Regulation mechanism of silkworm pupa oil PUFAs on cholesterol metabolism in hepatic cell L‐02

Luo, Ying; Wang, Lifang; Lv, Yongzhong; Wu, Xiaoyun; Hou, Chen; Li, Jianke

doi:10.1002/jsfa.10115

Cited by 16 publications

(14 citation statements)

References 26 publications

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“…Zou et al (2017) described that silkworm pupae are sources of polyunsaturated fatty acids at 60-70% of total fat contents. In this study, the most abundant fatty acid was oleic acid (C18:1) (n-9), followed by alpha-linolenic acid, ALA, (C18:3) (n-3) and linoleic acid (C18:2) (n-6), respectively (Tables 3 and 4), as also observed by Luo et al (2020). ALA is known as a precursor to both eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which have shown vital physiological roles in human brain development and cardiovascular health (Ji et al, 2016;Luo et al, 2020).…”

Section: Nutritional Valuessupporting

confidence: 81%

“…In this study, the most abundant fatty acid was oleic acid (C18:1) (n-9), followed by alpha-linolenic acid, ALA, (C18:3) (n-3) and linoleic acid (C18:2) (n-6), respectively (Tables 3 and 4), as also observed by Luo et al (2020). ALA is known as a precursor to both eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which have shown vital physiological roles in human brain development and cardiovascular health (Ji et al, 2016;Luo et al, 2020). Therefore, it seems that silkworm pupae may be considered as a quality source of protein and lipid for human and could be used in the food industry as a nutritious additive in foods and beverages.…”

Section: Nutritional Valuessupporting

confidence: 81%

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Selected nutritional quality and physicochemical properties of silkworm pupae (frozen or powdered) from two species

Hirunyophat

Chalermchaiwat

On-Nom

et al. 2021

Int J of Food Sci Tech

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This research was performed to examine selected nutritional quality and physicochemical properties of silkworm pupae. Frozen or powdered pupae from two species of silkworm (Bombyx mori and Samia ricinii) were studied. Eri had the largest size with the highest lightness and peak viscosity. The PCA (principal component analysis) total variance could be explained by 74.68%, and silkworm pupaes could be put into 2 clusters. Cluster 1: all varieties of frozen silkworm pupae having a high moisture content; Cluster 2: silkworm pupae powder. Thai and Hybrid silkworm pupae powder had the highest calcium, total fat and n-3, n-6 and n-9 fatty acids. Eri silkworm pupae powder had the highest protein, essential amino acid and crude fibre. Each variety of silkworm pupae had different properties that might be suitable for developing different food products. Therefore, the presented data would be advantageous for manufacturers for developing functional ingredients and novel foods.

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Section: Nutritional Valuessupporting

confidence: 81%

Section: Nutritional Valuessupporting

confidence: 81%

Selected nutritional quality and physicochemical properties of silkworm pupae (frozen or powdered) from two species

Hirunyophat

Chalermchaiwat

On-Nom

et al. 2021

Int J of Food Sci Tech

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“…CYP7A1, an important enzyme for the conversion of cholesterol into bile acids, is a biomarker for reverse cholesterol transport ( Chen and Huang, 2009 ). Several studies have reported that PPARγ regulates the expression of CYP7A1, which suggests that miR-27b/PPARγ/CYP7A1 might comprise a pathway that regulates the metabolism of both cholesterol and polyunsaturated fatty acids ( Ge et al, 2017 ; Han et al, 2019 ; Luo et al, 2019 ). Our data demonstrated that elevated PPARγ levels enhanced the mRNA and protein expression of CYP7A1, which may promote the reverse transport of cholesterol in skeletal muscle while lowering the accumulation of total cholesterol in the blood ( Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of miR-27b Regulates Lipid Metabolism in Skeletal Muscle of Obese Rats During Hypoxic Exercise by Increasing PPARγ Expression

Xuebing

Zhu

et al. 2020

Front. Physiol.

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Hypoxic exercise may represent a novel therapeutic strategy to reduce and prevent obesity through the regulation of lipid metabolism. During hypoxic exercise, the targeting of peroxisome proliferator-activated receptor gamma (PPARγ) by miR-27b has been proposed to be one of the mechanisms involved in the modulation of lipid metabolism. We have previously shown that miR-27b can repress PPARγ and lipid metabolism-associated factors, thereby affecting lipid metabolism during hypoxic exercise in a rat model of obesity. In the current study, we aimed to confirm the role of miR-27b in the regulation of lipid metabolism. First, miR-27b expression was either upregulated or downregulated through the injection of adeno-associated virus (AAV) 9 containing a miR-27b expression cassette or miR-27b-3p inhibitor, respectively, into the right gastrocnemius muscle of obese rats. The rats were then subjected to a 4-week program of hypoxic exercise, and a series of parameters related to lipid metabolism were systematically evaluated, including body composition, blood lipid levels, miR-27b RNA levels, and mRNA and protein levels of PPARγ and those of its downstream lipid metabolism-associated factors. No significant differences were found in body composition between rats expressing different levels of miR-27b. However, regarding blood lipids, miR-27b overexpression led to increased concentrations of triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), and free fatty acids (FFAs), while inhibition of miR-27b decreased the total cholesterol (TC) level and increased that of high-density lipoprotein cholesterol (HDL-C). At the mRNA level, miR-27b overexpression downregulated the expression of Ppar γ, but upregulated that of lipid metabolism-associated factors such as heart-type fatty acid-binding protein (H-FABP), fatty acid transport protein 1 (FATP1), adipose triglyceride lipase (ATGL), and lipoprotein lipase (LPL), whereas miR-27b inhibition elicited the opposite effect; however, inhibition of miR-27b led to elevated cholesterol 7 alpha-hydroxylase (CYP7A1) and fatty acid translocase 36 (CD36) levels. Similarly, at the protein level, miR-27b overexpression promoted a decrease in the concentration of PPARγ, whereas miR-27b inhibition led to an increase in PPARγ levels, as well as those of CYP7A1, CD36, ATGL, and LPL. Overall, our results indicated that hypoxic exercise regulates lipid metabolism via the miR-27b/PPARγ pathway and modulates ATGL and LPL expression through inducing their post-transcriptional modifications.

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“…The effect of edible insects on coagulation markers (37)(38)(39) , inflammatory status (41,46) and lipid metabolism (7,(47)(48)(49) are described in Table 5. A total of eleven studies involving eight insects were reported: Protaetia brevitaris was cited in three research articles, whilst Oxya chinensis, Gryllus bimaculatus and Tenebrio molitor were investigated in two studies each.…”

Section: Other Activity In Cellular Models or Ex Vivomentioning

confidence: 99%

“…In four studies (7,(47)(48)(49) , the ability of edible insects to modulate lipid pattern in cellular models was investigated through evaluation of lipid content or the expression of genes related to lipid metabolism. Ethanolic extract of Gryllus bimaculatus, Oxya chinensis sinuosa and Protaetia brevitarsis seulensis diminished intracellular lipid accumulation and triacylglycerol (TG) in HepG2, a human liver cancer cellular line (47) .…”

Section: Other Activity In Cellular Models or Ex Vivomentioning

confidence: 99%

Functional properties of edible insects: a systematic review

et al. 2021

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Consumption of edible insects has been widely suggested as an environmentally sustainable substitute for meat to reduce GHG emissions. However, the novel research field for edible insects rely on the content of bioactive ingredients and on the ability to induce a functional effect in humans. The goal of this manuscript was to review the available body of evidence on the properties of edible insects in modulating oxidative and inflammatory stress, platelet aggregation, lipid and glucose metabolism and weight control. A search for literature investigating the functional role of edible insects was carried out in the PUBMED database using specific keywords. A total of 55 studies, meeting inclusion criteria after screening, were divided on the basis of the experimental approach: in vitro studies, cellular models/ex vivo studies or in vivo studies. In the majority of the studies, insects demonstrated the ability to reduce oxidative stress, modulate antioxidant status, restore the impaired activity of antioxidant enzymes and reduce markers of oxidative damage. Edible insects displayed anti-inflammatory activity reducing cytokines and modulating specific transcription factors. Results from animal studies suggest that edible insects can modulate lipid and glucose metabolism. The limited number of studies focused on the assessment of anticoagulation activity of edible insects make it difficult to draw conclusions. More evidence from dietary intervention studies in humans is needed to support the promising evidence from in vitro and animal models about the functional role of edible insects consumption.

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Regulation mechanism of silkworm pupa oil PUFAs on cholesterol metabolism in hepatic cell L‐02

Cited by 16 publications

References 26 publications

Selected nutritional quality and physicochemical properties of silkworm pupae (frozen or powdered) from two species

Selected nutritional quality and physicochemical properties of silkworm pupae (frozen or powdered) from two species

Inhibition of miR-27b Regulates Lipid Metabolism in Skeletal Muscle of Obese Rats During Hypoxic Exercise by Increasing PPARγ Expression

Functional properties of edible insects: a systematic review

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