Registration of PR0633-10 and PR0737-1 Red Mottled Dry Bean Germplasm Lines with Resistance to BGYMV, BCMV, BCMNV, and Common Bacterial Blight

Prophète, Emmanuel; Demosthenes, G.; Godoy‐Lutz, G.; Porch, Timothy G.; Beaver, James S.

doi:10.3198/jpr2013.08.0046crg

Cited by 8 publications

(8 citation statements)

References 17 publications

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“…New, more virus-resistant CB varieties bred in USA, University of Samorano (Honduras), and International Center for Tropical Agriculture (CIAT) in Colombia have been released in Nicaragua during the past decade [ 7 , 8 , 37 ]. Before registration as cultivars, the new CB varieties introduced to Nicaragua are tested for adaptability and other important characteristics in La Compañia, a research station situated in Carazo—the Pacific region of Nicaragua.…”

Section: Introductionmentioning

confidence: 99%

Pathogenic seedborne viruses are rare but Phaseolus vulgaris endornaviruses are common in bean varieties grown in Nicaragua and Tanzania

et al. 2017

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Common bean (Phaseolus vulgaris) is an annual grain legume that was domesticated in Mesoamerica (Central America) and the Andes. It is currently grown widely also on other continents including Africa. We surveyed seedborne viruses in new common bean varieties introduced to Nicaragua (Central America) and in landraces and improved varieties grown in Tanzania (eastern Africa). Bean seeds, harvested from Nicaragua and Tanzania, were grown in insect-controlled greenhouse or screenhouse, respectively, to obtain leaf material for virus testing. Equal amounts of total RNA from different samples were pooled (30–36 samples per pool), and small RNAs were deep-sequenced (Illumina). Assembly of the reads (21–24 nt) to contiguous sequences and searches for homologous viral sequences in databases revealed Phaseolus vulgaris endornavirus 1 (PvEV-1) and PvEV-2 in the bean varieties in Nicaragua and Tanzania. These viruses are not known to cause symptoms in common bean and are considered non-pathogenic. The small-RNA reads from each pool of samples were mapped to the previously characterized complete PvEV-1 and PvEV-2 sequences (genome lengths ca. 14 kb and 15 kb, respectively). Coverage of the viral genomes was 87.9–99.9%, depending on the pool. Coverage per nucleotide ranged from 5 to 471, confirming virus identification. PvEV-1 and PvEV-2 are known to occur in Phaseolus spp. in Central America, but there is little previous information about their occurrence in Nicaragua, and no information about occurrence in Africa. Aside from Cowpea mild mosaic virus detected in bean plants grown from been seeds harvested from one region in Tanzania, no other pathogenic seedborne viruses were detected. The low incidence of infections caused by pathogenic viruses transmitted via bean seeds may be attributable to new, virus-resistant CB varieties released by breeding programs in Nicaragua and Tanzania.

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Section: Introductionmentioning

confidence: 99%

Pathogenic seedborne viruses are rare but Phaseolus vulgaris endornaviruses are common in bean varieties grown in Nicaragua and Tanzania

et al. 2017

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“…Molecular markers are powerful tools to analyze the genetic control of complex traits such as diseases resistance, seed iron and drought tolerance [9] , [10] , [11] . A reliable genetic map was developed to analyze the inheritance of yield traits under drought and fully irrigated conditions over three years of experiments [9] .…”

Section: Introductionmentioning

confidence: 99%

De Novo Assembly of the Common Bean Transcriptome Using Short Reads for the Discovery of Drought-Responsive Genes

Wang

et al. 2014

PLoS ONE

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The common bean (Phaseolus vulgaris L.) is one of the most important food legumes, far ahead of other legumes. The average grain yield of the common bean worldwide is much lower than its potential yields, primarily due to drought in the field. However, the gene network that mediates plant responses to drought stress remains largely unknown in this species. The major goals of our study are to identify a large scale of genes involved in drought stress using RNA-seq. First, we assembled 270 million high-quality trimmed reads into a non-redundant set of 62,828 unigenes, representing approximately 49 Mb of unique transcriptome sequences. Of these unigenes, 26,501 (42.2%) common bean unigenes had significant similarity with unigenes/predicted proteins from other legumes or sequenced plants. All unigenes were functionally annotated within the GO, COG and KEGG pathways. The strategy for de novo assembly of transcriptome data generated here will be useful in other legume plant transcriptome studies. Second, we identified 10,482 SSRs and 4,099 SNPs in transcripts. The large number of genetic markers provides a resource for gene discovery and development of functional molecular markers. Finally, we found differential expression genes (DEGs) between terminal drought and optimal irrigation treatments and between the two different genotypes Long 22-0579 (drought tolerant) and Naihua (drought sensitive). DEGs were confirmed by quantitative real-time PCR assays, which indicated that these genes are functionally associated with the drought-stress response. These resources will be helpful for basic and applied research for genome analysis and crop drought resistance improvement in the common bean.

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“…Four non-yellow P. vulgaris controls are also part of the YBP, which include a white bean landrace collected from Burundi (PI527521) and a white bean variety from Ecuador (Blanco Fanesquero). The other two controls include the red mottled JB178, a high yielding disease resistance variety released by the Dominican Republic in 1998 [ 30 ] and PR0737-1, a high yielding virus resistant red mottled line released jointly in 2013 by the University of Puerto Rico, USDA-ARS and the Haiti National Program [ 31 ]. The non-yellow controls were selected based upon their unique fast or slow cooking properties, which were measured during previous investigations [ 21 , 22 , 32 ].…”

Section: Methodsmentioning

confidence: 99%

The Fast Cooking and Enhanced Iron Bioavailability Properties of the Manteca Yellow Bean (Phaseolus vulgaris L.)

et al. 2018

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The common dry bean (Phaseolus vulgaris L.) is a nutrient-dense pulse crop that is produced globally for direct human consumption and is an important source of protein and micronutrients for millions of people across Latin America, the Caribbean and Sub-Saharan Africa. Dry beans require large amounts of heat energy and time to cook, which can deter consumers worldwide from using beans. In regions where consumers rely on expensive fuelwood for food preparation, the yellow bean is often marketed as fast cooking. This study evaluated the cooking time and health benefits of five major market classes within the yellow bean seed type (Amarillo, Canary, Manteca, Mayocoba, Njano) over two field seasons. This study shows how the Manteca yellow bean possesses a fast cooking phenotype, which could serve as genetic resource for introducing fast cooking properties into a new generation of dry beans with cooking times <20 min when pre-soaked and <80 min unsoaked. Mineral analysis revealed fast cooking yellow beans have high iron retention (>80%) after boiling. An in vitro digestion/Caco-2 cell culture bioassay revealed a strong negative association between cooking time and iron bioavailability in yellow beans with r values = −0.76 when pre-soaked and −0.64 when unsoaked across the two field seasons. When either pre-soaked or left unsoaked, the highest iron bioavailability scores were measured in the fast cooking Manteca genotypes providing evidence that this yellow market class is worthy of germplasm enhancement through the added benefit of improved iron quality after cooking.

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Registration of PR0633-10 and PR0737-1 Red Mottled Dry Bean Germplasm Lines with Resistance to BGYMV, BCMV, BCMNV, and Common Bacterial Blight

Cited by 8 publications

References 17 publications

Pathogenic seedborne viruses are rare but Phaseolus vulgaris endornaviruses are common in bean varieties grown in Nicaragua and Tanzania

Pathogenic seedborne viruses are rare but Phaseolus vulgaris endornaviruses are common in bean varieties grown in Nicaragua and Tanzania

De Novo Assembly of the Common Bean Transcriptome Using Short Reads for the Discovery of Drought-Responsive Genes

The Fast Cooking and Enhanced Iron Bioavailability Properties of the Manteca Yellow Bean (Phaseolus vulgaris L.)

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