Regulation by Neurotransmitter Receptors of Serotonergic or Catecholaminergic Neuronal Cell Differentiation

Mouillet-Richard, Sophie; Mutel, Vincent; Loric, Sylvain; Tournois, Claire; Launay, Jean‐Marie; Kellermann, Odile

doi:10.1074/jbc.275.13.9186

Cited by 89 publications

(141 citation statements)

References 32 publications

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“…Actually, predicting epitopes on the sole basis of their binding motif has a success rate of approximately 30% to reveal naturally processed peptides (Yewdell and Bennink, 1999). In this work, only 168 and 192 peptides were naturally processed and presented to the immune system by 1C11 cells, a neuronal cell line (Buc-Caron et al, 1990;Mouillet-Richard et al, 2000). Among the five tested NP-peptides, 168NP, 192NP and 217NP but not 103NP and 166NP induced T cells that were lytic in vivo for NP-but not native peptide-loaded target cells.…”

Section: Discussionmentioning

confidence: 95%

“…These results indicate that the immunizing peptides must be modified to ensure CTL recruitment. To determine if these peptides are naturally processed by cells expressing PrP, we used the mPrP-expressing 1C11 neuronal cell line (Buc-Caron et al, 1990;Mouillet-Richard et al, 2000), which express H-2D b class I but not I-A b class II molecules at the cell surface (data not shown). Wt mice were immunized with 168NP, 192NP, and 217NP mixed in HBV/CpG/IFA as described above.…”

Section: Generation Of Anti-prp Cytotoxic T Lymphocytes (Ctls)mentioning

confidence: 99%

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Prolongation of prion disease-associated symptomatic phase relates to CD3+ T cell recruitment into the CNS in murine scrapie-infected mice

Sacquin

Chaigneau

Defaweux

et al. 2012

Brain, Behavior, and Immunity

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a b s t r a c tPrion diseases are caused by the transconformation of the host cellular prion protein PrP c into an infectious neurotoxic isoform called PrP Sc . While vaccine-induced PrP-specific CD4 + T cells and antibodies partially protect scrapie-infected mice from disease, the potential autoreactivity of CD8 + cytotoxic T lymphocytes (CTLs) received little attention. Beneficial or pathogenic influence of PrP c -specific CTL was evaluated by stimulating a CD8 + T-cell-only response against PrP in scrapie-infected C57BL/6 mice. To circumvent immune tolerance to PrP, five PrP-derived nonamer peptides identified using prediction algorithms were anchored-optimized to improve binding affinity for H-2D b and immunogenicity (NP-peptides). All of the NP-peptides elicited a significant number of IFNc secreting CD8 + T cells that better recognized the NP-peptides than the natives; three of them induced T cells that were lytic in vivo for NP-peptide-loaded target cells. Peptides 168 and 192 were naturally processed and presented by the 1C11 neuronal cell line. Minigenes encoding immunogenic NP-peptides inserted into adenovirus (rAds) vectors enhanced the specific CD8 + T-cell responses. Immunization with rAd encoding 168NP before scrapie inoculation significantly prolonged the survival of infected mice. This effect was attributable to a significant lengthening of the symptomatic phase and was associated with enhanced CD3 + T cell recruitment to the CNS. However, immunization with Ad168NP in scrapie-incubating mice induced IFNc-secreting CD8 + T cells that were not cytolytic in vivo and did not influence disease progression nor infiltrated the brain. In conclusion, the data suggest that vaccine-induced PrP-specific CD8 + T cells interact with prions into the CNS during the clinical phase of the disease.

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Section: Discussionmentioning

confidence: 95%

Section: Generation Of Anti-prp Cytotoxic T Lymphocytes (Ctls)mentioning

confidence: 99%

Prolongation of prion disease-associated symptomatic phase relates to CD3+ T cell recruitment into the CNS in murine scrapie-infected mice

Sacquin

Chaigneau

Defaweux

et al. 2012

Brain, Behavior, and Immunity

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“…Comment parvenir à identifier celui qui cible les ARNm du SERT et comment déterminer si les SSRI exercent leur action en interférant avec l'expression d'un miR ? La lignée 1C11 de cellules souches neuronales : un outil précieux pour identifier le rôle de miR-16 sur l'expression du SERT Une lignée de cellules souches neuroectodermiques a été établie par notre équipe à partir de cellules embryonnaires pluripotentes murines [7]. Cette lignée, nommée 1C11, a la capacité, selon la nature des inducteurs, d'acquérir toutes les propriétés des neurones sérotoninergi-ques (1C11 5-HT ) en quatre jours, ou des neurones noradrénergiques (1C11 NE ) en douze jours.…”

Section: D'intérêtsunclassified

“…Une fonction conservée chez la drosophile Au cours de la neurogenèse chez la mouche Drosophila melanogaster, les divisions des neuroblastes, précur-seurs du système nerveux central, sont asymétriques [7,8]. Le neuroblaste se divise pour donner deux cellules de tailles et de compositions différentes : une cellule ganglionnaire et un neuroblaste.…”

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Le microARN-16

et al. 2011

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“…Data on the role of 5-HT in the differentiation and growth of dopamine (DA) neurons are contradictory (e.g., both stimulatory and inhibitory effects have been reported; Lauder et al, 1985;Liu and Lauder, 1992a,b;Witaker-Azmitia, 2001). Furthermore, it has been suggested that serotonergic and dopaminergic cells share a common precursor (Hynes et al, 1995;Mouillet-Richard et al, 2000). However, inductive signals that mediate the differentiation of neural precursors into 5-HT or DA neurons have not been clarified.…”

Section: Introductionmentioning

confidence: 99%

Serotonin decreases generation of dopaminergic neurons from mesencephalic precursors via serotonin type 7 and type 4 receptors

Parga

Rodríguez‐Pallares

Muñoz

et al. 2006

Developmental Neurobiology

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Inductive signals mediating the differentiation of neural precursors into serotonergic (5-HT) or dopaminergic neurons have not been clarified. We have recently shown that in cell aggregates obtained from rat mesencephalic precursors, reduction of serotonin levels induces a marked increase in generation of dopaminergic neurons. In the present study we treated rat neurospheres with antagonists of the main subtypes of 5-HT receptors, 5-HT transport inhibitors, or 5-HT receptor agonists, and studied the effects on generation of dopaminergic neurons. Cultures treated with Methiothepin (5-HT 1,2,5,6,7 receptor antagonist), the 5-HT 4 receptor antagonist GR113808, or the 5-HT 7 receptor antagonist SB 269970 showed a significant increase in generation of dopaminergic cells. Treatment with the 5-HT 1B/1D antagonist GR 127935, the 5-HT 2 antagonist Ritanserin, the 5-HT transporter inhibitor Fluoxetine, the dopamine and norepinephrine transport inhibitor GBR 12935, or with both inhibitors together, or 5-HT 4 or 5-HT 7 receptor agonists induced significant decreases in generation of dopaminergic cells. Cultures treated with WAY100635 (5-HT 1A receptor antagonist), the 5-HT 3 receptor antagonist Ondasetron, or the 5-HT 6 receptor antagonist SB 258585 did not show any significant changes. Therefore, 5-HT 4 and 5-HT 7 receptors are involved in the observed serotonininduced decrease in generation of dopaminergic neurons from proliferating neurospheres of mesencephalic precursors. 5-HT 4 and 5-HT 7 receptors were found in astrocytes and serotonergic cells using double immunolabeling and laser confocal microscopy, and the glial receptors appeared to play a major role. '

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Regulation by Neurotransmitter Receptors of Serotonergic or Catecholaminergic Neuronal Cell Differentiation

Cited by 89 publications

References 32 publications

Prolongation of prion disease-associated symptomatic phase relates to CD3+ T cell recruitment into the CNS in murine scrapie-infected mice

Prolongation of prion disease-associated symptomatic phase relates to CD3+ T cell recruitment into the CNS in murine scrapie-infected mice

Le microARN-16

Serotonin decreases generation of dopaminergic neurons from mesencephalic precursors via serotonin type 7 and type 4 receptors

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