Regional BCG-therapy of malignant melanoma: In vitro monitoring of spontaneous cytolytic activity of circulating lymphocytes

Saal, J. G.; Riethmüller, Gert; Rieber, Ernst Peter; Hadam, M.; Ehinger, H.; Schneider, W

doi:10.1007/bf00199322

Cited by 9 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the past few years, it has become clear that NK activity can be substantially augmented in vivo above normal spontaneous levels [reviewed in 5,15] by a number of agents, including viruses, interferon, interferon inducers and immunostimulants such as Corynebacterium parvurn and BCG [2,12,14,25,34]. In vitro treatment of effector cells with interferon, interferon inducers and retinoids [3] can also augment NK activity.…”

Section: Discussionmentioning

confidence: 99%

Natural cell-mediated cytotoxicity in normal human peripheral blood lymphocytes and its in vitro boosting with BCG

Mandeville

Sombo

Rocheleau

1983

Cancer Immunol Immunother

View full text Add to dashboard Cite

Natural cell-mediated cytotoxicity (NCMC) against K-562 human erythroleukemic cells was monitored in an overnight chromium release assay using normal human peripheral blood lymphocytes (PBL) as effective cells. Two hundred and ten normal individuals were tested from 3 to 24 times over a period of 3 years. The level of NCMC was shown to vary from 4% to 46% lysis at an effector-to-target cell ratio of 5/1; males had higher levels of activity than females (P less than 0.001). A group of individuals with low natural killer (NK) cell activity (below the 90% tolerance limit) was identified in replicate experiments and 60% of them were young women (ages 20--39). In vitro boosting of NK activity with Bacillus Calmette-Guérin (BCG) was also studied; overall, 56% of normal individuals responded positively to BCG. There was a significant (P less than 0.001) correlation between the unstimulated level of NCMC and the in vitro boosting with BCG, as 63% of individuals with a normal level of NK activity could be boosted as against only 19% of persons with low NK activity. We have also established the in vivo relevance of this in vitro test by determining the degree of correlation between responses to in vitro boosting with BCG and a positive or negative reaction in a hypersensitivity skin test using 5 IU of PPD (purified protein derivative of BCG). Our results indicate that NCMC is an individual trait that varies little under physiological conditions, and that the response to BCG is a characteristic property of the effector lymphocyte, depending primarily on the unstimulated level of NCMC.

show abstract

Section: Discussionmentioning

confidence: 99%

Natural cell-mediated cytotoxicity in normal human peripheral blood lymphocytes and its in vitro boosting with BCG

Mandeville

Sombo

Rocheleau

1983

Cancer Immunol Immunother

View full text Add to dashboard Cite

show abstract

“…The decline in anti-bladder tumor cytotoxicity seen with disease relapse was not due to a generalized suppression of eytotoxicity, since nonspecific cytotoxic activity was normal during this time. In a study of patients with melanoma who were treated by regional BCG therapy [47], 70% of those patients who exhibited disease progression demonstrated a progressive loss of anti-melanoma cytotoxic activity in vitro. The remaining patients in the progressive disease group maintained anti-tumor cytotoxicity.…”

Section: Serial Assessment Of Tumor-associated Immunity In Patients Wmentioning

confidence: 99%

Serial immune function testing to predict clinical disease relapse in patients with solid tumors

Braun

Harris

1983

Cancer Immunol Immunother

View full text Add to dashboard Cite

Adjuvante Chemo-Immunotherapie beim malignen Melanom

Jungi

1978

Adjuvante Zytostatische Chemotherapie

View full text Add to dashboard Cite

Regional BCG-therapy of malignant melanoma: In vitro monitoring of spontaneous cytolytic activity of circulating lymphocytes

Cited by 9 publications

References 8 publications

Natural cell-mediated cytotoxicity in normal human peripheral blood lymphocytes and its in vitro boosting with BCG

Natural cell-mediated cytotoxicity in normal human peripheral blood lymphocytes and its in vitro boosting with BCG

Serial immune function testing to predict clinical disease relapse in patients with solid tumors

Adjuvante Chemo-Immunotherapie beim malignen Melanom

Contact Info

Product

Resources

About