Registration of Sugarbeet Doubled Haploid Line KDH13 with Resistance to Beet Curly Top

Eujayl, Imad A.; Strausbaugh, C.A.; Lü, Changli

doi:10.3198/jpr2015.09.0055crgs

Cited by 12 publications

(8 citation statements)

References 21 publications

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“…The efficiency of doubling for the present study was 27.7% of treated haploid explants. In comparison with others' attempts at sugar beet doubled haploid production (Eujayl et al, 2016), the efficiency of the present method is higher. The ES of the treatments on gynogenesis rate is large, which is notable for recalcitrant plants.…”

Section: Haploid and Doubled Haploid Productioncontrasting

confidence: 56%

“…Differential gynogenic response rates were also reported by other research groups that investigated the effect of BAP on sugar beet gynogenic embryo induction (for a recent review, see Aflaki et al, 2017). While most of the studies on sugar beet gynogenesis resulted in low response rates (Eujayl et al, 2016;Aflaki et al, 2017), others produced high levels of gynogenesis response up to 45.5% (Pedersen and Keimer, 1996). To avoid the pitfall of relatively inefficient gynogenesis in sugar beet and to improve the efficiency of the technique, some research programs benefitted considerably from gynogenic embryo induction of highly responsive doubled haploid (Hansen et al, 2000) or male sterile donor plants (Svirshchevskaya and Doleze, 2000).…”

Section: Haploid and Doubled Haploid Productionmentioning

confidence: 99%

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The effects of proline on in vitro proliferation and propagation of doubled haploid sugar beet (Beta vulgaris)

2018

Turk J Bot

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Doubled haploid induction is one of the available methods normally used for sugar beet breeding. Gynogenic haploid explants of sugar beet induced with 1 or 2 mg L -1 6-benzylaminopurine were treated with 5 g L -1 colchicine, then subcultured on a solidified MS medium plus 0.2 mg L -1 kinetin. Colchicine doubled the chromosome number of 27.7% of the treated haploid explants. With the aim of increasing the number of doubled haploid explants, the effects of five levels of proline (0.0, 0.1, 0.2, 0.3, or 0.4 mM) on the explants' proliferation, propagation, and shoot length were compared. With a large effect size (ES), proline at 0.3 mM induced the highest amount of proliferation, while proline-free medium resulted in the lowest amount of it. The highest propagation rates were observed for the explants treated on media with 0.2 and 0.3 mM proline (very large ES). Proline at 0.3 mM induced the shortest shoots (medium ES). A very strong positive correlation between proliferation and propagation, a moderate negative correlation between proliferation and length, and a strong negative correlation between propagation and length were observed. For the first time our results show beneficial effects of proline on in vitro proliferation and propagation of sugar beet.

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Section: Haploid and Doubled Haploid Productioncontrasting

confidence: 56%

Section: Haploid and Doubled Haploid Productionmentioning

confidence: 99%

The effects of proline on in vitro proliferation and propagation of doubled haploid sugar beet (Beta vulgaris)

2018

Turk J Bot

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“…This paper represents a pipeline for the fast and efficient characterization of beet genomes using Pac-bio long-read sequencing to ultimately inform the genetic basis of important agronomic characters. The double-haploid KDH13 (PI 663862) was created from a single plant selected for BCTV resistance from the population C762-17 (PI 560130) (Eujayl et al 2016). This line was sequenced and assembled to capture the genetic variation associated with BCTV resistance.…”

Section: Key Messagementioning

confidence: 99%

A roadmap to Durable BCTV Resistance Using Long-Read Genome Assembly of Genetic Stock KDH13

Galewski

Eujayl

2021

Plant Mol Biol Rep

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Beet Curly Top (BCT) is a viral disease which negatively impacts crop productivity for sugar beet growers and the sugar beet industry in the western USA and dry regions worldwide. Current varieties exhibit little genetic resistance to the Beet Curly Top Virus (BCTV), suggesting there is a large potential for improvement. KDH13 (PI 663862) is a double-haploid line created from a population (C762-17/PI 560130) which segregates for resistance to BCTV and was identified as genetic stock for the improvement of sugar beet varieties. PacBio sequences were generated and assembled to better define the content and organization of variation within the KDH13 genome and to provide resources to identify specific variation underpinning durable genetic resistance. Using ab initio predicted proteins as anchors, the assembled KDH13 contigs were placed in a more contiguous order using the EL10.1 reference genome, which leveraged Bio-Nano optical maps and Hi-C proximity information for chromosome level scaffolding. In total, 4681 (75%) of the 6245 contigs were placed in the order and orientation of the EL10.1 genome. The anchored contigs represented 502,929,268 bp (87.7%), the KDH13 genome assembly. An F1 hybrid and parental lines KDH13 (resistant) and KDH19-17 (susceptible) were sequenced using Illumina technology in order to characterize the SNP, indel, and structural variation between parental lines and allow for a more detailed investigation into causal variation linked to important phenotypes. In total, 3,086,720 variants were detected, including 2,259,324 single-nucleotide polymorphisms, 191,448 insertions, 198,057 deletions, 268,090 complex substitutions, 90,004 multi allelic variants, and 79,797 structural variants. Of the total variation, 1,158,491 were informative in the F1 and were able to discriminate between the two parents. This information represents a high-density marker dataset distributed globally across the sugar beet genome and can be used to track genomic segments in populations where KDH13 is used as parental material to improve BCTV resistance.

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“…Jak twierdzą Eujayl i in., 2016 procent otrzymanych tą drogą roślin, które uzyskują normalny pokrój, jest wciąż bardzo niski. W celu poprawy efektywności metody analizowanych jest wiele czynników kształtujących odpowiedź morfogenetyczną eksplantatu, w tym warunki kultury i zastosowane fitohormonów (Eujayl i in., 2016, Pazuki i in., 2018a, Pazuki i in., 2018b, Tomaszewska-Sowa, 2012. Reakcja eksplantatów na ww.…”

Section: Roślinne Kultury In Vitrounclassified

Evaluation of genetic stability of sugar beet (Beta vulgaris L.) plants obtained from unfertilized ovules using RAPD markers

Tomaszewska-Sowa¹,

Olszewska²

2019

JCEA

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In order to improve methods of embryo regeneration applied so far in in vitro cultures, a two-phased, innovative method involving liquid and solid media was used. Regeneration of ovules with the application of the gynogenesis technique takes place mainly through indirect organogenesis, which involves the formation of callus and the possibility of somaclonal variation. In the research, the DNA was isolated from the leaves of 10 regenerants, previously rooted and planted into the field. 5 of them were characterized by a haploid number of chromosomes whereas the others were of diploid nature. As a control, DNA originating from the leaves of a haploid plant growing in in vitro cultures on growth regulator-free MS medium was used. Plants regenerated on media of various quantitative and qualitative composition in terms of phytohormonal characteristic. The results of the experiment showed that 9 out of 19 tested primers generate polymorphic bands while the remaining 10 amplify the same DNA fragments in all tested samples. The polymorphism effect was revealed in 4 plants with diploid genome and in 1 haploid plant. In total, 13 polymorphic bands were identified and 11 of them were detected in diploid DNA.

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Registration of Sugarbeet Doubled Haploid Line KDH13 with Resistance to Beet Curly Top

Cited by 12 publications

References 21 publications

The effects of proline on in vitro proliferation and propagation of doubled haploid sugar beet (Beta vulgaris)

The effects of proline on in vitro proliferation and propagation of doubled haploid sugar beet (Beta vulgaris)

A roadmap to Durable BCTV Resistance Using Long-Read Genome Assembly of Genetic Stock KDH13

Evaluation of genetic stability of sugar beet (Beta vulgaris L.) plants obtained from unfertilized ovules using RAPD markers

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