Registration of 17 upland cotton germplasm lines with improved resistance to Fusarium wilt race 4 and good fiber quality

Ulloa, Mauricio; Hutmacher, Robert B.; Zhang, Jinfa; Schramm, TariLee; Roberts, Philip A.; Ellis, Margaret L.; Dever, Jane K.; Wheeler, Terry A.; Witt, Travis W.; Sanogo, Soum; Hague, Steve; Keely, Mark P.; Arce, Joel; Ángeles, Jorge; Hake, Kater; Payton, Paxton

doi:10.1002/plr2.20258

Cited by 8 publications

(7 citation statements)

References 34 publications

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“…Breeding programs have successfully developed FOV4-resistant cotton germplasm genome [6,11,12]. However, little is known about the molecular mechanisms underlying FOV4 resistance in this crop.…”

Section: Discussionmentioning

confidence: 99%

Enhanced phenylpropanoid metabolism underlies resistance to Fusarium oxysporum vasinfectum f. sp. race 4 infection in the cotton cultivar Pima-S6 (Gossypium barbadense L.)

Ojeda-Rivera,

Ulloa,

Najera-Gonzalez

et al. 2023

Preprint

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Background Fusarium oxysporum f. sp. vasinfectum (FOV) race 4 (FOV4) is a highly pathogenic soil-borne fungus responsible for Fusarium wilt in cotton (Gossypium spp.) and represents a continuing threat to cotton production in the southwest states of the U.S., including California, New Mexico, and Texas. Even though the Pima (G. barbadense L.) cotton gene pool shows more susceptibility to this pathogen than Upland (G. hirsutum L.) cotton, fortunately, some Pima cultivars present resistance to FOV4 infection, like Pima-S6. To gain insights into the mechanism underlying FOV4 resistance, we performed comparative transcriptional and metabolomic profiling of FOV4-resistant Pima-S6 and FOV4-susceptible Pima S-7 and Pima 3–79 cotton. Results Comparative analysis of transcriptional FOV4 infection responses on these genotypes indicated a differential response between resistant Pima-S6 and susceptible Pima S-7 and 3–79. GO-enriched analysis found clusters related to various biological processes, including transmembrane transport, glycoside hydrolysis, metabolism of small molecules, and phenylpropanoid metabolism. Arabidopsis ortholog genes comparison with categories significantly enriched in Pima-S6 resulted in multiple categories clustered to similar biological processes such as the metabolism of small molecules, regulation of enzymatic activity, diterpenoid biosynthetic processes, and phytohormone signaling-related processes, being phenylpropanoid biosynthesis and metabolism highly enriched. These results correlated with the accumulation of secondary metabolites in Pima-S6 roots, specifically esculetin, a coumarin that inhibits the growth of Fusarium. Transcriptomic profiling enabled the identification of candidate genes potentially involved in the resistance mechanism of FOV4-resistance in protecting the Pima-S6 cotton cultivar from this pathogenic fungus. Some genes of this pathway were found to be in chromosome-QTL regions linked with resistance to FOV4. Conclusions Our results highlight an essential role for the phenylpropanoid synthesis pathway in FOV4 resistance and create opportunities for gene discovery and SNP development for marker-associated selection (MAS) for breeding for FOV4 resistance.

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Section: Discussionmentioning

confidence: 99%

Enhanced phenylpropanoid metabolism underlies resistance to Fusarium oxysporum vasinfectum f. sp. race 4 infection in the cotton cultivar Pima-S6 (Gossypium barbadense L.)

Ojeda-Rivera,

Ulloa,

Najera-Gonzalez

et al. 2023

Preprint

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“…2021; Ulloa et al, 2022;Zhang, Abdelraheem, Zhu, Wheeler, Dever, Nichols et al, 2021;Zhu, Abdelraheem, Lujan et al, 2021;Zhu, Abdelraheem, Wheeler et al, 2021), although its responses to FOV4 infections were described as tolerant or moderately tolerant in literature (Diaz et al, 2021;Ulloa et al, 2022Ulloa et al, , 2023. Other resistant Upland cultivars and breeding lines were recently identified (e.g., Abdelraheem et al, 2020;Ulloa et al, 2020Ulloa et al, , 2023Zhang et al, 2016;Zhang, Idowu et al, 2020).…”

Section: Crop Sciencementioning

confidence: 99%

“…FM 2334GLT is a commercial transgenic cultivar with FOV4 resistance within Upland cotton ( G. hirsutum L.) in the United States because it consistently showed reduced disease severity caused by FOV4 relative to susceptible plants (Diaz et al., 2021; Ulloa et al., 2022; Zhang, Abdelraheem, Zhu, Wheeler, Dever, Nichols et al., 2021; Zhang, Zhu, Abdelraheem et al., 2022; Zhu, Abdelraheem, Lujan et al., 2021; Zhu, Abdelraheem, Wheeler et al., 2021), although its responses to FOV4 infections were described as tolerant or moderately tolerant in literature (Diaz et al., 2021; Ulloa et al., 2022, 2023). Other resistant Upland cultivars and breeding lines were recently identified (e.g., Abdelraheem et al., 2020; Ulloa et al., 2020, 2023; Zhang et al., 2016; Zhang, Idowu et al., 2020). Recently, we reported (Zhu et al., 2022) a study to compare differences in the infection process involved with FOV4 penetration, colonization, and infection to the cotton roots and expansion to other parts of cotton plants between two Pima cottons differing in resistance to FOV4.…”

Section: Introductionmentioning

confidence: 99%

“…In the two cultivated tetraploid cotton species, Pima S‐6 and its derived cultivars such as Pima PHY 800, PHY 76, and transgenic herbicide tolerant Pima cultivars are currently the only known source of resistance for FOV4 within Gossypium barbadense L. (Abdelraheem et al., 2021; Ulloa et al., 2006, 2013; Zhang, Abdelraheem et al., 2022; Zhang, Abdelraheem, Zhu, Wheeler, Dever, Elkins‐Arce et al., 2020; Zhang, Abdelraheem, Zhu, Wheeler, Dever, Nichols et al., 2021; Zhang, Zhu, Abdelraheem et al., 2022; Zhang, Zhu, Elkins‐Arce, Wheeler, Dever, Whitelock, Hake et al., 2022; Zhu et al., 2022, 2023; Zhu, Abdelraheem, Lujan et al., 2021; Zhu, Abdelraheem, Wheeler et al, 2021). FM 2334GLT is a commercial transgenic cultivar with FOV4 resistance within Upland cotton ( G. hirsutum L.) in the United States because it consistently showed reduced disease severity caused by FOV4 relative to susceptible plants (Diaz et al., 2021; Ulloa et al., 2022; Zhang, Abdelraheem, Zhu, Wheeler, Dever, Nichols et al., 2021; Zhang, Zhu, Abdelraheem et al., 2022; Zhu, Abdelraheem, Lujan et al., 2021; Zhu, Abdelraheem, Wheeler et al., 2021), although its responses to FOV4 infections were described as tolerant or moderately tolerant in literature (Diaz et al., 2021; Ulloa et al., 2022, 2023). Other resistant Upland cultivars and breeding lines were recently identified (e.g., Abdelraheem et al., 2020; Ulloa et al., 2020, 2023; Zhang et al., 2016; Zhang, Idowu et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of infection process in Upland cottons differing in resistance to Fusarium wilt caused byFusarium oxysporumf. sp.vasinfectumrace 4

Abdelraheem

Cooke

Wheeler³

et al. 2023

Crop Science

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Fusarium wilt (Fusarium oxysporum f. sp. vasinfectum race 4, FOV4) in cotton is an early season soil-borne vascular disease including seedling wilt and death. Its infection process in Upland cotton is currently unknown. This study compared the infection process among three Upland cotton cultivars (Gossypium hirsutum) using a confocal microscope. Seedlings were grown in a hydroponic system to the two-true leaf stage and inoculated with a virulent local FOV4 isolate. Based on the infection process, the resistant cultivar FM 2334GLT was divided into resistant (R) and susceptible (S) groups. The susceptible PHY 725 RF and FM 2334GLT(S) had the highest numbers of conidia attached and germinated on the root surface, followed by susceptible Acala 1517-08. FM 2334GLT(R) had the lowest numbers. Hyphae penetration and colonization inside the root followed a similar trend. In PHY 725 RF, FM 2334GLT(S), and Acala 1517-08, hyphae started to penetrate into epidermal cells at 2 days post inoculation (dpi), spread in the cortex intercellularly and intracellularly at 3-5 dpi, and entered the xylem vessels at 7 dpi, followed by colonization at 11-15 dpi. FM 2334GLT(R) had the lowest numbers of penetrating hyphae inside the root, with no apparent hyphae observed in the xylem. This study demonstrated that FM 2334GLT(R) had delayed infection, reduced fungal growth and reproduction, and prevented the FOV4 fungus from invading the xylem. The resistance in FM 2334GLT(R) was similar to that in Pima PHY 841 RF but with fewer hyphae in the cortex, suggesting possible different resistant mechanisms. INTRODUCTIONFusarium wilt (FW) of cotton (Gossypium spp.), caused by the soilborne fungus Fusarium oxysporum f. sp. vasinfectum (FOV), represents a serious fungal disease in cotton that limits

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“…Pima S-6 has been subjected to several cycles of evaluation and selection under naturally FOV4-infested fields to increase its uniformity and resistance level. This new source, renamed Pima-S6, has been incorporated into cotton breeding programs to combat the highly pathogenic FOV4 in the U.S. ( Ulloa et al, 2016b ; Ulloa et al, 2022a ; Ulloa et al, 2022b ).…”

Section: Introductionmentioning

confidence: 99%

Enhanced phenylpropanoid metabolism underlies resistance to Fusarium oxysporum f. sp. vasinfectum race 4 infection in the cotton cultivar Pima-S6 (Gossypium barbadense L.)

Ojeda-Rivera,

Ulloa,

Pérez-Zavala

et al. 2024

Front. Genet.

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Introduction:Fusarium oxysporum f. sp. vasinfectum (FOV) race 4 (FOV4) is a highly pathogenic soil-borne fungus responsible for Fusarium wilt in cotton (Gossypium spp.) and represents a continuing threat to cotton production in the southwest states of the United States, including California, New Mexico, and Texas. Pima (G. barbadense L.) cotton, which is highly valued for its fiber quality, has been shown to be more susceptible to this pathogen than Upland (G. hirsutum L.) cotton. Still, some Pima cultivars present resistance to FOV4 infection.Methods: To gain insights into the FOV4-resistance mechanism, we performed comparative transcriptional and metabolomic analyses between FOV4-susceptible and FOV4-resistant Pima cotton entries. FOV4-resistant Pima-S6 and FOV4-susceptible Pima S-7 and Pima 3-79 cotton plants were infected with FOV4 in the greenhouse, and the roots harvested 11 days post-infection for further analysis.Results: We found that an enhanced root phenylpropanoid metabolism in the resistant Pima-S6 cultivar determines FOV4-resistance. Gene-ontology enrichment of phenylpropanoid biosynthesis and metabolism categories correlated with the accumulation of secondary metabolites in Pima-S6 roots. Specifically, we found esculetin, a coumarin, an inhibitor of Fusarium’s growth, accumulated in the roots of Pima-S6 even under non-infected conditions. Genes related to the phenylpropanoid biosynthesis and metabolism, including phenylalanine ammonia-lyase 2 (PAL2) and pleiotropic drug resistance 12 (PDR12) transporter, were found to be upregulated in Pima-S6 roots.Discussion: Our results highlight an essential role for the phenylpropanoid synthesis pathway in FOV4 resistance in Pima-S6 cotton. These genes represent attractive research prospects for FOV4-disease resistance and breeding approaches of other cotton cultivars of economic relevance.

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Registration of 17 upland cotton germplasm lines with improved resistance to Fusarium wilt race 4 and good fiber quality

Cited by 8 publications

References 34 publications

Enhanced phenylpropanoid metabolism underlies resistance to Fusarium oxysporum vasinfectum f. sp. race 4 infection in the cotton cultivar Pima-S6 (Gossypium barbadense L.)

Enhanced phenylpropanoid metabolism underlies resistance to Fusarium oxysporum vasinfectum f. sp. race 4 infection in the cotton cultivar Pima-S6 (Gossypium barbadense L.)

Comparative analysis of infection process in Upland cottons differing in resistance to Fusarium wilt caused byFusarium oxysporumf. sp.vasinfectumrace 4

Enhanced phenylpropanoid metabolism underlies resistance to Fusarium oxysporum f. sp. vasinfectum race 4 infection in the cotton cultivar Pima-S6 (Gossypium barbadense L.)

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