Registration of Golden Promise/Otis barley recombinant inbred line mapping population

Abstract

A recombinant inbred line mapping population was developed from a cross between the heritage malting barley (Hordeum vulgare ssp. vulgare) cultivar ‘Golden Promise’ and ‘Otis’, a feed barley cultivar developed in the 1960s suitable for the high dry plains. The Golden Promise/Otis recombinant inbred line (RIL) population (MP‐3, NSL 545640 MAP) consists of 190 F5:7 individuals. The parental lines exhibited significant differences in several agronomic traits including height, heading date, yield/plant, and lodging. Transgressive segregation in the RILs was evidenced by the broad range of values observed for these agronomic traits. The RIL population was genotyped using the Illumina 50k iSelect single nucleotide polymorphism (SNP) arrays. Of the 10,810 polymorphic markers that were identified between the two parents, 4617 markers were used for generating a genetic map that spanned 4566 cM with an average distance of 1 cM between markers. The fact that Golden Promise is a malting cultivar developed in the United Kingdom, versus Otis, a feed barley bred in the United States for dryland cultivation, indicates that this population may be useful in identifying the genetic basis of many other traits.