Regulation mechanism of ABCA1 expression by statins in hepatocytes

Kobayashi, Masaki; Gouda, Keisuke; Chisaki, Ikumi; Ochiai, M.; Itagaki, Shirou; Iseki, Ken

doi:10.1016/j.ejphar.2011.04.043

Cited by 30 publications

(16 citation statements)

References 21 publications

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“…We then examined the effect of a PPAR-α antagonist (GW6471) 9) on the levels of OLHA-induced PPAR-α downstream gene mRNA levels in Clone 9 cells. As shown in Figs.…”

Section: Resultsmentioning

confidence: 99%

“…For the experiments, the Clone 9 cells (10 5 cells/10 cm dish) were cul-tured for 72 h, then the sub-confluent cells were incubated in serum-free medium at 37°C for 24 h before the addition of the test compounds. For treatment with GW6471, a PPAR-α antagonist, 9) 10 µM GW6471 was added with the tested compounds. After 48 h, the treated cells were harvested, counted, and used for total RNA extraction.…”

Section: )mentioning

confidence: 99%

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Synthesis and Evaluation of Fatty Acid Amides on the <i>N</i>-Oleoylethanolamide-Like Activation of Peroxisome Proliferator Activated Receptor α

Takao

Noguchi

Hashimoto

et al. 2015

CHEMICAL & PHARMACEUTICAL BULLETIN

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A series of fatty acid amides were synthesized and their peroxisome proliferator-activated receptor α (PPAR-α) agonistic activities were evaluated in a normal rat liver cell line, clone 9. The mRNAs of the PPAR-α downstream genes, carnitine-palmitoyltransferase-1 and mitochondrial 3-hydroxy-3-methylglutarylCoA synthase, were determined by real-time reverse transcription-polymerase chain reaction (RT-PCR) as PPAR-α agonistic activities. We prepared nine oleic acid amides. Their PPAR-α agonistic activities were, in decreasing order, N-oleoylhistamine (OLHA), N-oleoylglycine, Oleamide, N-oleoyltyramine, N-oleoylsertonin, and Olvanil. The highest activity was found with OLHA. We prepared and evaluated nine N-acylhistamines (N-acyl-HAs). Of these, OLHA, C16:0-HA, and C18:1Δ 9 -trans-HA showed similar activity. Activity due to the different chain length of the saturated fatty acid peaked at C16:0-HA. The PPAR-α antagonist, GW6471, inhibited the induction of the PPAR-α downstream genes by OLHA and N-oleoylethanolamide (OEA). These data suggest that N-acyl-HAs could be considered new PPAR-α agonists.

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“…We then examined the effect of a PPAR-α antagonist (GW6471) 9) on the levels of OLHA-induced PPAR-α downstream gene mRNA levels in Clone 9 cells. As shown in Figs.…”

Section: Resultsmentioning

confidence: 99%

Section: )mentioning

confidence: 99%

Synthesis and Evaluation of Fatty Acid Amides on the <i>N</i>-Oleoylethanolamide-Like Activation of Peroxisome Proliferator Activated Receptor α

Takao

Noguchi

Hashimoto

et al. 2015

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…Primers for FAT were forward: 5 0 -CTCTGACATTTGCAGGTCCA-3 0 and reverse: 5 0 -CACAGGCTTTCCTTCTTTGC-3 0 , whose amplification product is a 214-bp fragment (designed using Primer 3 Software, http://bioinfo.ut.ee/primer3-0.4.0/primer3). Primers for ABCA1 were forward: 5 0 -CAGGCTGATGT-CAGTCTCCA-3 0 and reverse: 5 0 -GGCTTCAGGATGTCCAT-GTT-3 0 , whose amplification product is a 194-bp fragment (Kobayashi et al 2011). The primers for the ribosomal protein L30, used as an internal control were forward: 5 0 -CCATCTTGGCGTCTGATCTT-3 0 and reverse: 5 0 -GGCG-AGGATAACCAATTTC-3 0 , whose amplification product is a 201-bp fragment, (Primer 3 Software).…”

Section: Expression Of Enzymes and Transporters Involved In Lipid Metmentioning

confidence: 99%

Peroxisome proliferator-activated receptor ligands regulate lipid content, metabolism, and composition in fetal lungs of diabetic rats

Kurtz¹,

Capobianco²,

Careaga³

et al. 2014

Journal of Endocrinology

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Maternal diabetes impairs fetal lung development. Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors relevant in lipid homeostasis and lung development. This study aims to evaluate the effect of in vivo activation of PPARs on lipid homeostasis in fetal lungs of diabetic rats. To this end, we studied lipid concentrations, expression of lipid metabolizing enzymes and fatty acid composition in fetal lungs of control and diabetic rats i) after injections of the fetuses with Leukotriene B 4 (LTB 4 , PPARa ligand) or 15deoxyD 12,14 prostaglandin J 2 (15dPGJ 2 , PPARg ligand) and ii) fed during pregnancy with 6% olive oil-or 6% safflower oil-supplemented diets, enriched with PPAR ligands were studied. Maternal diabetes increased triglyceride concentrations and decreased expression of lipid-oxidizing enzymes in fetal lungs of diabetic rats, an expression further decreased by LTB 4 and partially restored by 15dPGJ 2 in lungs of male fetuses in the diabetic group. In lungs of female fetuses in the diabetic group, maternal diets enriched with olive oil increased triglyceride concentrations and fatty acid synthase expression, while those enriched with safflower oil increased triglyceride concentrations and fatty acid transporter expression. Both olive oil-and safflower oil-supplemented diets decreased cholesterol and cholesteryl ester concentrations and increased the expression of the reverse cholesterol transporter ATP-binding cassette A1 in fetal lungs of female fetuses of diabetic rats. In fetal lungs of control and diabetic rats, the proportion of polyunsaturated fatty acids increased with the maternal diets enriched with olive and safflower oils. Our results revealed important changes in lipid metabolism in fetal lungs of diabetic rats, and in the ability of PPAR ligands to modulate the composition of lipid species relevant in the lung during the perinatal period.

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“…34, 35 Accordingly, pharmacological inhibition of ABCA1 degradation increases HDL biogenesis, leading to a therapeutic benefit by reducing cardiovascular diseases. 36, 37 Our present study revealed that tBHQ increases ABCA1 protein levels posttranslationally and enhances cholesterol efflux. However, Lee et al demonstrated that the antioxidant quercetin induced an increase in ABCA1 transcript level, which differs from tBHQ in the regulating mechanism.…”

Section: Increased Abca1 Protein Expression and Cholesterol Effluxmentioning

confidence: 85%

Tertiary-Butylhydroquinone Upregulates Expression of ATP-Binding Cassette Transporter A1 via Nuclear Factor E2-Related Factor 2/Heme Oxygenase-1 Signaling in THP-1 Macrophage-Derived Foam Cells

Lü¹,

Tang

Liu³

et al. 2013

Circ J

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Regulation mechanism of ABCA1 expression by statins in hepatocytes

Cited by 30 publications

References 21 publications

Synthesis and Evaluation of Fatty Acid Amides on the <i>N</i>-Oleoylethanolamide-Like Activation of Peroxisome Proliferator Activated Receptor α

Synthesis and Evaluation of Fatty Acid Amides on the <i>N</i>-Oleoylethanolamide-Like Activation of Peroxisome Proliferator Activated Receptor α

Peroxisome proliferator-activated receptor ligands regulate lipid content, metabolism, and composition in fetal lungs of diabetic rats

Tertiary-Butylhydroquinone Upregulates Expression of ATP-Binding Cassette Transporter A1 via Nuclear Factor E2-Related Factor 2/Heme Oxygenase-1 Signaling in THP-1 Macrophage-Derived Foam Cells

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