Regulation and role of an RNA-binding protein Msa2 in controlling the sexual differentiation of fission yeast

Oowatari, Yasuo; Jeong, Hee-Tae; Tanae, Katsuhiro; Nakagawa, Tsuyoshi; Kawamukai, Makoto

doi:10.1007/s00294-011-0335-5

Cited by 16 publications

(18 citation statements)

References 37 publications

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“…These data are consistent with the findings that the phosphorylation mimic Nrd1 DD also induced SG formation in unstressed cells (Figure 2B, GFP-Nrd1 DD , 0.7±0.2%, arrows), although the effect is a little more prominent in Nrd1 DD overproduction. This difference can be explained by the recent findings that Nrd1 is phosphorylated not only by Pmk1 but also by Spk1 MAPK under nitrogen starved conditions [25]. In addition, when cells were exposed to a very short period of heat shock (3 min), Pek1 DD -expressing cells displayed a greater number of SG appearance (1.0±0.5%) than that of the cells harboring the control vector alone (0.3±0.2%), suggesting that Pek1 DD expression stimulated the translocation of Nrd1 to SGs under heat shock (Figure 3B).…”

Section: Resultsmentioning

confidence: 97%

Role of the RNA-Binding Protein Nrd1 in Stress Granule Formation and Its Implication in the Stress Response in Fission Yeast

et al. 2012

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We have previously identified the RNA recognition motif (RRM)-type RNA-binding protein Nrd1 as an important regulator of the posttranscriptional expression of myosin in fission yeast. Pmk1 MAPK-dependent phosphorylation negatively regulates the RNA-binding activity of Nrd1. Here, we report the role of Nrd1 in stress-induced RNA granules. Nrd1 can localize to poly(A)-binding protein (Pabp)-positive RNA granules in response to various stress stimuli, including heat shock, arsenite treatment, and oxidative stress. Interestingly, compared with the unphosphorylatable Nrd1, Nrd1DD (phosphorylation-mimic version of Nrd1) translocates more quickly from the cytoplasm to the stress granules in response to various stimuli; this suggests that the phosphorylation of Nrd1 by MAPK enhances its localization to stress-induced cytoplasmic granules. Nrd1 binds to Cpc2 (fission yeast RACK) in a phosphorylation-dependent manner and deletion of Cpc2 affects the formation of Nrd1-positive granules upon arsenite treatment. Moreover, the depletion of Nrd1 leads to a delay in Pabp-positive RNA granule formation, and overexpression of Nrd1 results in an increased size and number of Pabp-positive granules. Interestingly, Nrd1 deletion induced resistance to sustained stresses and enhanced sensitivity to transient stresses. In conclusion, our results indicate that Nrd1 plays a role in stress-induced granule formation, which affects stress resistance in fission yeast.

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Section: Resultsmentioning

confidence: 97%

Role of the RNA-Binding Protein Nrd1 in Stress Granule Formation and Its Implication in the Stress Response in Fission Yeast

et al. 2012

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“…In S. pombe , other RNA binding proteins have been demonstrated to play important roles in sexual differentiation either positively like Mei2 [3], or negatively like Msa1 and Msa2/Nrd1 [16], [17], [18]. All of them perform different biochemical functions and are regulated by different signals.…”

Section: Discussionmentioning

confidence: 99%

“…The activity of Ste11 is regulated at several levels [1]. First, Ste11 protein is phosphorylated by several kinases including Pat1, Spk1 and Cdc2 [6], [7], [8], [9], and second, Ste11 mRNA abundance is regulated by Atf1, Rst2, Pcr1 and Prr1 transcription factors [10], [11], [12], [13], [14], [15] and Msa1 and Msa2/Nrd1 RNA binding proteins [16], [17], [18].…”

Section: Introductionmentioning

confidence: 99%

The RNA Binding Protein Csx1 Promotes Sexual Differentiation in Schizosaccharomyces pombe

2012

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Sexual differentiation is a highly regulated process in the fission yeast Schizosaccharomyces pombe and is triggered by nutrient depletion, mainly nitrogen source.One of the key regulatory proteins in fission yeast sexual differentiation is the transcription factor Ste11. Ste11 regulates the transcription of many genes required for the initial steps of conjugation and meiosis, and its deficiency leads to sterility. Ste11 activity is mainly regulated at two levels: phosphorylation and abundance of its mRNA.Csx1 is an RNA binding protein that we have previously described to bind and regulate the turnover rate of the mRNA encoding the transcription factor Atf1 in the presence of oxidative stress.We have observed that Csx1-deficient cells have defects in sexual differentiation and are partially sterile. We investigated how Csx1 is regulating this process in S. pombe. Csx1 associates with ste11+ mRNA and cells lacking Csx1 are sterile with a reduced amount of ste11+ mRNA. Overexpression of ste11+ mRNA completely rescues the mating deficiencies of csx1Δ cells.Here, we present a novel mechanism of ste11+ mRNA positive regulation through the activity of Csx1, an RNA binding protein that also have key functions in the response to oxidative stress in fission yeast. This finding opens interesting question about the possible coordination of sexual differentiation and oxidative stress response in eukaryotes and the role of RNA binding proteins in the adaptation to environmental signals.

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“…Vacuoles were stained with FM4-64, and stress granules were observed by co-localization with Pabp-RFP. 38) The results indicated that Pka1 co-localized with the vacuoles, but not with Pabp-RFP.…”

Section: Cellular Dynamics Of Pka1-gfp Under Glucose Starvationmentioning

confidence: 96%

Complex Formation, Phosphorylation, and Localization of Protein Kinase A ofSchizosaccharomyces pombeupon Glucose Starvation

Gupta

Paul

Oowatari

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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Nine sam mutants that undergo sexual differentiation without requiring starvation in Schizosaccharomyces pombe were previously isolated. In this study, we identified a nonsense mutation on the pka1 locus in the sam6 mutant. pka1 encodes a catalytic subunit of protein kinase A (PKA). Replacement and overexpression of pka1 suppressed the KCl sensitivity and hyper-mating phenotype of sam6, confirming that sam6 is an allele of pka1. To characterize further the regulation of Pka1, we tested the physical interaction between Pka1 and Cgs1 (a regulatory subunit of PKA). Pka1 and Cgs1 physically interacted under glucose-limited conditions but not under glucose-rich conditions. In addition, the formation of a Pka1-Cgs1 complex was detected under glucose-limited conditions by Blue Native PAGE. Furthermore, the Pka1 protein was found to be phosphorylated under glucose-starved conditions, and at the same time its localization shifted from the nucleus towards the cytoplasm (mainly the vacuoles), suggesting a strong relationship among phosphorylation, complex formation, and the cytoplasmic distribution of Pka1.

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Regulation and role of an RNA-binding protein Msa2 in controlling the sexual differentiation of fission yeast

Cited by 16 publications

References 37 publications

Role of the RNA-Binding Protein Nrd1 in Stress Granule Formation and Its Implication in the Stress Response in Fission Yeast

Role of the RNA-Binding Protein Nrd1 in Stress Granule Formation and Its Implication in the Stress Response in Fission Yeast

The RNA Binding Protein Csx1 Promotes Sexual Differentiation in Schizosaccharomyces pombe

Complex Formation, Phosphorylation, and Localization of Protein Kinase A ofSchizosaccharomyces pombeupon Glucose Starvation

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