Regulation of antibiotic resistance in Staphylococcus aureus

McCallum, Nadine; Berger‐Bächi, Brigitte; Senn, Maria M.

doi:10.1016/j.ijmm.2009.08.015

Cited by 85 publications

(60 citation statements)

References 158 publications

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“…Among the S. aureus isolates studied, various strains were found to be resistant to oxacillin and cefoxitin with disc diffusion tests but none of the isolates contained mecA gene by multiplex PCR. These results indicate that isolates may be a heteroresistant phenotype or carry amino acid substitutions in the transpeptidase domain of PBP2 which are responsible for the increased resistance (19,22).…”

Section: Discussionmentioning

confidence: 93%

Isolation and characterisation of Staphylococcus aureus strains isolated from beef, sheep and chicken meat

KEYVAN¹

2016

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The objective of this study was to evaluate the occurrence, enterotoxigenic properties and antibiotic resistance of Staphylococcus aureus in beef, sheep and chicken meat. For this purpose, 225 meat samples were obtained from different supermarkets in Ankara. Firstly, these samples were analysed phenotypically for coagulase-positive staphylococci and S. aureus. Then phenotypically determined S. aureus isolates were confirmed through PCR assay and investigated by multiplex PCR for enterotoxin genes and mecA gene. According to the analyses, 14.6%, 30.6% and 45.3% of the beef, sheep and chicken meat samples respectively were found to be contaminated with S. aureus. Eighty eight (77.1%) out of 114 S. aureus isolates were determined as enterotoxigenic. Also, isolates contained newly described enterotoxin like genes. These results indicated that new types enterotoxin genes could be effective in establishing food intoxication. In this study, isolates were found resistant to one or more antibiotics. None of the S. aureus isolates contain mecA gene but some of them were identified as resistant to a group of antibiotics like oxacillin and cefoxitin, which are used in determining methicillin resistance. For public health and hygienic meat production HACCP and GMP systems should be implemented effectively.Keywords: Enterotoxin, meat, mecA, PCR, Staphylococcus aureus. Sığır, koyun ve piliç etlerinden Staphylococcus aureus'un izolasyon ve karakterizasyonuÖzet: Bu çalışma sığır, koyun ve piliç etlerinde Staphylococcus aureus'un bulunuşu, enterotoksijenik özellikleri ve antibiyotik dirençliliklerini saptamak amacıyla yapılmıştır. Bu amaçla, Ankara'daki farklı süpermarketlerden 225 adet et örneği alınmıştır. Alınan örnekler öncelikle koagulaz-pozitif stafilokoklar ve S. aureus yönünden analiz edilmiştir. Fenotipik testler sonucu S. aureus olarak belirlenen suşlar PCR tekniği ile doğrulanarak, multipleks PCR tekniği kullanılarak enterotoksin genleri ve mecA geni yönünden analiz edilmiştir. Analiz sonuçlarına göre sığır, koyun ve piliç eti örnekleri S. aureus ile sırasıyla % 14.6, % 30.6 ve % 45.3 düzeyinde kontamine bulunmuştur. İzole edilen 114 adet S. aureus izolatından 88'inin (% 77.1) enterotoksijenik özellikte olduğu tespit edilmiştir. İzolatların yeni tanımlanan enterotoksin genlerine sahip oldukları belirlenmiştir. Sonuçlar, yeni tanımlanan enterotoksin genlerinin gıda intoksikasyonlarında önemli olabileceğini ortaya koymaktadır. Bu çalışmada izolatlar bir veya daha fazla antibiyotiğe dirençli bulunmuştur. S. aureus izolatlarının hiçbirinde mecA geni saptanmamasına karşın, bazı izolatlar metisilin duyarlılığının belirlenmesinde kullanılan, oxacillin ve cefoxitine dirençli bulunmuştur. Halk sağlığının korunması ve hijyenik et üretimi için HACCP ve GMP sistemlerinin etkin uygulanması önemlidir.

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Section: Discussionmentioning

confidence: 93%

Isolation and characterisation of Staphylococcus aureus strains isolated from beef, sheep and chicken meat

KEYVAN¹

2016

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…Modification of cell envelope represents an important mechanism against stress in various microbes (62)(63)(64). In the ⌬sll0794 mutant grown under ethanol stress, three proteins involved in cell envelope, Slr0298 of a FraH protein homolog, Sll0016 of a probable membrane-bound lytic transglycosylase A, and Sll0721 of an S-layer-RTX protein-related, were upregulated (Table III).…”

Section: Fig 3 Reproducibility Between Biological Replicatesmentioning

confidence: 99%

A Transcriptional Regulator Sll0794 Regulates Tolerance to Biofuel Ethanol in Photosynthetic Synechocystis sp. PCC 6803

Song

Chen

Wang

et al. 2014

Molecular & Cellular Proteomics

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To improve ethanol production directly from CO 2 in photosynthetic cyanobacterial systems, one key issue that needs to be addressed is the low ethanol tolerance of cyanobacterial cells. Our previous proteomic and transcriptomic analyses found that several regulatory proteins were up-regulated by exogenous ethanol in Synechocystis sp. PCC6803. In this study, through tolerance analysis of the gene disruption mutants of the up-regulated regulatory genes, we uncovered that one transcriptional regulator, Sll0794, was related directly to ethanol tolerance in Synechocystis. Using a quantitative iTRAQ-LC-MS/MS proteomics approach coupled with quantitative real-time reverse transcription-PCR (RTqPCR), we further determined the possible regulatory network of Sll0794. The proteomic analysis showed that in the ⌬sll0794 mutant grown under ethanol stress a total of 54 and 87 unique proteins were down-and upregulated, respectively. In addition, electrophoretic mobility shift assays demonstrated that the Sll0794 transcriptional regulator was able to bind directly to the upstream regions of sll1514, slr1512, and slr1838, which encode a 16.6 kDa small heat shock protein, a putative sodium-dependent bicarbonate transporter and a carbon dioxide concentrating mechanism protein CcmK, respectively. The study provided a proteomic description of the putative ethanol-tolerance network regulated by the sll0794 gene, and revealed new insights on the ethanol-tolerance regulatory mechanism in Synechocystis. As the first regulatory protein discovered related to ethanol tolerance, the gene may serve as a valuable target for transcription machinery engineering to further improve ethanol tolerance in Synechocystis. All MS data have been deposited in the ProteomeXchange with identifier PXD001266

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“…The reported amount of this metal in human body is approximately 0.03 ppm [32]. The daily intake of Cr in normal human from different sources is approximately 15-200 μg, which may sometimes reach up to 1 mg [33]. Trivalent Cr is an essential trace element for humans, which along with insulin removes glucose from blood, and plays a vital role in fat metabolism.…”

Section: Heavy Metals Concentrationmentioning

confidence: 99%

Heavy Metals Contamination Through Industrial Effluent to Irrigation Water in Gadoon Amazai (Swabi) and Hayatabad (Peshawar) Pakistan

et al. 2013

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Industrial effluents mixed water used for irrigation in vegetable growing area was tested for its heavy metal contents using Atomic Absorption Spectrophotometry. The overall metal concentration in the studied samples was in the order of Fe>Pb>Cu>Mn>Co>Zn>Cr>Ni (p < 0.05). pH was in the range of 3.1-8.7 and electrical conductivity was 325 to 1515 ms/cm. As all values were found to be increasing towards pollution limits, industrial effluents need a continuous monitoring and proper management before their discharge in order to reduce the future hazards in reference to aquatic and human life.

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Regulation of antibiotic resistance in Staphylococcus aureus

Cited by 85 publications

References 158 publications

Isolation and characterisation of Staphylococcus aureus strains isolated from beef, sheep and chicken meat

Isolation and characterisation of Staphylococcus aureus strains isolated from beef, sheep and chicken meat

A Transcriptional Regulator Sll0794 Regulates Tolerance to Biofuel Ethanol in Photosynthetic Synechocystis sp. PCC 6803

Heavy Metals Contamination Through Industrial Effluent to Irrigation Water in Gadoon Amazai (Swabi) and Hayatabad (Peshawar) Pakistan

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