Regional distribution of dopamine, 5-hydroxytryptamine, and noradrenaline in the rat vas deferens

Celuch, Stella Maris; Sloley, B.D.

doi:10.1007/bf00970770

Cited by 14 publications

(11 citation statements)

References 22 publications

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“…Fuenmayor et al [20] and Celuch and Sloley [21] described the presence and release of serotonin, dopamine and noradrenalin (NA) from rat vas deferens. It is conceivable that protection from hypothermia in SMAC cells is dependent on the cellular uptake of serotonin, in view of the failure of its protection in the presence of an SSRI and the unchanged effectiveness of serotonin in the presence of the non-selective 5-HT 2 receptor blocker ketanserin.…”

Section: Discussionmentioning

confidence: 99%

Serotonin and Dopamine Protect from Hypothermia/Rewarming Damage through the CBS/ H2S Pathway

Talaei

Bouma

Graaf³

et al. 2011

PLoS ONE

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Biogenic amines have been demonstrated to protect cells from apoptotic cell death. Herein we show for the first time that serotonin and dopamine increase H2S production by the endogenous enzyme cystathionine-β-synthase (CBS) and protect cells against hypothermia/rewarming induced reactive oxygen species (ROS) formation and apoptosis. Treatment with both compounds doubled CBS expression through mammalian target of rapamycin (mTOR) and increased H2S production in cultured rat smooth muscle cells. In addition, serotonin and dopamine treatment significantly reduced ROS formation. The beneficial effect of both compounds was minimized by inhibition of their re-uptake and by pharmacological inhibition of CBS or its down-regulation by siRNA. Exogenous administration of H2S and activation of CBS by Prydoxal 5′-phosphate also protected cells from hypothermic damage. Finally, serotonin and dopamine pretreatment of rat lung, kidney, liver and heart prior to 24 h of hypothermia at 3°C followed by 30 min of rewarming at 37°C upregulated the expression of CBS, strongly reduced caspase activity and maintained the physiological pH compared to untreated tissues. Thus, dopamine and serotonin protect cells against hypothermia/rewarming induced damage by increasing H2S production mediated through CBS. Our data identify a novel molecular link between biogenic amines and the H2S pathway, which may profoundly affect our understanding of the biological effects of monoamine neurotransmitters.

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Section: Discussionmentioning

confidence: 99%

Serotonin and Dopamine Protect from Hypothermia/Rewarming Damage through the CBS/ H2S Pathway

Talaei

Bouma

Graaf³

et al. 2011

PLoS ONE

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“…In the epididymis, sperm achieve the capacity to move (Robaire & Hermo 1988) and undergo changes in the cytoplasmic membrane (Shalgi et al 1989). Serotonin is present in the epididymis (Leung et al 1999) and vas deferens (Celuch & Sloley 1988), but its function is not known.…”

Section: Discussionmentioning

confidence: 99%

Serotoninergic system blockage in the prepubertal rat inhibits spermatogenesis development

Martínez¹,

Ayala²,

Marin³

et al. 2005

Reproduction

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The stimulatory and inhibitory role of serotonin in gonadotropin secretion and in the onset of puberty in the male rat has been previously described, but its role in the establishment of spermatogenesis is not known. The aim of this study was to investigate the effects of serotoninergic inhibition by p-chloroamphetamine (pCA) on the prepubertal-to-adult stage of the rat reproductive system. Hypothalamic serotonin, gonadotropins and sex steroid hormone concentrations were measured, and a histopathological analysis of seminiferous epithelium was carried out on animals treated with pCA from day 30 and killed at 45 or 65 days of age. The pCA treatment significantly reduced the hypothalamic levels of serotonin and its metabolite (5-hydroxyindole-3-acetic acid). This inhibition did not affect the sex steroid hormone or LH concentrations, but rather it induced an increase in FSH concentration in animals of both ages. Spermatogenesis was impaired by pCA treatment. Disruption of seminiferous epithelium and the death of numerous germ cells were observed. Sperm produced by pCA-treated animals was of poor quality and appeared in small quantities. Apparently, serotonin depletion did not affect communication between the hypothalamus and the pituitary, but the FSH increase could have been related to alterations in the seminiferous epithelium effects. The seminiferous epithelium cycle was altered in rats killed at both 45 and 65 days of age, because at each age of killing the distribution of spermatogenesis stages was different. Germ cell apoptosis did not appear to be related to changes in the FSH concentrations, but other factors produced during spermatogenesis could have been involved in this induction. This study showed that serotonin was necessary for the development of normal spermatogenesis in prepubertal rats. Reproduction (2005) 129 717-727

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“…Previous studies demonstrated that dopamine (DA) and noradrenaline (NE) coexist in the same sympathetic neurons of the rat vas deferens. Concentrations of DA and NE are maximal near the prostatic end and decrease toward the epididymis [6]. Also, dopamine-betahydroxylase (DBH), an enzyme that converts DA to NE, has been shown to be present in the NE-and peptide-containing nerves in the epididymis of the boar [7,8].…”

Section: Introductionmentioning

confidence: 99%

Stimulating Effects of Dopamine on Chloride Transport Across the Rat Caudal Epididymal Epithelium in Culture1

Zuo

Ruan

et al. 2009

Biology of Reproduction

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The present study investigated the effects of dopamine on chloride transport across cultured rat caudal epididymal epithelium. The results showed that dopamine induced a biphasic short-circuit current (Isc) in a concentration-dependent manner. The dopamine-induced response consisted of an initial rapid spike followed by a sustained phase. The alpha and beta adrenoreceptor inhibitors, phentolamine and propranolol, inhibited the initial spike and the sustained phase, respectively, suggesting a contribution of adrenergic receptors. The response was almost abolished by removing the extracellular Cl-, suggesting that the dopamine-induced short-circuit current is primarily a Cl- current. The response was inhibited by the apical Cl- channel blocker, diphenylamine-dicarboxylic acid, and the Ca2+-activated Cl- channel blocker, disulfonic acid stilbene, indicating that Cl- may pass through two types of Cl- channels on the apical side. Preloading monolayers with the intracellular Ca2+ chelator BAPTA/AM abolished the initial spike and greatly reduced the second phase in the Isc response to dopamine. Pretreating the monolayers with an adenylate cyclase inhibitor, MDL12330A, inhibited all of the second Isc response and part of the initial spike. Also, characteristics of the Cl- currents induced by dopamine were observed in whole-cell patch-clamp recording. The increases of intracellular cAMP and Ca2+ induced by dopamine were also measured. The results suggest that extracellular dopamine activates Ca2+-dependent and cAMP-dependent regulatory pathways, leading to activation of both Ca2+-dependent and cAMP-dependent Cl- conductances in epididymal epithelial cells.

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Regional distribution of dopamine, 5-hydroxytryptamine, and noradrenaline in the rat vas deferens

Cited by 14 publications

References 22 publications

Serotonin and Dopamine Protect from Hypothermia/Rewarming Damage through the CBS/ H2S Pathway

Serotonin and Dopamine Protect from Hypothermia/Rewarming Damage through the CBS/ H2S Pathway

Serotoninergic system blockage in the prepubertal rat inhibits spermatogenesis development

Stimulating Effects of Dopamine on Chloride Transport Across the Rat Caudal Epididymal Epithelium in Culture1

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