Regulation of 6-phosphofructo-1-kinase in the placenta and small intestine of pregnant streptozotocin-induced diabetic rats

Khoja, Samir M.; Salem, Ahmed M.

doi:10.1016/0168-8227(91)90038-f

Cited by 7 publications

(3 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The elevation of plasma glucose levels in treated animals was significant (P < 0.0005) as compared to control. These results are similar to those reported by Khoja and Salem [14] .…”

Section: Plasma Glucose Levelssupporting

confidence: 83%

Activity Changes of Molybdenum Hydroxylases in Liver, Kidney and Jejunum of Streptozotocin - Induced Diabetic Rats

Al-Bar¹

2005

sci

View full text Add to dashboard Cite

ABSTRACT. The aim of this study is to investigate the effect of diabetes induction on the specific activity of two molybdenum hydroxylases, namely aldehyde oxidase and xanthine oxidase. Streptozotocin in a single i.p. injection (65 mg/kg) induced diabetes in male Wistar rats as indicated by a sharp and significant increase of plasma glucose level after 5 days of the treatment. Three tissues (liver, kidney and jejunum) were excised and assayed for the activity of aldehyde oxidase and xanthine oxidase. Two substrates (phthalazine and 3-methylisoquinoline) were used to measure aldehyde oxidase activity, while xanthine was used for xanthine oxidase. Hepatic aldehyde oxidase activity was increased significantly (P < 0.025) in diabetic rats as compared to controls when phthalazine was used as a substrate. The other two tissues (kidney, jejunum) did not show any significant variation in aldehyde oxidase activity when diabetic and control rats were compared. No significant change of this enzymic activity was noted in the three tissues (liver, kidney and jejunum) when 3-methylisoquinoline was used as a substrate. On the other hand, xanthine oxidase activity in liver and jejunum was markedly decreased (P < 0.005 and P < 0.0005, respectively) in diabetic rats while the enzymic activity of kidney remained unchanged. These results suggest the presence of multiple isomeric forms of molybdenum hydroxylases and some of these forms can be induced or suppressed by the diabetic inducing agent streptozotocin.

show abstract

“…The elevation of plasma glucose levels in treated animals was significant (P < 0.0005) as compared to control. These results are similar to those reported by Khoja and Salem [14] .…”

Section: Plasma Glucose Levelssupporting

confidence: 83%

Activity Changes of Molybdenum Hydroxylases in Liver, Kidney and Jejunum of Streptozotocin - Induced Diabetic Rats

Al-Bar¹

2005

sci

View full text Add to dashboard Cite

show abstract

“…Numerous studies implicate that diabetes reduces PFK-1 activity in different cell tissues (Sochor et al, 1987; Rossi et al, 1990; Khoja and Salem, 1991; Morimoto et al, 1995). PFK-1 can be considered a potential target for the treatment of obesity.…”

Section: Discussionmentioning

confidence: 99%

The Impact of DJOS Surgery, a High Fat Diet and a Control Diet on the Enzymes of Glucose Metabolism in the Liver and Muscles of Sprague-Dawley Rats

et al. 2019

View full text Add to dashboard Cite

The prevalence of diabetes type 2 (T2DM) and obesity is growing exponentially and becoming a global public health problem. The enzymes of glucose metabolism play a role in the pathogenesis of insulin resistance and T2DM. A pathophysiological link between different dietary patterns, HFD, obesity, T2DM and the enzymes of glucose metabolism can be used as a potential target in therapeutic strategies for the treatment of obesity, and T2DM. The aim of this study was to measure the impact of DJOS bariatric surgery and different types of dietary patterns on glycogen synthase kinase 3 α (GSK-3α), glycogen phosphorylase (PYGM, PYGL), and phosphofructokinase (PFK-1) concentrations in liver and soleus muscle tissues of rats. After 8 weeks on a high-fat diet (HF) or control diet (CD), rats underwent duodenal-jejunal omega switch (DJOS) or SHAM (control) surgery. After surgery, for the next 8 weeks, half of DJOS/SHAM animals were kept on the same diet as before, and half had a changed diet. The concentrations of GSK-3α, PYGM, PYGL and PFK-1 were measured in the soleus muscles and livers of the Sprague-Dawley rats. The type of diet applied before/after surgery had stronger impact on levels of selected metabolic enzymes than DJOS or SHAM surgery. The impact of DJOS surgery was visible for GSK-3α and PYGL concentration in the liver but not in the soleus muscle tissue. The type of bariatric surgery had an impact on liver GSK-3α concentration in all studied groups except the CD/CD group, where the impact of diet was stronger. DJOS bariatric surgery influenced the level of PYGL in the livers of rats maintained on the CD/CD diet but not from other groups. The dietary patterns applied before and after bariatric surgery, had a stronger impact on enzymes’ concentrations than DJOS surgery, and the strong, deleterious effect of an HF was observed. A change of the diet per se showed a negative impact on the enzymes’ tissue concentration.

show abstract

“…On the other hand, diabetes has a complex and variable effect on PFK-1 activity depending on the organ examined. Hence, reduced PFK-1 activity was reported in the enterocytes [21], adipocytes [22], liver [23], heart atria [24], intestinal mucosa and placenta [25].…”

Section: Diabetes Research and Clinical Metabolism Issn 2050-0866mentioning

confidence: 99%

Regulation of hepatic and mucosal 6-phosphofructo-1-kinase activity by trigonella foenum-graecum linn (fenugreek) seeds of streptozotocin-induced diabetic rats

Ali¹,

Zamzami²,

Khoja³

2013

J Diab Res Clin Met

View full text Add to dashboard Cite

Background: Trigonella foenum-graecum Linn. (fenugreek) can ameliorate hyperglycemia and dyslipidemia, however, the detailed mechanism is unclear. The present study aims to investigate the effect of fenugreek seeds water extract (FSE) on 6-phosphofructo-1-kinase (PFK-1) activity, a key enzyme of glycolysis, in the liver and small intestine of streptozotocin induced diabetic rats and its impact on plasma glucose and lipid concentrations. Methods: 50 male Wistar rats were used in this study. The rats were divided into five groups with 10 rats in each group: normal control rats (Group1), streptozotocin-induced diabetic rats (Group2), streptozotocin-induced diabetic rats + Fenugreek seeds (0.5g/500 ml water) (Group3), streptozotocin-induced diabetic rats + Fenugreek seeds (1.0g/500 ml water) (Group4), streptozotocin-induced diabetic rats + insulin (Group5). Blood samples were then collected after the period of treatment for the analysis of glucose, total cholesterol, triacylglycerol and insulin concentration. PFK-1 for liver and intestinal mucosa was extracted and assayed. Results: Diabetic rats showed significantly lower liver and intestinal mucosa PFK-1 activities (both at P<0.05) compared with control rats. Diabetic rats treated with FSE (1.0g / 500 ml water) showed a significant (P<0.0001) increase in the PFK-1 activity of liver and intestinal mucosa by 54% and 75% respectively. The plasma glucose concentrations of diabetic rats treated with 0.5g and 1.0g fenugreek / 500 ml water were significantly (P<0.001) decreased by 32% and 43%. In addition the concentrations of plasma total cholesterol and triacylglycerol were significantly (both at P<0.0001) decreased by 57% and 42%, respectively in rats treated with 1.0g fenugreek / 500 ml water, compared with non-treated diabetic rats. Conclusions: These results may suggest that the in-vivo hypoglycaemic effect of FSE is mediated, at least in part, by the activation of PFK-1 in intestinal and liver cells.

show abstract

Regulation of 6-phosphofructo-1-kinase in the placenta and small intestine of pregnant streptozotocin-induced diabetic rats

Cited by 7 publications

References 31 publications

Activity Changes of Molybdenum Hydroxylases in Liver, Kidney and Jejunum of Streptozotocin - Induced Diabetic Rats

Activity Changes of Molybdenum Hydroxylases in Liver, Kidney and Jejunum of Streptozotocin - Induced Diabetic Rats

The Impact of DJOS Surgery, a High Fat Diet and a Control Diet on the Enzymes of Glucose Metabolism in the Liver and Muscles of Sprague-Dawley Rats

Regulation of hepatic and mucosal 6-phosphofructo-1-kinase activity by trigonella foenum-graecum linn (fenugreek) seeds of streptozotocin-induced diabetic rats

Contact Info

Product

Resources

About