Regression of Hepatic Lesions after Treatment of Schistosoma mansoni or Schistosoma japonicum Infection in Mice: a Comparative Study

Andrade, Zilton A.; Cox, Teresa M.; Cheever, Allen

doi:10.4269/ajtmh.1993.49.1

Cited by 11 publications

(11 citation statements)

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“…The liver responds to injury with regulated tissue regeneration every time there is a major loss of hepatic tissue (18). Treatment reduces the morbidity of S. mansoni-infected mice because periovular granulomas very rapidly regress in size after reduction of the amount of collagen (4,19).…”

Section: Discussionmentioning

confidence: 99%

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Effect of praziquantel administration on hepatic stereology of mice infected with Schistosoma mansoni and fed a low-protein diet

Barros

Costa-Silva

Biolchini

et al. 2009

Braz J Med Biol Res

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A study was undertaken to investigate the effect of administering praziquantel (PZQ), focusing on the liver stereological findings of malnourished mice infected with Schistosoma mansoni. Thirty female Swiss Webster mice (age: 21 days; weight: 8-14 g) were fed either a low-protein diet (8%) or standard chow (22% protein) for 15 days. Five mice in each group were infected with 50 cercariae each of the BH strain (Brazil). PZQ therapy (80 mg/kg body weight, per day) was started on the 50th day of infection and consisted of daily administration for 5 days. Volume density (hepatocytes, sinusoids and hepatic fibrosis) was determined by stereology using a light microscope. Body weight gain and total serum albumin levels were always lower in undernourished mice. Our stereological study demonstrated that treatment increased both volume density of hepatocytes in mice fed standard chow (47.56%, treated group and 12.06%, control) and low-protein chow (30.98%, treated group and 21.44%, control), and hepatic sinusoids [standard chow (12.52%, treated group and 9.06%, control), low-protein chow (14.42%, treated group and 8.46%, control)], while hepatic fibrosis was reduced [standard chow (39.92%, treated group and 78.88%, control) and low-protein chow (54.60%, treated group and 70.10%, control)]. On the other hand, mice fed low-protein chow decreased density volume of hepatocytes and hepatic fibrosis. In conclusion, our findings indicate that treatment with PZQ ameliorates hepatic schistosomiasis pathology even in mice fed a low-protein diet.

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Section: Discussionmentioning

confidence: 99%

“…Additionally, it decreases inflammation and progressive matrix degradation (4). In contrast, protein-deficient mice cannot effectively clear adult worms after PZQ compared to nourished mice (5).…”

mentioning

confidence: 99%

Effect of praziquantel administration on hepatic stereology of mice infected with Schistosoma mansoni and fed a low-protein diet

Barros

Costa-Silva

Biolchini

et al. 2009

Braz J Med Biol Res

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“…Collagen content of the liver was determined by measurement of liver hydroxyproline by a colorimetric assay as previously described [31,32].…”

Section: Biochemical and Histological Assessment Of Liver Injury And mentioning

confidence: 99%

Upregulation of hepatic angiotensin-converting enzyme 2 (ACE2) and angiotensin-(1–7) levels in experimental biliary fibrosis

Herath

Warner

Lubel

et al. 2007

Journal of Hepatology

147

193

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“…Whereas early in the granulomatous response hepatic collagen synthesis and collagenase activity occur simultaneously, with the progress of the inflammation collagenase activity decreases but collagen production and deposition continue, leading to pronounced fibrosis 7,8 . Several histological and biochemical studies indicated that curative chemotherapy of mice at the acute, 9–12 but not chronic, 13 stage of the infection also induced slow resorption of the deposited fibrous tissue. At the microscopic and ultrastructural level, extracellular breakdown of collagen fibrils was observed 14,15 .…”

Section: Introductionmentioning

confidence: 99%

Expression of matrix metalloproteinases and their inhibitors during the resorption of schistosome egg‐induced fibrosis in praziquantel‐treated mice

et al. 2004

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SUMMARYSchistosomiasis mansoni is a tropical helminthic disease characterized by parasite egginduced granulomatous in¯ammation and cumulative ®brosis. Because ®brosis is in¯uenced by the imbalance between degradative matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs), we analysed the resorption of ®brous tissue and MMP/TIMP expression in the livers of S. mansoni-infected and praziquantel-cured mice. Worm elimination signi®cantly enhanced survival rate, ameliorated the granulomatous pathology and reduced collagen I, III and IV gene expression at 6 and 12 months posttreatment. Compared to 6 months infected, untreated controls, liver ®brous tissue was resorbed by 71Á4% at 12 months after treatment. At 3 months post-treatment, expression of the MMP-2, -3, -8, -10, -13, -14 and -16 genes decreased compared with untreated controls. By 6 months, a highly signi®cant increase in MMP-10 gene expression was manifest. At 12 months, messages for all MMP genes decreased in relation to untreated controls. TIMP-1, -2 and -3 gene expression drastically decreased between 3 and 6 months. At 1 year, only TIMP-1 expression was signi®cantly diminished. Overall, pro®brogenic tumour necrosis factor (TNF)-a, transforming growth factor (TGF)-b and inducible nitric oxide synthase (iNOS) gene expression decreased. Antigen-stimulated splenocytes secreted signi®cantly higher levels of interleukin (IL)-4, IL-5, IL-10 and IL-13 cytokines between 3 and 12 months after treatment. Production of interferon (IFN)-g was higher than in untreated controls 3 and 6 months after treatment. In conclusion, praziquantel-treated mice showed a slow resorption of liver ®brous tissue. Resorption is attributed to the precipitous drop in TIMP-1 gene expression level, which shifted the balance in favour of MMP message expression and presumed enhanced collagenase activity.

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Regression of Hepatic Lesions after Treatment of Schistosoma mansoni or Schistosoma japonicum Infection in Mice: a Comparative Study

Cited by 11 publications

References 0 publications

Effect of praziquantel administration on hepatic stereology of mice infected with Schistosoma mansoni and fed a low-protein diet

Effect of praziquantel administration on hepatic stereology of mice infected with Schistosoma mansoni and fed a low-protein diet

Upregulation of hepatic angiotensin-converting enzyme 2 (ACE2) and angiotensin-(1–7) levels in experimental biliary fibrosis

Expression of matrix metalloproteinases and their inhibitors during the resorption of schistosome egg‐induced fibrosis in praziquantel‐treated mice

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