Regulation of aromatase cytochrome P-450 (estrogen synthetase) transcripts in the quail brain by testosterone

Harada, Nobuhiro; Yamada, Kazuyo; Foidart, Agnès; Balthazart, Jacques

doi:10.1016/0169-328x(92)90146-3

Cited by 94 publications

(56 citation statements)

References 28 publications

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“…These observations imply that locally produced estrogens may influence sensory processing at the level of the spinal dorsal horn and may explain how gonadal hormones in males exert their pain-modulating function. Furthermore, Evrard and collaborators found that, in the spinal cord, aromatase expression is not controlled by testosterone, contrary to what is the case in many other parts of the central nervous system (Harada et al, 1992;Roselli et al, 1998), opening the possibility that, instead, neural stimuli may regulate its activity. It has been shown in cultured diencephalic neurons from fetal mice that several peptide neurotransmitters, such as substance P, and ␣-adrenergic agonists increase aromatase expression through the activation of protein kinases (Abe-Dohmae et al, 1996), and recent work suggests that activation of glutamate receptors inhibits aromatase activity in hypothalamic neurons by calcium-dependent phosphorylation (Balthazart et al, 1999).…”

mentioning

confidence: 85%

Sex hormones and pain: A new role for brain aromatase?

Blomqvist

2000

J. Comp. Neurol.

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mentioning

confidence: 85%

Sex hormones and pain: A new role for brain aromatase?

Blomqvist

2000

J. Comp. Neurol.

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“…It is generally accepted that the aromatase enzyme depends on the androgenic status of animals. Thus, castration decreases aromatase activity and mRNA levels, whereas T treatment restores them (Harada et al 1992, Abdelgadir et al 1994. Cytochrome P450 aromatase has been found in neurons from the cerebral cortex of neonatal rats (Zwain & Yen 1999).…”

Section: Discussionmentioning

confidence: 99%

Steroid 5α-reductase isozymes in the adult female rat brain: central role of dihydrotestosterone

Torres

Ortega

2006

Journal of Molecular Endocrinology

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The enzyme 5 -reductase (5 -R) (EC 1·3·99·5) exists as two isoforms, 5 -R type 1 (5 -R1) and 5 -R type 2 (5 -R2). 5 -R1 has been associated with catabolic functions whereas 5 -R2 has been associated with sexually dimorphic functions of the male. We recently demonstrated that both 5 -R isozymes are present in the central nervous system (CNS) of the adult male rat and are regulated in an opposing way by androgens. This finding raises the question as to whether both isozymes play a role in the sexual dimorphism of the CNS, besides other functions. To test this hypothesis, it is essential to study the regulation of both isozymes by androgens in the female. In this work, we studied the effects of testosterone (T) and dihydrotestosterone (DHT) on mRNA levels of both 5 -R isoforms in the prefrontal cortex of the adult female rat by one-step quantitative RT-PCR coupled with laser-induced fluorescence capillary electrophoresis. Our results demonstrate for the first time that 5 -R2 mRNA is slightly regulated by T and DHT in females. Surprisingly, 5 -R1 mRNA is not regulated by T in the intact female, whereas it is very positively regulated by DHT, a more potent androgen than T. These data indicate the great sexual dimorphism in the CNS with respect to both 5 -R isozymes, and suggest a crucial role of DHT in the sexual dimorphism of the CNS in the female. These results open up a new research line that may lead to a better understanding of the physiology of the CNS.

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“…AR are colocalized in aromatase-positive cells but no estrogen receptor-α (ERα) can usually be detected in most of the cells expressing this enzyme in the POM or BST, although ERα are present in many adjacent cells of the same nuclei. It has been hypothesized that the effects of estrogens on aromatase transcription are mediated transynaptically [79]while androgens may act directly on the aromatase gene [80, 81, 82, 83]. Studies undertaken with in vitro systems have shown that the interactions of SRC-1 with ERα are stronger than with AR but the relative importance of this coactivator for the modulation of both estrogen and androgen receptor action in vivo remains to be determined.…”

Section: Discussionmentioning

confidence: 99%

Steroid Receptor Coactivator SRC-1 Exhibits High Expression in Steroid-Sensitive Brain Areas Regulating Reproductive Behaviors in the Quail Brain

et al. 2002

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The steroid receptor coactivator SRC-1 modulates ligand-dependent transactivation of several nuclear receptors, including the receptors for sex steroid hormones. Reducing the expression of SRC-1 by injection of specific antisense oligonucleotides markedly inhibits the effects of estrogens of the sexual differentiation of brain and behavior in rats and inhibits the activation of female sexual behavior in adult female rats. SRC-1 thus appears to be involved in both the development and activation of sexual behavior. In the Japanese quail brain, we amplified by RT-PCR a 3,411-bp fragment extending from the HLH domain to the activating domain-2 of the protein. The quail SRC-1 is closely related to the mammalian (m) SRC-1 and contains a high proportion of GC nucleotides (62.5%). Its amino acid sequence presents 70% identity with mammalian SRC-1 and contains the three conserved LXXLL boxes involved in the interaction with nuclear receptors. In both males and females, RT-PCR demonstrates a similarly high level of expression in the telencephalon, diencephalon, optic lobes, brain stem, spinal cord, pituitary, liver, kidney, adrenal gland, heart, lung, gonads and gonoducts. Males express significantly higher levels of SRC-1 in the preoptic area-hypothalamus than females. In both sexes, lower levels of expression are observed in the cerebellum and muscles. In situhybridization utilizing a mixture of four digoxigenin-labeled oligonucleotides confirms at the cellular level the widespread distribution of SRC-1 mRNA in the brain and a particularly dense expression in steroid-sensitive areas that play a key role in the control of male sexual behavior. These data confirm the presence and describe for the first time the SRC-1 distribution in the brain of an avian species. They confirm its broad, nearly ubiquitous, distribution in the entire body including the brain as could be expected for a coactivator that regulates to the action of many nuclear receptors. However this distribution is heterogeneous in the brain and sexually differentiated in at least some areas. The very dense expression of SRC-1 in limbic and mesencephalic nuclei that are associated with the control of male sexual behavior is consistent with the notion that this coactivator plays a significant role in the activation of this behavior.

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Regulation of aromatase cytochrome P-450 (estrogen synthetase) transcripts in the quail brain by testosterone

Cited by 94 publications

References 28 publications

Sex hormones and pain: A new role for brain aromatase?

Sex hormones and pain: A new role for brain aromatase?

Steroid 5α-reductase isozymes in the adult female rat brain: central role of dihydrotestosterone

Steroid Receptor Coactivator SRC-1 Exhibits High Expression in Steroid-Sensitive Brain Areas Regulating Reproductive Behaviors in the Quail Brain

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