Regulation of Allergic Reaction by Aerobic <i>Corynebacterium equi </i>Extract, CEF

Abstract

The effect of a water-soluble fraction (CEF) that was prepared from an extract of Corynebacterium equi on primary reaginic antibody formation was studied in Balb/c mice. Mice were immunized with a hapten carrier (DNP-OVA) and received intra-peritoneal injections of CEF 7 and 2 days prior to, or 2 and 7 days after the immunization. PCA titers of both antihapten (DNP) and anticarrier (OVA) antibodies of IgE class were reduced significantly by the CEF treatment. Evidence was presented in adoptive transfer experiments that the number of IgE-producing cells in the CEF-treated mice was lower than that of controls. Suppression of IgG1 anti-DNP antibody formation was also achieved by the CEF treatment. Formation of IgG1 anti-OVA antibodies, however, was not suppressed significantly by the treatment. The suppressive activities of CEF were shown to be dose-dependent, but timing of CEF administration did not appear critical.

Caracterização E Autenticação De Bactérias Gram-Positivas Armazenadas No Núcleo De Coleções De Micro-Organismos Do Instituto Adolfo Lutz

“…There is no evidence that such an event occurs in foals, though it seems likely that components of R. equi may induce immunosuppression of some type, as, for example, does the mycobacterial cell wall constituent, arabinomannan (65). Support for immunosuppressive activity of R. equi components comes from a study in which injection of mice with a water-soluble extract of R. equi suppressed production of reaginic antibodies against a hapten carrier (55).…”