Registration of the Barley Transposon‐Tagged Population II: Sixty‐One Lines Each Characterized for Ds Insertion Sites

Abstract

The USDA-ARS has developed and released a transposontagging barley (Hordeum vulgare L.) population comprising 61 lines: TNPs Each line is homozygous for a single modiied Ds element encoding bar (Ds-bar) insertion at a diferent genomic location, except for TNP 120, which has two insertions. The ~3.6 kb Ds-bar element is a modiied maize (Zea mays L.) Dissociation (Ds) element containing a bar expression cassette. Two of lines (TNP 32B and TNP 48) derive from primary Ds (PDS) transpositions resulting from biolistic co-introduction of Dsbar and maize Ac transposase (AcT ) into 'Golden Promise'. The rest contain either secondary or tertiary transpositions generated by remobilizing Ds-bar via hybridization of AcTexpressing Golden Promise plants with, respectively, PDS lines containing primary transpositions or TNP lines containing secondary transpositions. Each line was characterized for the 5¢ and/or 3¢ sequences lanking the Ds-bar insertion. Insertions that interrupt native genes or regulatory elements likely will alter or abolish their function, and these lines are expected to be useful for the study of the biological roles of such genes or regulatory sequences. Most of these lines can also be used as parents for the creation of additional lines with novel transpositions of Ds-bar.

Understanding the Adaptive Mechanisms of Plants to Enhance Phosphorus Use Efficiency on Podzolic Soils in Boreal Agroecosystems

“…PSTOL1 proteins possess structural features of wall-associated kinase (WAK) proteins such as Ser/Thr kinase, transmembrane, and extracellular domains important for their functionality in the cell wall (He et al, 1999 ). Analysis of single-copy Ds transposon mutant repository of barley (Singh et al, 2006 , 2012 ; Bregitzer et al, 2019 ) revealed that WAK proteins are implicated in the root growth of barley (Kaur et al, 2013 ; Tripathi et al, 2021 ), and that they are potentially new gene candidates for P starvation tolerance.…”

Identification of Transposable Elements in Conifer and Their Potential Application in Breeding

“…Previous studies have suggested that Ac/Ds can insert into gene-rich regions and alter the expression of genes or the genome through the formation of unstable insertion alleles, stable derivatives, or excision alleles. [23][24][25] Until now, Ac/Ds transposons have been shown to be effective in approximately 20 plant species and have become the most widely used TEs for gene tagging and functional genomics studies in plants. For example, Carter et al 25 produced activation-tagged and knockout mutants in the processing of tomato cultivar M82 using the Ac/Ds system and agrobacterium-mediated method.…”