Regional anticoagulation magnetic artificial blood vessels constructed by heparin-PLCL core–shell nanofibers for rapid deployment of veno-venous bypass

Liu, Peng; Yang, Lifei; Shi, Aihua; Qian, Ye-Rong; Liu, Xin; Dong, Dinghui; Zhang, Xufeng; Lv, Yi; Xiang, Jun

doi:10.1039/d2bm00205a

Cited by 4 publications

(7 citation statements)

References 41 publications

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“…AVGs were prepared as previously described ( Liu et al, 2022 ) with modifications. As shown in Figure 2 , a 3D printer (Xiao Fang ONE, Exquisitely 3D, China) was used to fabricate electrospun collectors.…”

Section: Methodsmentioning

confidence: 99%

“…To investigate platelet adhesion, fresh anticoagulated rabbit blood was centrifuged to obtain platelet-rich plasma (PRP) ( Liu et al, 2022 ). A 1.0 × 1.0 cm 2 sample was immersed in PRP and incubated at 37°C for 1 h. The morphology and number of platelets adhered to the electrospun membrane were observed and quantified using SEM.…”

Section: Methodsmentioning

confidence: 99%

“…To address the challenges of small-diameter AVGs, innovations like coaxial electrospinning, decellularization, freeze-drying and 3D printing have been developed ( Darie-Niță et al, 2022 ). Coaxial electrospinning in particular shows promise for nanofibrous vascular grafts ( Laktionov et al, 2014 ; Yin et al, 2017 ; Liu et al, 2022 ). This method enables the incorporation of anticoagulant agents ( Huang et al, 2013 ; Kuang et al, 2018 ), within the core-shell structure of the nanofibers, thus enhancing their antithrombotic properties.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced hemocompatibility and rapid magnetic anastomosis of electrospun small-diameter artificial vascular grafts

Liu,

Yang

et al. 2024

Front. Bioeng. Biotechnol.

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Background: Small-diameter (<6 mm) artificial vascular grafts (AVGs) are urgently required in vessel reconstructive surgery but constrained by suboptimal hemocompatibility and the complexity of anastomotic procedures. This study introduces coaxial electrospinning and magnetic anastomosis techniques to improve graft performance.Methods: Bilayer poly(lactide-co-caprolactone) (PLCL) grafts were fabricated by coaxial electrospinning to encapsulate heparin in the inner layer for anticoagulation. Magnetic rings were embedded at both ends of the nanofiber conduit to construct a magnetic anastomosis small-diameter AVG. Material properties were characterized by micromorphology, fourier transform infrared (FTIR) spectra, mechanical tests, in vitro heparin release and hemocompatibility. In vivo performance was evaluated in a rabbit model of inferior vena cava replacement.Results: Coaxial electrospinning produced PLCL/heparin grafts with sustained heparin release, lower platelet adhesion, prolonged clotting times, higher Young’s modulus and tensile strength versus PLCL grafts. Magnetic anastomosis was significantly faster than suturing (3.65 ± 0.83 vs. 20.32 ± 3.45 min, p < 0.001) and with higher success rate (100% vs. 80%). Furthermore, magnetic AVG had higher short-term patency (2 days: 100% vs. 60%; 7 days: 40% vs. 0%) but similar long-term occlusion as sutured grafts.Conclusion: Coaxial electrospinning improved hemocompatibility and magnetic anastomosis enhanced implantability of small-diameter AVG. Short-term patency was excellent, but further optimization of anticoagulation is needed for long-term patency. This combinatorial approach holds promise for vascular graft engineering.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhanced hemocompatibility and rapid magnetic anastomosis of electrospun small-diameter artificial vascular grafts

Liu,

Yang

et al. 2024

Front. Bioeng. Biotechnol.

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“…The structure, diameter, and mechanical properties of the nanofibers produced by electrospining polymer solutions can be easily tuned by adjusting various operating parameters. In addition, electrospinning can also use different polymers or their combined solutions to build grafts with different properties and it is possible to construct core-shell structures [26].…”

Section: Introductionmentioning

confidence: 99%

Construction of PCL-collagen@PCL@PCL-gelatin three-layer small diameter artificial vascular grafts by electrospinning

Lu¹,

Zou²,

Liao³

et al. 2022

Biomed. Mater.

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The demand for artificial vascular grafts in clinical applications is increasing, and it is urgent to design a tissue-engineered vascular graft with good biocompatibility and sufficient mechanical strength. In this study, three-layer small diameter artificial vascular grafts were constructed by electrospinning. Polycaprolactone (PCL) and collagen (COL) were used as the inner layer to provide good biocompatibility and cell adhesion, the middle layer was PCL to improve the mechanical properties, and gelatin (GEL) and PCL were used to construct the outer layer for further improving the mechanical properties and biocompatibility of the vascular grafts in the human body environment. The electrospun artificial vascular graft had good biocompatibility and mechanical properties. Its longitudinal maximum stress reached 2.63±0.12MPa, which exceeded the maximum stress that many natural blood vessels could withstand. The fibre diameter of the vascular grafts was related to the proportion of components that made up the vascular grafts. In the inner structure of the vascular grafts, the hydrophilicity of the vascular grafts was enhanced by the addition of COL to the PCL, and Human umbilical vein endothelial cells (HUVECs) adhered more easily to the vascular grafts. In particular, the cytocompatibility and proliferation of HUVECs on the scaffold with an inner structure PCL:COL = 2:1 was superior to other ratios of vascular grafts. The vascular grafts didn’t cause hemolysis of red blood cells. Thus, the bionic PCL-COL@PCL@PCL-GEL composite graft is a promising material for vascular tissue engineering.

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“…Scaffolds that are commonly invented and used today are often in the form of nanometer-scale fibers (nanofibers) because the nanofibers possess a high surface-to-volume ratio, resulting in the movement and proliferation of cells rapidly in the process of tissue formation [9][10][11] . Nanofibers are also found to be applied for blood vessel transplantation process, wound dressing, and drug delivery systems [12][13][14][15][16][17][18] .…”

mentioning

confidence: 99%

Fabrication and characterization of polycaprolactone/cellulose acetate blended nanofiber mats containing sericin and fibroin for biomedical application

Barnthip

Teeka

Kantha

et al. 2022

Sci Rep

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Polycaprolactone/cellulose acetate blended nanofiber mats containing sericin and fibroin were fabricated by electrospinning process to study the effect of sericin and fibroin on the physical and structural properties, wettability, degradability, elastic modulus, cell adhesion, and cell cytotoxicity of the electrospun nanofibers. Polycaprolactone/cellulose acetate solution was prepared with different percentage ratio of sericin and fibroin to be the running solution. Nanofibers were spun at fixed solution flow rate, flying distance, and operating voltage. The diameter of the obtained nanofibers linearly increases with the increasing of the sericin ratio. The derivative structures of polycaprolactone, cellulose acetate, sericin, and fibroin of the obtained nanofibers were confirmed by FTIR analysis. All acquired nanofibers show superhydrophilicity with adequate time of degradation for L-929 cell adhesion and growth. More elasticity is gained when the sericin ratio decreases. Moreover, all fibers containing sericin/fibroin reveal more elasticity, cell adhesion, and cell growth than that with only polycaprolactone/cellulose acetate. Greater cell adhesion and growth develop when the sericin ratio is lower. All the fabricated nanofibers are low toxic to the cells. Fibers with a mixture of sericin and fibroin at 2.5:2.5 (% w/v) are the most promising and suitable for further clinical development due to their good results in each examination. The novelty found in this study is not only making more value of the sericin, silk industrial waste, and the fibroin, but also getting the preferable biomaterials, scaffold prototype, with much greater mechanical property and slower degradation, which are required and appropriate for cell attachment and proliferation of cell generation process, compared to that obtaining from polycaprolactone/cellulose acetate or sericin/fibroin nanofibers.

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Regional anticoagulation magnetic artificial blood vessels constructed by heparin-PLCL core–shell nanofibers for rapid deployment of veno-venous bypass

Abstract: Veno-venous bypass (VVB) is necessary for maintaining hemodynamic and internal environment stabilities in complex liver surgeries. However, the current VVB strategies require systematic anticoagulation and are time-consuming, leading to unexpected...

Cited by 4 publications

References 41 publications

Enhanced hemocompatibility and rapid magnetic anastomosis of electrospun small-diameter artificial vascular grafts

Enhanced hemocompatibility and rapid magnetic anastomosis of electrospun small-diameter artificial vascular grafts

Construction of PCL-collagen@PCL@PCL-gelatin three-layer small diameter artificial vascular grafts by electrospinning

Fabrication and characterization of polycaprolactone/cellulose acetate blended nanofiber mats containing sericin and fibroin for biomedical application

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