Registration of the wheat D‐genome nested association mapping (DNAM) population

Abstract

Modern wheat is lacking diversity in the D genome due to the genetic bottleneck from the hybridization between tetraploid Triticum turgidum L. and diploid Aegilops tauschii Coss. The D‐genome nested association mapping (DNAM) population (Reg. no. MP‐14, NSL 536301 MAP) was developed to expand D‐genome variation in hexaploid wheat (Triticum aestivum L.). The DNAM population is a wheat nested association mapping population developed with direct crosses between the hard‐white winter Kansas State University breeding line KS05HW14‐3 and Ae. tauschii accessions TA10187, TA1693, TA10171, TA1662, TA1617, TA1615, TA1642, and TA1718. In total, there are 1,164 BC₂F₄ recombinant inbred lines (RILs) in 19 families. The DNAM was originally created for introgression of novel stem rust resistance genes but has since been used to identify resistance to other fungal pathogens. A subset of 420 lines were selected for important agronomic traits, including height and threshability, and named the DNAM Core RILs. Research with the DNAM has potential to provide novel genes that can be introgressed into elite cultivars, as well as knowledge and understanding of the D genome in wheat.

Description of U6719‐004 wheat germplasm with YrAS2388R stripe rust resistance introgression from Aegilops tauschii

“…A single BC 1 F 1 plant was recovered and backcrossed to KS05HW14‐3 as the male parent and designated U6719 (KS05HW14‐3/3/KS05HW14‐3/TA1718//KS05HW14‐3). A total of 14 BC 2 F 1 plants belonging to the U6719 family were advanced by single‐seed‐descent to the BC 2 F 4 generation when the seed from a single plant was harvested and increased in subsequent generations to produce BC 2 F 4 –derived lines (Strauss et al., 2020). Based on stripe rust disease screening, of the 14 lines belonging to the U6719 family, two lines were fixed for resistance (U6719‐004 and U6719‐008), and one line was segregating for resistance (U6719‐020).…”

“…U6719‐004 is a member of the D‐genome nested association mapping (DNAM) population released by Strauss et al. (2020) and was yield tested in several locations throughout the United States.…”

“…A small population of BC 2 F 4 –derived wheat introgression lines, designated as U6719 ( n = 14), was developed from the direct hybridization of the stripe rust–resistant Ae. tauschii accession TA1718 and susceptible wheat line KS05HW14‐3 (Strauss et al., 2020). To determine if resistance from TA1718 was successfully transferred and expressed in a hexaploid wheat background, U6719 wheat introgression lines were screened for seedling Pst resistance (six replicates per line).…”

“…Based on stripe rust disease screening, of the 14 lines belonging to the U6719 family, two lines were fixed for resistance (U6719-004 and U6719-008), and one line was segregating for resistance (U6719-020). U6719-004 is a member of the D-genome nested association mapping (DNAM) population released by Strauss et al (2020) and was yield tested in several locations throughout the United States.…”

“…tauschii and acceptable agronomic performance and grain quality. U6719-004 is a member of the DNAM population released by Strauss et al (2020). U6719-004 is a BC 2 F-derived wheat introgression line developed by direct hybridization and backcrossing of the stripe rust-resistant Ae.…”