Regulation of activating protein-4-associated metastases of non-small cell lung cancer cells by miR-144

Gao, Feng; Wang, Tao; Zhang, Zefeng; Wang, Rui; Guo, Yang; Liu, Junfeng

doi:10.1007/s13277-015-3866-4

Cited by 15 publications

(12 citation statements)

References 35 publications

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“…This miRNA is abundantly expressed in red blood cells43, but has recently been investigated in lung cancer biology4445, showing a functional expression in lung cells.…”

Section: Discussionmentioning

confidence: 99%

Pulmonary microRNA profiles identify involvement of Creb1 and Sec14l3 in bronchial epithelial changes in allergic asthma

Bartel

Alessandrini

et al. 2017

Sci Rep

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Asthma is highly prevalent, but current therapies cannot influence the chronic course of the disease. It is thus important to understand underlying early molecular events. In this study, we aimed to use microRNAs (miRNAs) - which are critical regulators of signaling cascades - to identify so far uncharacterized asthma pathogenesis pathways. Therefore, deregulation of miRNAs was assessed in whole lungs from mice with ovalbumin (OVA)-induced allergic airway inflammation (AAI). In silico predicted target genes were confirmed in reporter assays and in house-dust-mite (HDM) induced AAI and primary human bronchial epithelial cells (NHBE) cultured at the air-liquid interface. We identified and validated the transcription factor cAMP-responsive element binding protein (Creb1) and its transcriptional co-activators (Crtc1-3) as targets of miR-17, miR-144, and miR-21. Sec14-like 3 (Sec14l3) - a putative target of Creb1 - was down-regulated in both asthma models and in NHBE cells upon IL13 treatment, while it’s expression correlated with ciliated cell development and decreased along with increasing goblet cell metaplasia. Finally, we propose that Creb1/Crtc1-3 and Sec14l3 could be important for early responses of the bronchial epithelium to Th2-stimuli. This study shows that miRNA profiles can be used to identify novel targets that would be overlooked in mRNA based strategies.

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“…This miRNA is abundantly expressed in red blood cells43, but has recently been investigated in lung cancer biology4445, showing a functional expression in lung cells.…”

Section: Discussionmentioning

confidence: 99%

Pulmonary microRNA profiles identify involvement of Creb1 and Sec14l3 in bronchial epithelial changes in allergic asthma

Bartel

Alessandrini

et al. 2017

Sci Rep

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“…miR-144, originally identified as an anti-oncogene and located in chromosome 10, played a crucial role in tumor cell proliferation, apoptosis, invasion, and metastasis [ 45 ]. Genome-wide analysis of miRNA expression has revealed that the sole miR-144 was consistently up-regulated in the aging brain, which is usually accompanied by a continuous cognitive decline [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

MiR-144 promotes β-amyloid accumulation-induced cognitive impairments by targeting ADAM10 following traumatic brain injury

et al. 2017

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The dysregulation expression of microRNAs (miRNAs) including miR-144, has been widely documented in TBI. However, little is known about the potential roles of miR-144 in the pathogenesis of TBI. In this study, we investigated the potential effects of miR-144 on cognitive function in vivo and in vitro. The results indicated that inhibition of miR-144 conferred a better neurological outcome after TBI in vivo, as evidenced by reduced lesion volume, alleviated brain edema and increased mNSS, of particular importance, improved cognitive deficits. In vitro, miR-144 knockdown protected neuron against Glu-induced injury, by enhancing cell viability, suppressing LDH release and caspase-3 activity, and reducing cognitive-related proteins levels. However, overexpression of miR-144 in vivo and in vitro showed the opposite effects. To further explore the molecular mechanisms underlying miR-144-induced cognitive dysfunctions, we found a significant inverse correlation between miR-144 and ADAM10 expression. Moreover, the direct interaction between miR-144 and ADAM10 3’-UTR was identified by dual-luciferase reporter assay. Also, we found miR-144 negatively regulated ADAM10 protein expression. Additionally, ADAM10 could modulate β-amyloid formation involved in cognitive deficits. Notably, ADAM10 knockdown by siRNA apparently abrogated miR-144 inhibitor-mediated neuroprotection. Taken together, these findings demonstrated that elevated miR-144 promoted cognitive impairments induced by β-amyloid accumulation post-TBI through suppressing of ADAM10 expression.

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“…mir-144 overexpression inhibited ap4-mediated cell invasiveness, while mir-144 depletion increased ap4-mediated cell invasiveness in the human a549 Nsclc line. The levels of mir-144 and ap4 inversely correlated in patient specimens 180 . Tang et al detected mir-25 expression in a549 cells, Nsclc and non-cancerous tissues.…”

Section: Mirna Involved In Bone Metastasismentioning

confidence: 91%

“…177 ). recent studies have reported that activating protein-4 (ap4) is upregulated in Nsclc and predicts poor prognosis in such patients [178][179][180] . ap4 has been shown to regulate cancer metastasis in Nsclc via regulating mTor/ p21 and transforming the growth factor β (TGFβ) receptor signalling pathway to increase emT to augment cell invasiveness.…”

Section: Mirna Involved In Bone Metastasismentioning

confidence: 99%

The role of microRNA in metastatic processes of non-small cell lung carcinoma

Pastorkova¹,

Škarda²,

Andel³

2016

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Background. MicroRNAs are small non-coding one-stranded RNA molecules that play an important role in the posttranscriptional regulation of genes. Bioinformatic predictions indicate that each miRNA can regulate hundreds of target genes. MicroRNA expression can be associated with various cellular processes leading to the metastasis of malignant tumours including non-small cell lung carcinoma. This review summarizes current knowledge on the role of microRNAs in NSCLC metastasis to the brain and lymph nodes. Methods. A search of the NCBI/PubMed database for publications on expression levels and the mechanisms of microRNA action in NSCLC metastasis. Results and Conclusion. Dysregulation of microRNAs in NSCLC can be associated with brain and lymph node metastasis. There are differences in microRNA expression profiling between NSCLC with and without metastases but it is currently not possible to reliably predict the site of metastasis in NSCLC. Based on data from RNAmicroarrays, bioinformatics analysis is able to predict the target genes of highlighted microRNAs, providing us with complex information about cancer cell features such as enhanced proliferation, migration and invasion. Such microRNAs may then be knocked-down using siRNAs or substituted with miRNA mimics. RNA microarray profiling may thus be a useful tool to select up-or down-regulated microRNAs. A number of authors suggest that microRNAs could serve as biomarkers and therapeutic targets in the treatment of NSCLC metastasis.

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Regulation of activating protein-4-associated metastases of non-small cell lung cancer cells by miR-144

Cited by 15 publications

References 35 publications

Pulmonary microRNA profiles identify involvement of Creb1 and Sec14l3 in bronchial epithelial changes in allergic asthma

Pulmonary microRNA profiles identify involvement of Creb1 and Sec14l3 in bronchial epithelial changes in allergic asthma

MiR-144 promotes β-amyloid accumulation-induced cognitive impairments by targeting ADAM10 following traumatic brain injury

The role of microRNA in metastatic processes of non-small cell lung carcinoma

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