Regulation by Gonadal Steroids of Estrogen and Progesterone Receptors Along the Reproductive Tract in Female Lambs

Meikle, A.; Garófalo, E.G.; Rodrı́guez-Piñón, M.; Tasende, C.; Sahlin, Lena

doi:10.1186/1751-0147-42-161

Cited by 15 publications

(3 citation statements)

References 34 publications

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“…which are important for fertility and reproductive outcomes. Differential expression pattern of genes of the oviduct were earlier analyzed by adopting molecular techniques like cDNA hybridization (Meikle et al, 2001), semi quantitative RT-PCR (Lok et al, 2002;Briton-Jones et al, 2004), real time PCR (Gabler et al, 2003;Kenngott et al, 2011) and microarray technology (Bauersachs et al, 2003;2004) in several species. However, to our knowledge, such studies on oviductal transcriptome profiling are scarce in bubaline species.…”

Section: Expression Profiling Of Candidate Embryotrophic Genes Of Buf...mentioning

confidence: 99%

Expression profiling of candidate embryotrophic genes of buffalo oviduct during different stages of oestrous cycle

Kalyanaraman

Satheshkumar²,

Raj

et al. 2022

Buffalo Bull.

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The aim of the present study was to identify the gene expression profile of three candidate genes namely heat shock protein 70, oviductal glycoprotein and osteopontin in buffalo oviduct during different stages of the oestrous cycle. Slaughter house derived buffalo oviducts were categorized as belonging to early, mid and late phase of the oestrous cycle based on the morphological appearance of CL and progesterone concentration. The relative abundance of each of the candidate gene transcript was quantified by real time RT-PCR. Transcripts of all the three candidate genes were found to be expressed in OEC throughout the oestrous cycle. The results of the present study demonstrate that gene expression in the buffalo oviduct is clearly regulated during the oestrous cycle, as these candidate genes play a crucial role in several reproductive processes. It was found that Hsp 70 gene was significantly upregulated in OEC belonging to the early and late phases when compared to mid phases of the cycle. The expression of OVGP mRNA was found to be maximum during the early phase and it remained low during the mid and late phases while expression of OPN mRNA was maximum during the early phase and declined sequentially during the mid and late phase of oestrus cycle.

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Section: Expression Profiling Of Candidate Embryotrophic Genes Of Buf...mentioning

confidence: 99%

Expression profiling of candidate embryotrophic genes of buffalo oviduct during different stages of oestrous cycle

Kalyanaraman

Satheshkumar²,

Raj

et al. 2022

Buffalo Bull.

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“…medial region; Forde et al 2009) or a pool of tissues from different regions (Mesquita et al 2014) was used for treatment comparisons. However, regional gradients of gene expression levels, previously reported in the reproductive tract of cattle (Bauersachs et al 2005), pigs (Buhi and Alvarez 2003) and sheep (Meikle et al 1997(Meikle et al , 2001, might be highly relevant for receptivity and pregnancy success. For example, early embryo transfer work determined that when two embryos were transferred bilaterally, the greatest chances of twinning were achieved when at least one embryo was transferred to the tip of the horn relative to the CL (Newcomb et al 1980).…”

Section: Introductionmentioning

confidence: 96%

Spatio-specific regulation of endocrine-responsive gene transcription by periovulatory endocrine profiles in the bovine reproductive tract

Araujo

Sponchiado

Pugliesi

et al. 2016

Reprod. Fertil. Dev.

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In cattle, pro-oestrous oestradiol and dioestrous progesterone concentrations modulate endometrial gene expression and fertility. The aim was to compare the effects of different periovulatory endocrine profiles on the expression of progesterone receptor (PGR), oestrogen receptor 2 (ESR2), oxytocin receptor (OXTR), member C4 of aldo-keto reductase family 1 (AKR1C4), lipoprotein lipase (LPL), solute carrier family 2, member 1 (SLC2A1) and serpin peptidase inhibitor, clade A member 14 (SERPINA14): (1) between uterine horns ipsi- and contralateral to the corpus luteum (CL), (2) between regions of the ipsilateral horn and (3) in the vagina. Endometrium and vagina tissue samples were collected from cows that ovulated a larger (large follicle-large CL, LF-LCL; n=6) or smaller follicle (small follicle-small CL, SF-SCL; n=6) 7 days after oestrus. Cows in the LF-LCL group had a greater abundance of transcripts encoding ESR2, AKR1C4, LPL, SLC2A1 and SERPINA14, but a reduced expression of PGR and OXTR in the endometrium versus the SF-SCL group (PPGR and OXTR was greater in the contralateral compared with the ipsilateral horn (PPGR, ESR2, LPL, SLC2A1 and SERPINA14 (P<0.05). Different periovulatory endocrine profiles, i.e. LF-LCL or SF-SCL, did not influence gene expression in the vagina and had no interaction with inter- or intra-uterine horn gene expression. In conclusion, inter- and intra-uterine horn variations in gene expression indicate that the expression of specific genes in the bovine reproductive tract is location dependent. However, spatial distribution of transcripts was not influenced by distinct periovulatory sex-steroid environments.

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“…This can be achieved if emphasis is placed, among others, on improving the reproductive parameters of the flocks; that is, if the number of lambs born per ewe at each lambing is increased through various reproductive techniques and adequate knowledge in physiology and endocrinology, since they are essential to improve the reproductive efficiency of the flock (Meikle et al, 2001;Cortez-Romero and Gallegos-Sánchez, 2014).…”

Section: Introductionmentioning

confidence: 99%

Suckling and Excess Fat in the Postpartum Reproductive Activity of Hair Sheep

Osorio-Marín

Torres-Hernández

Becerril-Pérez

et al. 2021

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Objective: To evaluate the effect of excess fat and suckling control on postpartum reproductive activity of hair ewes. Design/Methodology/Approach: Eighty-one (81) hair ewes with average weight of 58±8 kg were used, which on postpartum day 7 were assigned to one of four treatments under a completely randomized design with 2×2 factorial arrangement. Factor A is the type of suckling [continuous suckling (CS) and suckling control (SC)] and factor B, excess fat (with or without). In CS, ewes remained with their offspring 24 hours a day (n=16). In continuous suckling plus fat (CSF), ewes remained with the offspring all day plus the addition of 8% in dietary fat (n=24). In SC, it was 30 min of suckling only twice per day (n=14). In suckling control plus fat (SCF), controlled suckling lasted 30 min, twice a day plus 8% fat (n=25). Results: The response to the treatments was similar (p > 0.05) in onset of estrus, return rate, gestation, prolificacy and fertilization; for the lambing rate variable, there were differences (p ≤ 0.05), the SCF treatment was higher (68%) compared to CS (50%) and CSF (50%), but equal (p < 0.05) to SC (57.1%). Implications: In this experiment, it was observed that the SCF treatment presented a better response, implying that controlling suckling and providing excess fat helps to restore ovarian activity sooner after lambing. Conclusions: Suckling control plus inclusion of dietary excess fat in ovulation induction protocols during early postpartum (around day 25 postpartum) can be included in routine reproductive management of flocks, improving the reproductive efficiency of hair sheep.

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Regulation by Gonadal Steroids of Estrogen and Progesterone Receptors Along the Reproductive Tract in Female Lambs

Cited by 15 publications

References 34 publications

Expression profiling of candidate embryotrophic genes of buffalo oviduct during different stages of oestrous cycle

Expression profiling of candidate embryotrophic genes of buffalo oviduct during different stages of oestrous cycle

Spatio-specific regulation of endocrine-responsive gene transcription by periovulatory endocrine profiles in the bovine reproductive tract

Suckling and Excess Fat in the Postpartum Reproductive Activity of Hair Sheep

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