Regional differences in glucose transport in the mouse hippocampus

Shimada, M.; Kawamoto, Seiichi; Hirose, Yayoi; Nakanishi, M; Watanabe, Hirotoshi; Watanabe, Masaru

doi:10.1007/bf02388435

Cited by 17 publications

(16 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6-NBDG, an analogue of 2-NBDG, has been previously used to assess glucose transport in the brain in vivo (Shimada et al, 1994). Because they were concerned that diffusion of 6-NBDG after brain sectioning might blur the fluorescence images, these investigators used a freeze-dry method.…”

Section: Discussionmentioning

confidence: 99%

Fluorometric Determination of Glucose Utilization in Neurons in Vitro and in Vivo

Itoh

Abe

Takaoka

et al. 2004

J Cereb Blood Flow Metab

View full text Add to dashboard Cite

Summary:Glucose is the major energy source the adult brain utilizes under physiologic conditions. Recent findings, however, have suggested that neurons obtain most of their energy from the oxidation of extracellular lactate derived from astroglial metabolism of glucose transported into the brain from the blood. In the present studies we have used 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG), a fluorescent analogue of 2-deoxyglucose, which is often used to trace glucose utilization in neural tissues, to examine glucose metabolism in neurons in vitro and in vivo. Cultured neurons and astroglia were incubated with 2-NBDG for up to 15 minutes, and nonmetabolized 2-NBDG was washed out. We found that fluorescence intensity increased linearly with incubation time in both neurons and astroglia, indicating that both types of brain cells could utilize glucose as their energy source in vitro. To determine if the same were true in vivo, Sprague-Dawley rats were injected intravenously with a pulse bolus of 2-NBDG and decapitated 45 minutes later. Examination of brain sections demonstrated that phosphorylated 2-NBDG accumulated in hippocampal neurons and cerebellar Purkinje cells, indicating that neurons can utilize glucose in vivo as energy source.

show abstract

Section: Discussionmentioning

confidence: 99%

Fluorometric Determination of Glucose Utilization in Neurons in Vitro and in Vivo

Itoh

Abe

Takaoka

et al. 2004

J Cereb Blood Flow Metab

View full text Add to dashboard Cite

show abstract

“…We hypothesized that the non-metabolizable fluorescent glucose analog 6-deoxy- N -(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-aminoglucose (6-NBDG) (Speizer et al ., 1985) combined with high spatial resolution real-time two-photon microscopy, would be effective in addressing this issue in the living brain. Fluorescent glucose analogs have been increasingly used to study glucose transport and metabolism in many cell types as well as in astrocytes and neurons (Shimada et al ., 1994; Porras et al ., 2004; Rouach et al ., 2008). Here we examine the kinetics of 6-NBDG uptake into astrocytes and neurons in the rat barrel cortex, both during resting conditions and sensory stimulation.…”

Section: Introductionmentioning

confidence: 99%

Predominant Enhancement of Glucose Uptake in Astrocytes versus Neurons during Activation of the Somatosensory Cortex

Chuquet¹,

Quilichini²,

Nimchinsky³

et al. 2010

J. Neurosci.

186

138

View full text Add to dashboard Cite

Glucose is the primary energetic substrate of the brain, and measurements of its metabolism are the basis of major functional cerebral imaging methods. Contrary to the general view that neurons are fueled solely by glucose in proportion to their energetic needs, recent in vitro and ex vivo analyses suggest that glucose preferentially feeds astrocytes. However, the cellular fate of glucose in the intact brain has not yet been directly observed. We have used a real-time method for measuring glucose uptake in astrocytes and neurons in vivo in male rats by imaging the trafficking of the nonmetabolizable glucose analog 6-deoxy-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-aminoglucose (6-NBDG) using two-photon microscopy. During resting conditions we found that astrocytes and neurons both take up 6-NBDG at the same rate in the barrel cortex of the rat. However, during intense neuronal activity triggered by whisker stimulation, astrocytes rapidly accelerated their uptake, whereas neuronal uptake remained almost unchanged. After the stimulation period, astrocytes returned to their preactivation rates of uptake paralleling the neuronal rate of uptake. These observations suggest that glucose is taken up primarily by astrocytes, supporting the view that functional imaging experiments based on glucose analogs extraction may predominantly reflect the metabolic activity of the astrocytic network.

show abstract

“…In the previous work, however, it was found that even if the NBDG was introduced into the mouse blood vessel directly and it was killed 1 min later, no fluorescence was observed in the blood of the brain micro vessels under the low power magnification [9] . This phenomenon seems to result from the quenching of fluorescence by certain blood components.…”

mentioning

confidence: 83%

“…A fluorescent glucose analogue, 6-deoxy-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino- In the previous work, we studied glucose transport in brain tissue by using 6-deoxy-N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)-aminoglucose (NBDG) and confocal laser scanning microscope (CLSM) [9] . The fluorescent labeling compound, NBDG, has an excitation and emission maximum of 480 nm and 550 nm, respectively.…”

mentioning

confidence: 99%