Regulation of apoptosis by viruses that infect insects

Irusta, Pablo M.; Lamos, E.; Galonek, Heidi L.; Maten, M. A. Vander; Boersma, M. C. H.; Chen, Y.-B.; Hardwick, J. Marie

doi:10.1007/978-3-7091-0572-6_15

Cited by 2 publications

(1 citation statement)

References 36 publications

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“…Accumulation of dsRNA or viral proteins that modulate RNAi or apoptosis at certain levels may facilitate this rebound. [66][67][68] Real-time RT-PCR appears to be a practical and reliable method that may provide new insights into virus-vector interactions during the extrinsic incubation period. We are in the process of repeating this study in lines of Ae.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of Dengue-2 Virus Rna During the Extrinsic Incubation Period in Individual Aedes Aegypti

Richardson

Molina-Cruz

Salazar

et al. 2006

The American Journal of Tropical Medicine and Hygiene

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Dengue virus-2 (DENV-2) RNA was quantified from the midgut and legs of individual Aedes aegypti at each of 14 days postinfectious blood meal (dpi) in a DENV-2 susceptible strain from Chetumal, Mexico. A SYBR Green I based strand-specific, quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed. The lower detection and quantitation limits were 20 and 200 copies per reaction, respectively. Amounts of positive and negative strand viral RNA strands were correlated. Numbers of plaque-forming units (PFU) were correlated with DENV-2 RNA copy number in both C6/36 cell cultures and mosquitoes. PFU were consistently lower than RNA copy number by 2-3 log(10). Midgut levels of DENV-2 RNA peaked 8 dpi and fluctuated erratically between 6 and 9 dpi. Copies of DENV-2 RNA varied significantly among infected mosquitoes at each time point. Quantitative real-time RT-PCR is a convenient and reliable method that provides new insights into virus-vector interactions.

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Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of Dengue-2 Virus Rna During the Extrinsic Incubation Period in Individual Aedes Aegypti

Richardson

Molina-Cruz

Salazar

et al. 2006

The American Journal of Tropical Medicine and Hygiene

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Chikungunya Virus Induces a More Moderate Cytopathic Effect in Mosquito Cells than in Mammalian Cells

Siripanyaphinyo²,

Tumkosit³

et al. 2012

Intervirology

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Objectives: Chikungunya virus (CHIKV) is an alphavirus belonging to the Togaviridae family. Alphaviruses cause a chronic non-cytopathic infection in mosquito cells, while they develop a highly cytopathic infection in cells originating from various vertebrates. In this study, we compared the cytopathic effect (CPE) induced by CHIKV in Vero cells and a mosquito cell line, C6/36 cells. Methods: CPE and the virus titers were compared between the CHIKV-infected C6/36 and Vero cells. Apoptosis was measured by TUNEL assay, and the differences between the C6/36 and Vero cells were compared. Results: CHIKV infection induced strong CPE and apoptosis in the Vero cells, but light CPE in the C6/36 cells. The virus titers produced in the C6/36 cells were much higher than those produced in the Vero cells. Conclusions: The reason CHIKV induced strong CPE is that this virus triggers strong apoptosis in Vero cells compared with C6/36 cells. CHIKV established a persistent infection in C6/36 cells after being passaged 20 times. CHIKV infection in mosquito cells was distinct from that in Vero cells. The cell and species specificity of CHIKV-induced cell death implies that the cellular and viral regulators involved in apoptosis may play an important role in determining the outcome of CHIKV infection.

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Regulation of apoptosis by viruses that infect insects

Cited by 2 publications

References 36 publications

Quantitative Analysis of Dengue-2 Virus Rna During the Extrinsic Incubation Period in Individual Aedes Aegypti

Quantitative Analysis of Dengue-2 Virus Rna During the Extrinsic Incubation Period in Individual Aedes Aegypti

Chikungunya Virus Induces a More Moderate Cytopathic Effect in Mosquito Cells than in Mammalian Cells

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