Regional Mapping Panels for Chromosomes 3, 4, 5, 11, 15, 17, 18, and X

Leonard, Jay; Drwinga, Helen L.; Kim, C.H.; Toji, Lorraine; Bender, Patrick K.; Mulivor, R.A.; Beck, Jeanne C.

doi:10.1006/geno.1997.5047

Cited by 3 publications

(1 citation statement)

References 39 publications

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“…The MYLK 3 and MYLKP sequences (referred to henceforth as MLCK and pMLCK, which are the usual literature names) were subregionally mapped on chromosome 3 by PCR-typing a chromosome-3 mapping panel consisting of cell line DNAs containing different portions of human chromosome 3 (Coriell Institute, Camden, NJ, (Leonard et al, 1997)). Twenty-five-microliter PCRs were performed on 40 ng of DNA using the primer set MLCK3ar (5Ј TGA ACT TGG TGG TCT TGA GG 3Ј) and MLCK3bf (5Ј ATG GAT CTC CGT GCC AAC C 3Ј) in a buffer containing 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl 2 , 0.001% (w/v) gelatin, 0.2 mM each deoxyribonucleotide, 1 M each primer, and 0.625 U of Taq DNA polymerase (Takara Shuzo Co., Ltd.).…”

Section: Chromosomal Assignmentsmentioning

confidence: 99%

A Genomic Region Encompassing a Cluster of Olfactory Receptor Genes and a Myosin Light Chain Kinase (MYLK) Gene Is Duplicated on Human Chromosome Regions 3q13–q21 and 3p13

et al. 1999

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Section: Chromosomal Assignmentsmentioning

confidence: 99%

A Genomic Region Encompassing a Cluster of Olfactory Receptor Genes and a Myosin Light Chain Kinase (MYLK) Gene Is Duplicated on Human Chromosome Regions 3q13–q21 and 3p13

et al. 1999

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The Linked Human Elongation Initiation Factor 4A1 (EIF4A1) and CD68 Genes Map to Chromosome 17p13

Jones

Quinn

See³

et al. 1998

Genomics

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Tanabe

Nakagawa

Minegishi

et al. 2000

Chromosome Research

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The human monochromosome hybrid cell panel in the Japanese Collection of Research Bioresources (JCRB) consists of 23 mouse cell clones, each containing a different human chromosome (the Y chromosome is not yet included). The panel is currently distributed by the Human Science Research Resources Bank (HSRRB) in Osaka. In order to determine the state of the human chromosomes and to supply the information to investigators, we characterized the cells by fluorescence in-situ hybridization (FISH) with corresponding human chromosome-specific painting probes, and, in part, by reverse FISH with the hybrid total DNA hybridized onto human metaphase spreads. Here, we report the frequency of intact human chromosomes maintained in each hybrid and the retained subregions of corresponding human chromosomes with relative frequencies estimated by fluorescent intensity. We used specific painted patterns to classify each hybrid into tentative types with their frequencies showing the nature of each hybrid and the state of rearrangements. This characterization will provide valuable information to investigators using the panel.

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Regional Mapping Panels for Chromosomes 3, 4, 5, 11, 15, 17, 18, and X

Cited by 3 publications

References 39 publications

A Genomic Region Encompassing a Cluster of Olfactory Receptor Genes and a Myosin Light Chain Kinase (MYLK) Gene Is Duplicated on Human Chromosome Regions 3q13–q21 and 3p13

A Genomic Region Encompassing a Cluster of Olfactory Receptor Genes and a Myosin Light Chain Kinase (MYLK) Gene Is Duplicated on Human Chromosome Regions 3q13–q21 and 3p13

The Linked Human Elongation Initiation Factor 4A1 (EIF4A1) and CD68 Genes Map to Chromosome 17p13

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