37-50, 52-65) were determined by Bill Lane at the Harvard Microchemistry Facility and were used to design degenerate oligonucleotide primers for PCR amplification (24). Products of PCR amplification of genomic DNA were cloned into pBluescriptKSIIϩ, sequenced (25) to confirm that they harbored the correct fragments, amplified, excised, and used as probes for colony hybridization (26) to identify multiple independent clones from a partial-digest, size-fractionated genomic DNA library (27). PCR analysis was used to identify one clone (pMts1-7) harboring a 4,376-bp insert with a centrally located mts1 gene and one clone (pMts2-3) harboring a 3,642-bp insert with a centrally located mts2 gene.…”

Section: Methodsmentioning

confidence: 99%

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Transcription factor Mts1/Mts2 (Atf1/Pcr1, Gad7/Pcr1) activates the M 26 meiotic recombination hotspot in Schizosaccharomyces pombe

Kon

Krawchuk

Warren

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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Homologous recombination hotspots increase the frequency of recombination in nearby DNA. The M26 hotspot in the ade6 gene of Schizosaccharomyces pombe is a meiotic hotspot with a discrete, cis-acting nucleotide sequence (5-ATGACGT-3) defined by extensive mutagenesis. A heterodimeric M26 DNA binding protein, composed of subunits Mts1 and Mts2, has been identified and purified 40,000-fold. Cloning, disruption, and genetic analyses of the mts genes demonstrate that the Mts1͞Mts2 heterodimer is essential for hotspot activity. This provides direct evidence that a specific trans-acting factor, binding to a cis-acting site with a unique nucleotide sequence, is required to activate this meiotic hotspot. Intriguingly, the Mts1͞Mts2 protein subunits are identical to the recently described transcription factors Atf1 (Gad7) and Pcr1, which are required for a variety of stress responses. However, we report differential dependence on the Mts proteins for hotspot activation and stress response, suggesting that these proteins are multifunctional and have distinct activities. Furthermore, ade6 mRNA levels are equivalent in hotspot and nonhotspot meioses and do not change in mts mutants, indicating that hotspot activation is not a consequence of elevated transcription levels. These findings suggest an intimate but separable link between the regulation of transcription and meiotic recombination. Other studies have recently shown that the Mts1͞Mts2 protein and M26 sites are involved in meiotic recombination elsewhere in the S. pombe genome, suggesting that these factors help regulate the timing and distribution of homologous recombination.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Transcription factor Mts1/Mts2 (Atf1/Pcr1, Gad7/Pcr1) activates the M 26 meiotic recombination hotspot in Schizosaccharomyces pombe

Kon

Krawchuk

Warren

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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153

256

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“…Region-specific trans-acting modifiers have been characterized in Schizosaccharomyces pombe (De Veaux et al 1992;De Veaux and Smith 1994;Li et al 1997;Krawchuk et al 1999;Pryce et al 2005) and Saccharomyces cerevisiae (Rockmill and Roeder 1990 Schuermann et al 2005). In S. pombe, rec10, a protein involved in the formation of lateral elements, is required for the activation of some but not all M26-containing recombination hot spots (Pryce et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Effects of trans-acting Genetic Modifiers on Meiotic Recombination Across the a1–sh2 Interval of Maize

2006

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Meiotic recombination rates are potentially affected by cis-and trans-acting factors, i.e., genotypespecific modifiers that do or do not reside in the recombining interval, respectively. Effects of trans modifiers on recombination across the $140-kb maize a1-sh2 interval of chromosome 3L were studied in the absence of polymorphic cis factors in three genetically diverse backgrounds into which a sequenceidentical a1-sh2 interval had been introgressed. Genetic distances across a1-sh2 varied twofold among genetic backgrounds. Although the existence of regions exhibiting high and low rates of recombination (hot and cold spots, respectively) was conserved across backgrounds, the absolute rates of recombination in these sequence-identical regions differed significantly among backgrounds. In addition, an intergenic hot spot had a higher rate of recombination as compared to the genome average rate of recombination in one background and not in another. Recombination rates across two genetic intervals on chromosome 1 did not exhibit the same relationships among backgrounds as was observed in a1-sh2. This suggests that at least some detected trans-acting factors do not equally affect recombination across the genome. This study establishes that trans modifier(s) polymorphic among genetic backgrounds can increase and decrease recombination in both genic and intergenic regions over relatively small genetic and physical intervals.

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“…The PCR primers had EcoRI restriction pH 6.4, containing 0.1 m 2-morpholinoethanesulfonic acid, 1 sites at their 5Ј-ends enabling the PCR products to be cloned mm EDTA, and 0.5 mm MgCl 2 . The spheroplasts were pelleted directly into the S. pombe vector, pFY20 (Li et al 1997). The and resuspended in an appropriate volume (normally 150-200 PCR primers were rec10 JS-F EcoRI (5Ј-GCGCGAATTCCTGCG l) of the sorbitol solution.…”

Section: )mentioning

confidence: 99%

Differential Activation of M26-Containing Meiotic Recombination Hot Spots in Schizosaccharomyces pombe

et al. 2005

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Certain genomic loci, termed hot spots, are predisposed to undergo genetic recombination during meiosis at higher levels relative to the rest of the genome. The factors that specify hot-spot potential are not well understood. The M26 hot spot of Schizosaccharomyces pombe is dependent on certain trans activators and a specific nucleotide sequence, which can function as a hot spot in a position-and orientation-independent fashion within ade6. In this report we demonstrate that a linear element (LE) component, Rec10, has a function that is required for activation of some, but not all, M26-containing hot spots and from this we propose that, with respect to hot-spot activity, there are three classes of M26-containing sequences. We demonstrate that the localized sequence context in which the M26 heptamer is embedded is a major factor governing whether or not this Rec10 function is required for full hot-spot activation. Furthermore, we show that the rec10-144 mutant, which is defective in full activation of ade6-M26, but proficient for activation of other M26-containing hot spots, is also defective in the formation of LEs, suggesting an intimate link between higher-order chromatin structure and local influences on hot-spot activation.

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Region‐specific meiotic recombination in Schizosaccharomyces pombe: the rec11 gene

Cited by 34 publications

References 40 publications

Transcription factor Mts1/Mts2 (Atf1/Pcr1, Gad7/Pcr1) activates the M 26 meiotic recombination hotspot in Schizosaccharomyces pombe

Transcription factor Mts1/Mts2 (Atf1/Pcr1, Gad7/Pcr1) activates the M 26 meiotic recombination hotspot in Schizosaccharomyces pombe

Effects of trans-acting Genetic Modifiers on Meiotic Recombination Across the a1–sh2 Interval of Maize

Differential Activation of M26-Containing Meiotic Recombination Hot Spots in Schizosaccharomyces pombe

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