Regiospecific oxidoreductions catalyzed by a new Pseudomonas paucimobilis hydroxysteroid dehydrogenase

7α- and 12α-Hydroxysteroid dehydrogenases from Acinetobacter calcoaceticus lwoffii: a new integrated chemo-enzymatic route to ursodeoxycholic acid

“…For a long time our research group has been interested in the synthesis [26,27] and biotransformation of bile acids [28][29][30].…”

Exploitation of the alcohol dehydrogenase‐acetone NADP‐regeneration system for the enzymatic preparative‐scale production of 12‐ketochenodeoxycholic acid

“…Among the various dehydrogenases that have found application in the area of fine and pharmaceutical chemistry, bacterial hydroxysteroid dehydrogenases have been employed for the selective modification of neutral steroids, bile acid, and other steroid derivatives (Anelli et al, 2002;Bianchini et al, 1999;Medici et al, 2002;Secundo et al, 2003). (2) is an important intermediate in the synthesis of ursodeoxycholic acid, a drug used in cholesterol gall-stones dissolution therapy and in the treatment of the autoimmune hepatopathy, known as primary sclerosis cholangitis (PSC) (Beuers, 2005;Festi et al, 1990).…”

Optimization and Impurity Control Strategy for Lithocholic Acid Production Using Commercially Plant-Sourced Bisnoralcohol

“…In general, a 100% isomerization product is undoubtedly the most desirable outcome for drug synthesis, 3α-hydroxysteroid dehydrogenase (3α-HSDH) from Comamonas testosteroni stereoselectively reduced prochiral ketones to chiral secondary alcohols using reduced nicotinamide adenine dinucleotide (NADH) as a cofactor, which was regenerated with a recycling system comprising glucose dehydrogenase (GDH) and glucose. The structure and reaction mechanism of the 3α-HSDH is clear. − 3α-HSDH and GDH were heterologously expressed in Escherichia coli BL21 (DE3). Based on the stability of the enzyme and the cost of subsequent industrialization, the 3α-HSDH and the GDH used were both high-density cultured E.…”