Regrowing the damaged or lost body parts

Shanmukhan, Anju Pallipurath; Mathew, Mabel Maria; Radhakrishnan, Dhanya; Aiyaz, Mohammed; Prasad, Kalika

doi:10.1016/j.pbi.2019.12.007

Cited by 11 publications

(10 citation statements)

References 61 publications

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“…Wound signaling is initiated immediately after damage, with cells in the vicinity of the wound displaying remarkable plasticity and then reprogrammed to meet urgent repair tasks (Xu, 2018 ; Shanmukhan et al, 2020 ). Initial changes reflect a rapid physical and chemical response to wounding; they involve alterations in plasma transmembrane potential and intracellular Ca 2+ concentration, increase in apoplastic glutamate, and H 2 O 2 generation (Choi et al, 2017 ; Toyota et al, 2018 ; Xu, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…Initial changes reflect a rapid physical and chemical response to wounding; they involve alterations in plasma transmembrane potential and intracellular Ca 2+ concentration, increase in apoplastic glutamate, and H 2 O 2 generation (Choi et al, 2017 ; Toyota et al, 2018 ; Xu, 2018 ). In comparison, it takes hours to initiate regeneration responses (Shanmukhan et al, 2020 ). The increased activation of various genes, including stem cell regulators and the concomitant hormonal surge, was previously reported as a side effect of wounding.…”

Section: Discussionmentioning

confidence: 99%

“…The increased activation of various genes, including stem cell regulators and the concomitant hormonal surge, was previously reported as a side effect of wounding. Indeed, pathways linking wound signaling, WIND1 expression, and the production of plant hormones to promote regeneration have only recently been investigated (Ikeda and Ohme-Takagi, 2014 ; Ikeuchi et al, 2019 , 2020 ; Shanmukhan et al, 2020 ; Ye et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction

et al. 2021

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Melocactus glaucescens is an endangered cactus highly valued for its ornamental properties. In vitro shoot production of this species provides a sustainable alternative to overharvesting from the wild; however, its propagation could be improved if the genetic regulation underlying its developmental processes were known. The present study generated de novo transcriptome data, describing in vitro shoot organogenesis induction in M. glaucescens. Total RNA was extracted from explants before (control) and after shoot organogenesis induction (treated). A total of 14,478 unigenes (average length, 520 bases) were obtained using Illumina HiSeq 3000 (Illumina Inc., San Diego, CA, USA) sequencing and transcriptome assembly. Filtering for differential expression yielded 2,058 unigenes. Pairwise comparison of treated vs. control genes revealed that 1,241 (60.3%) unigenes exhibited no significant change, 226 (11%) were downregulated, and 591 (28.7%) were upregulated. Based on database analysis, more transcription factor families and unigenes appeared to be upregulated in the treated samples than in controls. Expression of WOUND INDUCED DEDIFFERENTIATION 1 (WIND1) and CALMODULIN (CaM) genes, both of which were upregulated in treated samples, was further validated by real-time quantitative PCR (RT-qPCR). Differences in gene expression patterns between control and treated samples indicate substantial changes in the primary and secondary metabolism of M. glaucescens after the induction of shoot organogenesis. These results help to clarify the molecular genetics and functional genomic aspects underlying propagation in the Cactaceae family.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction

et al. 2021

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“…High eATP doses (>500 μM) reduce cell viability and can trigger programed cell death (Sun et al, 2012;Deng et al, 2015). While there is no direct evidence that eATP alone affects plant growth/regeneration after wounding, data suggest that a combination of several cues like DAMPS, PAMPS, ion/osmolyte concentrations, or mechanical stresses trigger a defense and regeneration response (Marhavý et al, 2019;Shanmukhan et al, 2020;Zhou et al, 2020). NAD + and NADP + , as di-nucleotides and similarly to ATP acting as a classical cofactor, can be released to the environment after wounding, through membrane leakage or active processes such as exocytosis in animal model species (Haag et al, 2007).…”

Section: Oligogalacturonidesmentioning

confidence: 99%

Breaking Bad News: Dynamic Molecular Mechanisms of Wound Response in Plants

et al. 2020

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Recognition and repair of damaged tissue are an integral part of life. The failure of cells and tissues to appropriately respond to damage can lead to severe dysfunction and disease. Therefore, it is essential that we understand the molecular pathways of wound recognition and response. In this review, we aim to provide a broad overview of the molecular mechanisms underlying the fate of damaged cells and damage recognition in plants. Damaged cells release the so-called damage associated molecular patterns to warn the surrounding tissue. Local signaling through calcium (Ca2+), reactive oxygen species (ROS), and hormones, such as jasmonic acid, activates defense gene expression and local reinforcement of cell walls to seal off the wound and prevent evaporation and pathogen colonization. Depending on the severity of damage, Ca2+, ROS, and electrical signals can also spread throughout the plant to elicit a systemic defense response. Special emphasis is placed on the spatiotemporal dimension in order to obtain a mechanistic understanding of wound signaling in plants.

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“…Wound signaling is initiated immediately after damage, with cells in the vicinity of the wound displaying remarkable plasticity and then reprogrammed to meet urgent repair tasks (Xu, 2018;Shanmukhan et al, 2020). Initial changes reflect a rapid physical and chemical response to wounding; they involve alterations in plasma transmembrane potential and intracellular Ca 2+ concentration, increase in apoplastic glutamate, and H 2 O 2 generation (Choi et al, 2017;Toyota et al, 2018;Xu, 2018).…”

Section: Discussionmentioning

confidence: 99%

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Regrowing the damaged or lost body parts

Cited by 11 publications

References 61 publications

Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction

Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction

Breaking Bad News: Dynamic Molecular Mechanisms of Wound Response in Plants

Data_Sheet_1.PDF

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