Regional patterns of microsatellite variation in Mytilus galloprovincialis from the Iberian Peninsula

Diz, Angel P.; Presa, Pablo

doi:10.1007/s00227-008-0921-3

Cited by 62 publications

(68 citation statements)

References 49 publications

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“…Indeed, our pairwise F ST values were approximately 10 times higher than those reported in 2 other microsatellite-based analyses of population genetic structure in Mytilus (M. galloprovincialis; Diz & Presa 2008). This anomaly may reflect the non-equilibrium nature of our sampled hybrid zone populations, and possible resulting Wahlund or related effects that inflate our F ST estimates.…”

Section: Spatial Genetic Structurecontrasting

confidence: 59%

“…However, since micro- satellite DNA-based markers often exhibit substantial heterozygote deficits in marine bivalves (e.g. Vadopalas et al 2004, Kenchington et al 2006, Cassista & Hart 2007, Diz & Presa 2008, as do our Mytilus microsatellite markers, we cannot rule out the possibility that the genetic structure reported here may be biased by limitations in our markers. Although the abundance of non-native genotypes on southern VI varies over time at individual sites, the region-level abundance did not (Fig.…”

Section: Spatial Genetic Structurementioning

confidence: 87%

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Marine landscape shapes hybrid zone in a broadcast spawning bivalve: introgression and genetic structure in Canadian west coast Mytilus

Shields¹,

Heath²,

Heath³

2010

Mar. Ecol. Prog. Ser.

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Adult marine mussels are sessile, but their highly dispersive planktonic larval stage plays a critical role in shaping population structure. However, shoreline geography and oceanographic currents can modify the dispersal pattern of pelagic larvae. On Vancouver Island (VI), British Columbia, 3 species of blue mussels (native Mytilus trossulus and introduced M. galloprovincialis and M. edulis) form a localized hybrid zone. Here we genetically mapped the distribution of Mytilus species and populations along VI and the surrounding islands. Using diagnostic species markers and microsatellite loci, we estimated the extent of the Mytilus hybrid zone on VI and measured population differentiation among the observed sites in 2005 and 2006. We predicted that the distribution of non-native genotypes would be mirrored by the microsatellite allelic patterns, which correspond to oceanographic features that reflect barriers to gene flow in the Strait of Georgia. Generally, non-native genotypes were restricted to southern VI and strong microsatellite population structure was detected. The distribution of non-native genotypes reflected patterns of microsatellite allele frequency in the Strait of Georgia. Using a landscape genetics approach, we identified 2 genetic discontinuities, which correspond to oceanographic and hydrographic features of the Strait of Georgia. Thus, physical dispersal barriers likely limit the spread of the VI Mytilus hybrid zone; however, additional biological barriers to dispersal must also exist. The VI Mytilus hybrid zone provides an excellent example of complex dispersal patterns in a non-equilibrium system.

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Section: Spatial Genetic Structurecontrasting

confidence: 59%

Section: Spatial Genetic Structurementioning

confidence: 87%

Marine landscape shapes hybrid zone in a broadcast spawning bivalve: introgression and genetic structure in Canadian west coast Mytilus

Shields¹,

Heath²,

Heath³

2010

Mar. Ecol. Prog. Ser.

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“…Deviations from HWE have been previously reported in MS marker analyses of M. coruscus (Xu et al, 2010), M. galloprovincia (Diz and Presa, 2008), and other mollusk species (Hedgecock et al, 2004;Li et al, 2006;Panova et al, 2008). These deviations might arise because of one or a combination of factors, such as the substructure of samples caused by the pooling of samples from several different sites, inbreeding, or the presence of null alleles (Zouros and Foltz, 1984).…”

Section: Discussionmentioning

confidence: 84%

“…In comparison, the second cluster contained different populations also from the western (Mg-TA), eastern (Mg-JMJ), and southern (Mg-JD) coasts. A genetic split of M. galloprovincialis populations has been documented between populations in the Atlantic Ocean and the Mediterranean Sea, with an interbasin F ST estimate of 0.0306 (Diz and Presa, 2008). Although the relationship in the genetic separation of M. galloprovincialis in the seas around Europe and the 3 seas around the Korean Peninsula has not been elucidated, the results of this study (i.e., cluster analysis) show that more than 2 populations from different origins have been introduced to the Korean Peninsula.…”

Section: Discussionmentioning

confidence: 99%

Genetic characterization of Mytilus coruscus and M. galloprovincialis using microsatellite markers

Kang

Lee²,

Noh³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Korean (hard-shelled) mussels (Mytilus coruscus) are an economically important endemic marine bivalve mollusk of Korea; yet, the population has rapidly declined because of overharvesting and habitat competition from the invasive Mytilus galloprovincialis species. The population structures of M. coruscus and M. galloprovincialis were analyzed by next-generation sequencing using 5 microsatellite markers specifically developed for M. coruscus. M. galloprovincialis had an average of 5.4 alleles per locus (range = 2-10), with an average allelic richness of 4.9 per locus (range = 2.0-9.3). M. coruscus had an average of 5.7 alleles per locus (range = 2-13), with an average allelic richness of 5.2 per locus (range = 2.0-11.9). Excessive homozygosity was observed at 3 loci, which was assumed to be due to the presence of null alleles at these loci. Pairwise multilocus F ST estimates showed that the M. coruscus and M. galloprovincialis populations were clearly separated. Six populations of M. galloprovincialis from the western, eastern, and southern coast of Korea formed 2 separate clusters, indicating that more than 2 populations of M. galloprovincialis have been introduced to the Korean Peninsula. Hybrids between M. coruscus 5495 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (4): 5494-5505 (2013) Genetic characterization of Mytilus mussels and M. galloprovincialis were not identified, probably because of genetic differences or different habitat preferences. Further genetic information is required to perform selective breeding, population management, and restoration of M. coruscus.

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“…They have proven to be valuable for research in different areas, including genetic mapping, individual identification, parentage assignment, genetic dissection of complex traits and population and conservation genetics (Chistiakov et al 2006). In the field of population genetics and management of marine resources their use has revealed the existence of genetic structuring in species previously thought to be homogeneous over large geographical ranges (Jørgensen et al 2005;Was et al 2008) and differentiation at fine-scale in others (Cabranes et al 2008;Diz and Presa 2008). The principal drawback of microsatellites is that usually they must be isolated and characterized in each species given that the flanking sequences are not often conserved across species.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of microsatellite markers in the queen scallopAequipecten opercularisand their application to a population genetic study

Arias

Freire

Méndez

et al. 2010

Aquat. Living Resour.

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-Microsatellites are one of the most popular markers in genetic studies but typically they need to be isolated and characterized de novo for each species. In this work, a genomic library enriched for a trinucleotide motif was constructed to identify polymorphic microsatellite loci in Aequipecten opercularis, a scallop species commercially fished in Europe, and to examine the level of genetic variation and genetic differentiation in samples from Spain and Northern Ireland. Sequencing of 83 clones led to the identification of 30 microsatellite-containing sequences which showed often other repeated sequences. Five microsatellite loci were successfully amplified and found polymorphic. The number of alleles and the expected heterozygosity per locus ranged from 9 to 86 and 0.341 to 0.927, respectively, all localities showing similar levels of genetic variation (allelic richness, 13.164-15.487; expected heterozygosity, 0.527-0.638). Discrepancies in genotype proportions from Hardy-Weinberg equilibrium were observed in 11 out of 25 locality-locus combinations, a heterozygote deficiency occurring in all cases probably due to null alleles. Significant genetic differentiation was detected among A. opercularis from Northern Ireland, Fuengirola (southern Spain) and the homogeneous samples from northwest Spain. Isolation by distance was the most likely hypothesis to explain the differentiation detected. (richesse allélique : 13,164-15,487 ; hétérozygosité attendue : 0,527-0,638). Des différences dans les proportions de génotype sont observées par rapport à l'équilibre de Hardy-Weinberg dans 11 des 25 combinaisons « sites géographiques-locus », une déficience hétérozygote est détectée dans tous les cas, probablement due aux allèles nuls. Une différentiation génétique significative est détectée parmi les échantillons de A. opercularis provenant d'Irlande du Nord, de Fuengirola (sud de l'Espagne), et des échantillons homogènes du nord-ouest de l'Espagne. L'isolation due à la distance est l'hypothèse la plus probable pour expliquer ces différences détectées.

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Regional patterns of microsatellite variation in Mytilus galloprovincialis from the Iberian Peninsula

Cited by 62 publications

References 49 publications

Marine landscape shapes hybrid zone in a broadcast spawning bivalve: introgression and genetic structure in Canadian west coast Mytilus

Marine landscape shapes hybrid zone in a broadcast spawning bivalve: introgression and genetic structure in Canadian west coast Mytilus

Genetic characterization of Mytilus coruscus and M. galloprovincialis using microsatellite markers

Isolation and characterization of microsatellite markers in the queen scallopAequipecten opercularisand their application to a population genetic study

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