Regulation of a Trehalose-Specific Facilitated Transporter (TRET) by Insulin and Adipokinetic Hormone in Rhodnius prolixus, a Vector of Chagas Disease

Leyria, Jimena; El-Mawed, Hanine; Orchard, Ian; Lange, Angela B.

doi:10.3389/fphys.2021.624165

Cited by 24 publications

(15 citation statements)

References 94 publications

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“…It is well known that reproductive processes are energetically demanding, so it is therefore no surprise that they undergo complex pathways related to lipid and carbohydrate availability, reallocation, and metabolism ( Fullbright et al, 1997 ; Nässel and Vanden Broeck, 2016 ; Leyria et al, 2021a ). It was shown that ILPs and ILP signalling are necessary for correct functioning of both the female and male reproductive systems.…”

Section: Metabolic Processes Regulated By Ilpsmentioning

confidence: 99%

Insulin-Like Peptides and Cross-Talk With Other Factors in the Regulation of Insect Metabolism

et al. 2021

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The insulin-like peptide (ILP) and insulin-like growth factor (IGF) signalling pathways play a crucial role in the regulation of metabolism, growth and development, fecundity, stress resistance, and lifespan. ILPs are encoded by multigene families that are expressed in nervous and non-nervous organs, including the midgut, salivary glands, and fat body, in a tissue- and stage-specific manner. Thus, more multidirectional and more complex control of insect metabolism can occur. ILPs are not the only factors that regulate metabolism. ILPs interact in many cross-talk interactions of different factors, for example, hormones (peptide and nonpeptide), neurotransmitters and growth factors. These interactions are observed at different levels, and three interactions appear to be the most prominent/significant: (1) coinfluence of ILPs and other factors on the same target cells, (2) influence of ILPs on synthesis/secretion of other factors regulating metabolism, and (3) regulation of activity of cells producing/secreting ILPs by various factors. For example, brain insulin-producing cells co-express sulfakinins (SKs), which are cholecystokinin-like peptides, another key regulator of metabolism, and express receptors for tachykinin-related peptides, the next peptide hormones involved in the control of metabolism. It was also shown that ILPs in Drosophila melanogaster can directly and indirectly regulate AKH. This review presents an overview of the regulatory role of insulin-like peptides in insect metabolism and how these factors interact with other players involved in its regulation.

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Section: Metabolic Processes Regulated By Ilpsmentioning

confidence: 99%

Insulin-Like Peptides and Cross-Talk With Other Factors in the Regulation of Insect Metabolism

et al. 2021

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“…The ligand adipokinetic hormone (AKH) synthesized and released by the corpora cardiaca (CC) and AKH receptor (AKHR) as rhodopsin‐like GPCR has been proven to be involved in the regulation of energy metabolism, feeding, reproduction, diapause, flight and other activities. Particularly under stress conditions, AKH and AKHR can couple and participate in antioxidant stress responses by activating AC, PKA and cAMP 37–45 . Recent studies have shown that AKH and its receptor AKHR are involved in regulating resistance to insecticides 38,46–49 .…”

Section: Introductionmentioning

confidence: 99%

“…Particularly under stress conditions, AKH and AKHR can couple and participate in antioxidant stress responses by activating AC, PKA and cAMP. [37][38][39][40][41][42][43][44][45] Recent studies have shown that AKH and its receptor AKHR are involved in regulating resistance to insecticides. 38,[46][47][48][49] The AKH titer in hemolymph of the locust Schistocerca gregaria increased post-treatment with deltamethrin.…”

Section: Introductionmentioning

confidence: 99%

Adipokinetic hormone signaling regulates cytochrome P450‐mediated chlorantraniliprole sensitivity in Spodoptera frugiperda (Lepidoptera: Noctuidae)

Tan

Chen

et al. 2022

Pest Management Science

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BACKGROUND: Fall armyworm (FAW, Spodoptera frugiperda) is one of the most destructive and invasive pests worldwide and causes significant economic losses. Intensive and frequent use of insecticides has led to the development of resistance in FAW. Adipokinetic hormone (AKH) have been proven to be involved in insecticide resistance in insects. However, the molecular mechanism underlying chlorantraniliprole resistance mediated by AKH signaling in FAW remains unclear.RESULTS: The expression of SpfAKHR was highest in male adults and lowest in 1st instar larvae. SpfAKH was expressed the highest in eggs and the lowest in 6th instar larvae. AKH signaling was involved in the sensitivity of FAW to chlorantraniliprole through a toxicological bioassay, and the combination of chlorantraniliprole and bithionol (an inhibitor of key enzymes in the AKH pathway) significantly increased the mortality of FAW. Chlorantraniliprole significantly induced the expression of ten P450s, SpfAKH and SpfAKHR in FAW. RNA interference against SpfAKHR significantly decreased the P450 content, downregulated the expression of three P450 genes (SpfCYP6B50, SpfCYP321A9 and SpfCYP9A58) and inhibited the resistance of FAW to chlorantraniliprole. The topical application of AKH peptide significantly increased the P450 content, upregulated the expression of five P450 genes (SpfCYP321A9, SpfCY321A8, SpfCYP321A10, SpfCYP321A7 and SpfCYP6AB12), and enhanced the survival of FAW against chlorantraniliprole.CONCLUSIONS: AKH plays an important role in enhancing chlorantraniliprole resistance in FAW by exerting a positive influence on P450 gene expression and P450 content. These results provide valuable insights into insecticide resistance regulation and FAW control strategies.

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“…A previous study demonstrated that higher bombyxin signaling was detected in HCl-treated eggs compared to diapause eggs (Gu et al, 2019). Future that plays an important role in regulating trehalose homeostasis (Kikawada et al, 2007;Leyria et al, 2021). Transcriptional regulation of theTret gene by the juvenile hormone was reported in T.…”

Section: Changes In Gene Expressions and Ecdysteroid Levels In Dechor...mentioning

confidence: 99%

“…Facilitation of trehalose transporter 1 (Tret1), a conserved transporter in insects, plays a role in maintaining hemolymph trehalose levels in insects (Kanamori et al, 2010; Kikawada et al, 2007). Insulin signaling appears to positively affect Tret1 transcript expression in the ovaries of fed Rhodnius , a state where anabolic processes are required for egg formation and where it is known that an insulin signaling pathway is activated (Leyria et al, 2021). In Drosophila , stage‐dependent upregulation of Tret1 expression by ecdysteroid signaling was reported (Nishimura, 2020).…”

Section: Introductionmentioning

confidence: 99%

Expressions of sugar transporter genes during Bombyx mori embryonic development

Lin

Chang

2023

J Exp Zool Pt A

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Sugar transporters (Sts) play important roles in controlling carbohydrate transport and are responsible for mediating the movement of sugars into cells. Few studies have been conducted on expressions of Sts during insect embryonic development. In the present study, we investigated temporal expressions of St genes during the embryonic diapause process in Bombyx mori. We found that in HCl‐treated developing eggs, high gene expressions of trehalose transporter 1 (Tret1) were detected during middle and later embryonic development. St4 and St3 gene expressions gradually increased during the early stages, reached a small peak on Day 3, and large peaks were again detected on Day 7. However, in diapause eggs, expression levels of the Tret1, St4, and St3 genes all remained at low levels. Differential temporal changes in expressions of the Tret1, St4, and St3 genes found between diapause and HCl‐treated eggs were further confirmed using nondiapause eggs. Our results showed that nondiapause eggs exhibited similar changing patterns as those of HCl‐treated eggs, thus clearly indicating potential correlations between expressions of these genes and embryonic development. In addition, high gene expressions of Tret1 were also detected when dechorionated eggs were incubated in the medium. The addition of LY294002 (a specific phosphatidylinositol 3‐kinase [PI3K] inhibitor) and U0126 (a mitogen‐activated protein kinase/extracellular signal‐regulated kinase [ERK] kinase [MEK] inhibitor) partially inhibited Tret1 gene expression in dechorionated eggs, but did not affect either ecdysteroid‐phosphate phosphatase gene expression or ecdysteroid biosynthesis, clearly indicating that both PI3K and ERK are involved in increased gene expression of Tret1 that was independent of ecdysteroid levels. To our knowledge, this is the first comprehensive report to demonstrate the transcriptional regulation of St genes during embryonic development, thus providing useful information for a clearer understanding of insect egg diapause mechanisms.

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Regulation of a Trehalose-Specific Facilitated Transporter (TRET) by Insulin and Adipokinetic Hormone in Rhodnius prolixus, a Vector of Chagas Disease

Cited by 24 publications

References 94 publications

Insulin-Like Peptides and Cross-Talk With Other Factors in the Regulation of Insect Metabolism

Insulin-Like Peptides and Cross-Talk With Other Factors in the Regulation of Insect Metabolism

Adipokinetic hormone signaling regulates cytochrome P450‐mediated chlorantraniliprole sensitivity in Spodoptera frugiperda (Lepidoptera: Noctuidae)

Expressions of sugar transporter genes during Bombyx mori embryonic development

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