Regional Distribution and Relative Frequency of Gastrointestinal Endocrine Cells in the ddN Mice: An Immunohistochemical Study

Lee, H. S.; Choi, Seong-Hun; Ku, Sae Kwang

doi:10.1111/j.1439-0264.2010.01024.x

Cited by 9 publications

(9 citation statements)

References 29 publications

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“…In the present study, SOM-14-IR cells were observed throughout the whole intestinal tract except for the large intestines of mole-rats. These results correlated with those of some rodent studies (Sandstrom & El-Salhy 2000;Ku et al 2003;Sarı et al 2007) but were quite different from those conducted on mice strains and porcupine (Ku et al 2004b(Ku et al , 2006Yaman et al 2007;Lee et al 2010).…”

Section: Duodenumsupporting

confidence: 81%

“…species and within parts of the gastrointestinal tracts. In addition, many studies have elucidated the regional distributions and relative frequencies of different endocrine cells in the gastrointestinal tracts of various vertebrates, including various species of rodents (Ku et al 2004a(Ku et al , 2004bYaman et al 2007;Lee et al 2010). The present study identified the existence and distributions of neuropeptide-secreting endocrine cells in the small and large intestines of mole-rats.…”

Section: Discussionmentioning

confidence: 99%

“…However, species-dependent variations of the distributional patterns of these IR cells have been reported. They were detected throughout the whole gastrointestinal tract of the rodent species except for cecum and rectum (Ku et al 2004b;Yaman et al 2007;Lee et al 2010), and they were detected throughout the whole gastrointestinal tract except for the colon and rectum of nude mice (Ku et al 2006). Also, these IR-cells were found in the whole gastrointestinal tract of the C57BL/6 mice except for the cecum, colon and the rectum (Ku et al 2003).…”

Section: Duodenummentioning

confidence: 95%

“…Several studies have demonstrated the regional distributions and relative frequencies of different endocrine cells in the gastrointestinal tract of various vertebrates, including several species and some of rodent species, and different kinds of these cells were described using immunohistochemical methods. In the Rodentia, the localizations of endocrine cells in the gastrointestinal tract of the rat (Sakata et al 2002;Sarı et al 2007), mice (Sandstrom & El-Salhy 2000), gerbil (Lee et al 2000), hairless mouse (Ku et al 2002), C57BL/6 mouse (Ku et al 2003), Balb/c mouse (Ku et al 2004a), ddY mouse (Ku et al 2004b), nude mice (Ku et al 2006) and ddN mice (Lee et al 2010) were demonstrated. Changes of regional distribution and relative frequency of some gastrointestinal endocrine cells with aging were also reported in mice (Sandstrom & El-Salhy 2000) and the distribution and frequency of gastrointestinal endocrine cells in various mouse species has been shown with strain-dependent differences to a certain degree (Ku et al 2002(Ku et al , 2003(Ku et al , 2004a(Ku et al , 2004b.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of neuroendocrine cells in the small and large intestines of the mole-rats (Spalax leucodon)

Bayrakdar

Yaman

Ilgün

2015

Animal Cells and Systems

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In this research, we aimed to determine the regional distribution and relative frequency of immunoreactive (IR) endocrine cells of ghrelin, glucagon, somatostatin-14 (SOM-14), neuropeptide Y (NPY), calcitonin-gene-related peptide (CGRP), insulin and galanin in small and large intestines of mole-rats (Spalax leucodon) by using immunohistochemical staining methods. For this purpose, small and large intestine regions of six adult mole-rats were used as material. In the small and large intestines of mole-rats (S. leucodon), ghrelin, glucagon, SOM-14, NPY and CGRP were identified at various frequencies, but insulin and galanin were not detected in this study. Ghrelin-IR cells were only observed in the small intestine. In addition, the existence of positive staining in the cells was decreased while passing from duodenum to ileum. Glucagon-IR cells were mainly detected in the small intestine regions and colon. SOM-14-IR cells were detected throughout the small intestines with a lesser frequency. NPY-IR cells were also detected in the small and large intestines at various frequencies but not in cecum and rectum. CGRP-IR cells were scarce in duodenum and jejunum, but their numbers increased from ileum to colon. In conclusion, the present study revealed the existence and distributions of neuropeptide-secreting endocrine cells in small and large intestines of mole-rats (S. leucodon).

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Section: Duodenumsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Duodenummentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Distribution of neuroendocrine cells in the small and large intestines of the mole-rats (Spalax leucodon)

Bayrakdar

Yaman

Ilgün

2015

Animal Cells and Systems

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“…Cells or fibres comprising >30% of the immunoreactivity for each antiserum compared with intact dermal keratinocytes or dermal tissue were regarded as positive, and the mean numbers of caspase‐3, PARP, NT and 4‐HNE‐immunoreactive epithelial cells (%, cells/100 epithelial cells) were counted using an established automated image analysis process , with some modifications. Additionally, the percentages occupied by MMP‐9 immunoreactive fibres in the dermis were calculated (%/mm 2 dermis).…”

Section: Methodsmentioning

confidence: 99%

Inhibition of UVB‐Induced Skin Damage by Exopolymers from Aureobasidium pullulans SM‐2001 in Hairless Mice

Kim

Park

Lee

et al. 2014

Basic Clin Pharma Tox

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Because antioxidants from natural sources may be an effective approach to the treatment and prevention of UV radiation-induced skin damage, the effects of purified exopolymers from Aureobasidium pullulans SM-2001 ('E-AP-SM2001') were evaluated in UVB-induced hairless mice. E-AP-SM2001 consists of 1.7% b-1,3/1,6-glucan, fibrous polysaccharides and other organic materials, such as amino acids, and mono-and di-unsaturated fatty acids (linoleic and linolenic acids) and shows antiosteoporotic and immunomodulatory effects, through antioxidant and anti-inflammatory mechanisms. Hairless mice were treated topically with vehicle, E-AP-SM2001 stock and two and four times diluted solutions once per day for 15 weeks against UVB irradiation (three times per week at 0.18 J/cm 2 ). The following parameters were evaluated in skin samples: myeloperoxidase (MPO) activity, cytokine levels [interleukin (IL)-1b and IL-10], endogenous antioxidant content (glutathione, GSH), malondialdehyde (MDA) levels, superoxide anion production; matrix metalloproteases (MMP-1, -9 and -13), GSH reductase and Nox2 (gp91phox) mRNA levels, and immunoreactivity for nitrotyrosine (NT), 4-hydroxynonenal (HNE), caspase-3, and cleaved poly (ADP-ribose) polymerase (PARP). Photoageing was induced by UVB irradiation through ROS-mediated inflammation, which was related to the depletion of endogenous antioxidants, activation of MMPs and keratinocyte apoptosis. Topical treatment with all three doses of E-AP-SM2001 and 5 nM myricetin attenuated the UV-induced depletion of GSH, activation of MMPs, production of IL-1b, the decrease in IL-10 and keratinocyte apoptosis. In this study, E-AP-SM2001 showed potent inhibitory effects against UVB-induced skin photoageing. Thus, E-AP-SM2001 may be useful as a functional ingredient in cosmetics, especially as a protective agent against UVB-induced skin photoageing.The skin is a vital organ, preserving water within the body, preventing infection and protecting against ultraviolet (UV) radiation. Skin acts as a physical barrier but also regulates the immune system and produces hormones and neurotransmitters [1,2].UVB radiation from sunlight is a major extrinsic factor in skin ageing [3]. When skin is damaged due to repeated exposure to UV radiation, photoageing occurs in skin tissue, resulting in wrinkled, lax and coarse skin with uneven pigmentation and brown spots [4,5]. UV radiation-induced skin damage is characterized by histological changes, including damage to collagen fibres, excessive deposition of abnormal elastic fibres and increased levels of glycosaminoglycans [4,6]. These alterations were found in the dermal connective tissues of photoaged skin in histological and ultrastructural studies [4,7]. Chronic exposure of the skin to UV radiation, particularly to UVB (290-320 nm), has been shown to induce clinical and histological damage as a result of simultaneous skin destruction and repair [8]. UVB light is absorbed mainly in the epidermis, which consists primarily of keratinocytes [9]. Naturally aged skin is ...

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Microanalysis of the stomach of southern white‐breasted hedgehog (Erinaceus concolor): Histological, histochemical, immunohistochemical, and scanning electron microscopic studies

Almasi

Goodarzi

2022

Microscopy Res & Technique

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This study was designed to provide more detailed knowledge on the stomach histochemistry and immunohistochemistry in the southern white-breasted hedgehog (Erinaceus concolor). Two animals were used in the present work. Periodic acid Schiff's (PAS) and Alcian blue were used for histochemical purposes. SOX9, gastrin, serotonin, and glucagon markers were traced immunohistochemically. The mucosa was extremely folded in the fundus with numerous opening of glands. The body and pylorus mucosa were almost smooth and equipped with gastric gland openings. A simple columnar epithelium covered the stomach entirely. Cardiac glands region was mucus secreting with both positive and negative reactions to PAS. Fundic mucosa was contained cardiac glands near to the cardia, and toward the body it was divided into the light and dark zones. These zones and body contained proper gastric gland, which constituted of parietal, chief, and mucous neck cells. These glands contained PASpositive cells on their basal portions. The pyloric glands were mucus secreting but negative for PAS. All gastric glands were Alcian blue-negative, but epithelium showed moderate reaction especially in the pylorus. SOX and gastrin were express highly in the body and fundus. The expression of serotonin and glucagon was rare. Comparatively, some similarities between the stomach of hedgehog and dog can be assumed.The present findings provide additional information concerning the histochemical characteristics and endocrine cells distribution in the stomach of the southern whitebreasted hedgehog (Erinaceus concolor). Further detailed studies are required to enhance the current knowledge on histophysiology of the digestive system in this species as a pet and exotic animal. Highlights• The stomach was simple glandular type.• The fundus was divided into light and dark zones similar to the dog.• The proper gastric glands were periodic acid Schiff's positive at their basal parts.

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Regional Distribution and Relative Frequency of Gastrointestinal Endocrine Cells in the ddN Mice: An Immunohistochemical Study

Cited by 9 publications

References 29 publications

Distribution of neuroendocrine cells in the small and large intestines of the mole-rats (Spalax leucodon)

Distribution of neuroendocrine cells in the small and large intestines of the mole-rats (Spalax leucodon)

Inhibition of UVB‐Induced Skin Damage by Exopolymers from Aureobasidium pullulans SM‐2001 in Hairless Mice

Microanalysis of the stomach of southern white‐breasted hedgehog (Erinaceus concolor): Histological, histochemical, immunohistochemical, and scanning electron microscopic studies

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