Registration of Two Soybean Germplasm Lines Containing Leaf‐Chewing Insect Resistance QTLs from PI 229358 and PI 227687 Introgressed into ‘Benning’

Abstract

Two soybean [Glycine max (L.) Merr.] germplasm lines, Benning-ME (Reg. No. GP-405, PI 679958) and Benning-MGHE (Reg. No. GP-406, PI 679959), were developed by the University of Georgia Agricultural Experiment Stations.Control of insect pests is crucial in soybean production; hostplant resistance reduces the need for insecticide applications, thus diminishing production costs and pesticide concerns. In soybean, resistance to a broad range of leaf-chewing insects is found in the Japanese accessions PI 229358 and PI 227687. In PI 229358, resistance is conferred by quantitative trait loci (QTLs) M, H, and G. In PI 227687, resistance is conferred by QTL-E. To enhance soybean resistance to leafchewing insects, QTLs of PI 229358 and PI 227687 were pyramided in Benning-ME and Benning-MGHE, which are near-isogenic lines of 'Benning' obtained through markerassisted backcrossing. Under ield conditions, Benning-ME and Benning-MGHE sustain 67 and 57% less defoliation than Benning, respectively. To determine the QTL introgressions in each line, high-density single-nucleotide polymorphism (SNP) genotypes were obtained using the SoySNP50K iSelect BeadChip (Illumina). To facilitate selection of lines carrying a speciic QTL pyramid, Kompetitive Allele Speciic Polymerase Chain Reaction markers were developed for high-throughput genotyping. These lines are valuable genetic resources for breeding of host-plant resistance to insects in soybean. The combination of QTL-M and QTL-E provides agriculturally relevant levels of resistance, and with only two loci, the use of this pyramid is feasible in a breeding program.

CRISPR/Cas9-Mediated Targeted Mutagenesis of GmUGT Enhanced Soybean Resistance Against Leaf-Chewing Insects Through Flavonoids Biosynthesis

“…Statistically significant differences are marked with asterisks (ns p > 0.05, 0.01< * p < 0.05, * * p < 0.01; Student's t-test; ns, non-significant). Ortega et al (2017) reported that GmUGT is a functional SNP marker that exhibits activity against H. zea, but information about the GmUGT gene and its role in imparting resistance to H. armigera remains limited. Here, we first characterized the GmUGT gene in soybean and observed that it could respond to defoliator attacks rather than mechanical damage (Figure 4B).…”

“…To date, several insect-resistance QTLs, specific for leaf-chewing insects, have been identified in the soybean germplasm ( Rector et al, 2000 ; Zhu et al, 2008 ; Wang et al, 2015 ; Ortega et al, 2016 ; Sabljic et al, 2020 ; Ghione et al, 2021 ; Lucini et al, 2021 ). Interestingly, Ortega et al (2017) reported that the Glyma.07g110300 marker (encoding a UDP-glycosyltransferase) in QTL-M was a functional single-nucleotide polymorphism (SNP) that could be used for the marker-assisted selection of insect-resistance QTLs and Glyma.07g110300 exhibits a loss of function in insect-resistant soybean germplasms. Native resistance traits have been reported against H. zea , but information about the GmUGT gene and its roles in resistance to H. armigera remains unclear.…”

Metabolomics Differences of Glycine max QTLs Resistant to Soybean Looper

“…Plants of Benning, Benning M, Benning E, and Benning EM [ 14 ] were grown until the V5 growth stage [ 46 ] at 27 °C in an insecticide-free greenhouse under 16 h:8 h light:dark to prevent premature flowering. Each plant was grown in a 0.95 L styrofoam cup with three drainage holes punched in the bottom and filled with Fafard 3B potting mix (Conrad Fafard, Agawam, MA, USA).…”

“…E is the same QTL initially identified by Terry et al [ 13 ]. To better study these QTLs and their interactions, they were bred into a set of near-isolines [ 14 ]. When tested under laboratory and field conditions, the near isoline containing both M and E shows agriculturally effective levels of resistance to a gamut of defoliating caterpillars [ 15 ].…”