Registration of Annual Ryegrass Genetic Stock IL2

Abstract

Husnot (syn. Lolium multifl orum Lam.)] genetic stock that exhibits the ability to induce genome or chromosome loss when hybridized with Lolium arundinaceum (Schreb.) S.J. Darbyshire (syn. Festuca arundinacea Schreb.) (2n = 6x = 42). Traditional recurrent selection methods for population or synthetic development, especially in polyploid species, involve large numbers of individuals and generate considerable expense in land and time. The development of improved germplasm resources, facilitated through nontraditional or novel genetic approaches, such as haploid induction, has occasiona lly been used to complement t rad it iona l breed ing a nd selection methods (Gordilli and Geiger, 2008). One objective of our research is to identify novel genetic methods that will enhance traditional breeding methods and facilitate the development of improved Lolium spp. germplasm resources for forage production. Double-haploid breeding

Generation of paternal dihaploids in tall fescue

“…Festuca arundinacea Schreb.]) (Kindiger and Singh ; Kindiger ). Tall fescue (2 n = 6 x = 42) represents the predominant, introduced perennial cool‐season grass forage in the USA.…”

“…In a situation where maternal haploids are the end product, the inducer line is typically the pollen parent (Fehr 1984;R€ ober et al 2005). When paternal haploids represent the final product, the maternal or egg parent represents the inducer line, such as is seen with the indeterminate gametophyte system of maize (Kermicle 1969 (Kindiger and Singh 2011;Kindiger 2012). Tall fescue (2n = 6x = 42) represents the predominant, introduced perennial cool-season grass forage in the USA.…”

Sampling the Genetic Diversity of Tall Fescue Utilizing Gamete Selection

“…Genome loss, spontaneous doubling followed by parthenogenic development represent the important and essential contributions of the IL1 and IL2 lines. All recovered lines derived from this process will have all genes, alleles or quantitative loci conferring a trait fixed in the DH recovery (Kindiger and Singh, 2011;Kindiger 2011). Essentially, the process generates homozygous or DH lines from the IL x tall fescue F1 hybrids.…”

“…Lolium multiflorum Lam.) (2n=2x=14) genetic stocks, identified as IL1 and IL2 (Kindiger and Singh, 2011;Kindiger, 2011), are characterized by a genome loss phenomenon following hybridization with tall fescue (Lolium arundinaceum (Schreb.)) S.J.…”

Notification of the Release of Annual Ryegrass Genetic Stock IL1

“…GS‐9, PI 660129) is known to generate dihaploid individuals at a low frequency (less than 1%); however, the method of identifying dihaploid lines in bulk germination tests is efficient and effective. The release of IL1 and its sister line IL2 (Kindiger and Singh, 2011) should provide an alternative approach for dihaploid generation within the Festuca – Lolium complex of polyploid grasses and should facilitate breeding advances in such material.…”