Regional Pleural Filtration and Absorption Measured by Fluorescent Tracers in Rabbits

Wang, P. M.; Lai-Fook, S. J.

doi:10.1007/pl00007648

Cited by 14 publications

(14 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tracing marker administered into the peritoneal cavity was found to travel via the diaphragm to the sternal lymphatics located in the ventral inner-wall of the thoracic cavity. Subsequently, the same drainage pattern was observed in many animals [4,7,9]. This lymphatic drainage route may be clinically useful in predicting the course of intraperitoneal cancer dissemination.…”

supporting

confidence: 63%

“…This may be due to the pressure of the liver on the right surface of the diaphragm. The uptake of lymph from the peritoneal side of the diaphragm's surface depends on the mesothelial cells of the diaphragm surface [1,2,5,8,9]. There may be more stomata located in the mesothelium on the surfaces with organ contact than on surfaces with no organ contact.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Time Course of Lymph Drainage from the Peritoneal Cavity in Beagle Dogs

Shibata

Hiramatsu

Kaseda

et al. 2006

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. Lymph drainage routes from the abdominal and pelvic cavities in beagle dogs were observed serially by following the time course of India ink administered intraperitoneally. Four systems of lymph drainage routes from the peritoneal cavity were observed in this study. The earliest drainage returned to the cranial mediastinal lymph nodes via the sternal lymph vessels; subsequently, the sternal lymph nodes located along the internal thoracic artery became involved. Then, a drainage route via the lymph vessel along the left vagus nerve was observed. The final drainage route flowed into the lateral lymph vessel through the thoracic duct located on the vertebra. These results show that India ink is absorbed from the peritoneal cavity, and that the lymph drainage first flows mainly towards the cranial mediastinal lymph nodes through the ventral lymphatic channels. Our serial observations suggest that, over time, the lymph drainage routes changed from the ventral abdominal to the dorsal thoracic lymphatic channels in the thorax. KEY WORDS: beagle dog, lymph drainage, peritoneal cavity.J. Vet. Med. Sci. 68(11): 1143-1147, 2006 The lymphatic system establishes a lymphatic circulation and transports lymph containing lipid droplets, immunocytes, and tumor cells. Ultimately, lymphatic drainage routes flow into the systemic circulation. However, parts of the lymphatic system are located in serous cavities. These lymphatic routes have yet to be adequately clarified. Previously, a limited amount of information about the lymphatic drainage from the abdominal cavity was collected by examining the route that drains lymph from the canine peritoneal cavity [6]. Tracing marker administered into the peritoneal cavity was found to travel via the diaphragm to the sternal lymphatics located in the ventral inner-wall of the thoracic cavity. Subsequently, the same drainage pattern was observed in many animals [4,7,9]. This lymphatic drainage route may be clinically useful in predicting the course of intraperitoneal cancer dissemination. The present paper provides a detailed description of lymph drainage routes over time following the intraperitoneal administration of India ink in beagle dogs. MATERIALS AND METHODSThree adult male beagle dogs (body weight, 8.5 kg) and one adult female beagle dog (body weight, 6.7 kg) were used for this experiment.Using a fine needle, the male dogs were all injected intraperitoneally with warm India ink dissolved in physiological saline at a dose of 25 ml per kg body weight; the India ink was injected into the pelvic cavity via the rectum in the female dog. The male dogs were sacrificed by an overdose of Nembutal (0.2 ml/kg) at 30 min, 60 min, and 2.5 hr after ink injection, respectively. The female dog was sacrificed at 2.5 hr. A fixative solution [24 (ethylene glycol): 8 (phenol): 1 (formaldehyde solution): 16 (methanol)] was immediately injected intraperitoneally and intrapelvically after the animals were sacrificed. The peritoneal and pelvic cavities were dissected from the carcasses, and these ...

show abstract

supporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

The Time Course of Lymph Drainage from the Peritoneal Cavity in Beagle Dogs

Shibata

Hiramatsu

Kaseda

et al. 2006

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…Of these factors, the lymphatic drainage system via lymphatic stomata likely plays an important role (30)(31)(32)(33)(34), which may contribute up to approximately 75-80% of the fluid absorption rate in the abdominal and pleural cavities (33,35).…”

Section: Serosal Membranes Lymphatic Stomata and Coelomic Fluid Draimentioning

confidence: 99%

The role of the lymphatic system in rabbit models for cancer metastasis research: a perspective from comparative anatomy

Oshiro

2014

Okajimas Folia Anat. Jpn.

View full text Add to dashboard Cite

Summary:The elucidation of the pathogenesis of human diseases requires increasingly relevant and rigorous animal models. Therefore, investigators must select an appropriate mammalian model. Mice and rats are indispensable in the understanding of the mechanisms of human diseases, but other non-rodent mammals are required in certain situations. The rabbit is one such species. The rabbit exhibits greater biological similarities to humans than the mouse or rat, and the rabbit VX2 allograft cancer model has been used in a broad range of oncological studies, such as stromal responses, metastatic behaviors and therapeutic effects. Cancer cells in this model proliferate in a host rabbit that maintains a natural immunity, which makes this model attractive and unique. However, these examples constitute only a small number of advantages of a rabbit model. Numerous reports suggest that the rabbit is an attractive cancer-bearing animal model for the study of cancer metastasis and the lymphatic system. I briefly review the relevant medical literature and compare the rabbit lymphatic system with mice, rats and humans.

show abstract

“…1,2 Of these factors, the lymphatic drainage system via the lymphatic stomata is considered to play an important role, contributing up to approximately 75%-80% of the fluid absorption rate in the serosal cavity. [1][2][3][4][5][6] Lymphatic stomata are small openings that enable direct communication between the lymphatic lumen and the serosal cavity. 7 Since apertures in the diaphragmatic peritoneum were first described, 8 numerous investigators have confirmed the existence of lymphatic stomata not only in the peritoneum but also in the pleura of various types of mammals, such as rabbits, 9 rats, [9][10][11] sheep, 12,13 monkeys, 14,15 golden hamsters, 16 and mice.…”

Section: Introductionmentioning

confidence: 99%

Lymphatic Stomata in the Adult Human Pulmonary Ligament

Oshiro

Miura

Iobe

et al. 2015

Lymphatic Research and Biology

View full text Add to dashboard Cite

Background: Lymphatic stomata are small lymphatic openings in the serosal membrane that communicate with the serosal cavity. Although these stomata have primarily been studied in experimental mammals, little is known concerning the presence and properties of lymphatic stomata in the adult human pleura. Thus, adult human pleurae were examined for the presence or absence of lymphatic stomata. Methods and Results: A total of 26 pulmonary ligaments (13 left and 13 right) were obtained from 15 adult human autopsy cases and examined using electron and light microscopy. The microscopic studies revealed the presence of apertures fringed with D2-40-positive, CD31-positive, and cytokeratin-negative endothelial cells directly communicating with submesothelial lymphatics in all of the pulmonary ligaments. The apertures' sizes and densities varied from case to case according to the serial tissue section. The medians of these aperture sizes ranged from 2.25 to 8.75 lm in the left pulmonary ligaments and from 2.50 to 12.50 lm in the right pulmonary ligaments. The densities of the apertures ranged from 2 to 9 per mm 2 in the left pulmonary ligaments and from 2 to 18 per mm 2 in the right pulmonary ligaments. However, no significant differences were found regarding the aperture size ( p = 0.359) and density ( p = 0.438) between the left and the right pulmonary ligaments. Conclusions: Our study revealed that apertures exhibit structural adequacy as lymphatic stomata on the surface of the pulmonary ligament, thereby providing evidence that lymphatic stomata are present in the adult human pleura.

show abstract

Regional Pleural Filtration and Absorption Measured by Fluorescent Tracers in Rabbits

Cited by 14 publications

References 26 publications

The Time Course of Lymph Drainage from the Peritoneal Cavity in Beagle Dogs

The Time Course of Lymph Drainage from the Peritoneal Cavity in Beagle Dogs

The role of the lymphatic system in rabbit models for cancer metastasis research: a perspective from comparative anatomy

Lymphatic Stomata in the Adult Human Pulmonary Ligament

Contact Info

Product

Resources

About