Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress

Hasanuzzaman, Mirza; Bhuyan, M. H. M. Borhannuddin; Anee, Taufika Islam; Parvin, Khursheda; Nahar, Kamrun; Mahmud, Jubayer Al; Fujita, Masayuki

doi:10.3390/antiox8090384

Cited by 815 publications

(523 citation statements)

References 254 publications

(298 reference statements)

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“…Antioxidant enzymes play an important role in ROS scavenging [9]. Since we found a significant reduction in ROS accumulation following exogenous Si treatment, we measured the activity of key antioxidant enzymes such as SOD, POD, and CAT in tomato leaves and roots under different treatments.…”

Section: Exogenous Silicon Alleviated Low Phosphorus-induced Oxidativmentioning

confidence: 99%

“…ROS overload results in the oxidation of lipids, proteins, amino acids, and nucleic acids [7,8]. To minimize ROS-induced damage to biomolecules, a plant should efficiently utilize its antioxidant defense system composed of enzymatic and nonenzymatic antioxidants [9]. The components of antioxidant defense system function collaboratively to keep the levels of ROS at optimal levels by a series of scavenging and/or detoxification processes.…”

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confidence: 99%

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Silicon Compensates Phosphorus Deficit-Induced Growth Inhibition by Improving Photosynthetic Capacity, Antioxidant Potential, and Nutrient Homeostasis in Tomato

et al. 2019

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Phosphorus (P) deficiency in soils is a major problem for sustainable crop production worldwide. Silicon (Si) is a beneficial element that can promote plant growth, development and responses to stresses. However, the effect of Si on tomato (Solanum lycopersicum L.) growth, photosynthesis and mineral uptake under P deficit conditions and underlying mechanisms remain unclear. Here, we showed that low P (LP) supply inhibited tomato growth as revealed by significantly decreased fresh and dry weights of shoots and impaired root morphological traits. LP-induced growth inhibition was associated with decreased photosynthetic pigment content, net photosynthetic rate (Pn), stomatal conductance, transpiration rate and water use efficiency. However, exogenous Si application alleviated LP-induced decreases in growth and physiological parameters. In particular, Si increased Pn by 65.2%, leading to a significantly increased biomass accumulation. Biochemical quantification and in situ visualization of reactive oxygen species (ROS) showed increased ROS (O2−· and H2O2) accumulation under LP stress, which eventually elevated lipid peroxidation. Interestingly, exogenous Si decreased ROS and malondialdehyde levels by substantially increasing the activity of antioxidant enzymes, including superoxide dismutase, peroxidase, and catalase. In addition, Si increased concentrations of osmoregulatory substances, such as proline, soluble sugar, soluble proteins, free amino acids, and organic acids under LP stress. Analysis of major element concentrations revealed that exogenous Si application under LP stress not only increased Si uptake but also enhanced the concentrations of most essential elements (K, Na, Ca, Mg, Fe, and Mn) in different tissues (roots, leaves, and stems). These results reveal that Si mitigates LP stress by improving photosynthetic capacity, antioxidant potential, and nutrient homeostasis and that it can be used for agronomic management of vegetable crops in P-deficient soils.

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Section: Exogenous Silicon Alleviated Low Phosphorus-induced Oxidativmentioning

confidence: 99%

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confidence: 99%

Silicon Compensates Phosphorus Deficit-Induced Growth Inhibition by Improving Photosynthetic Capacity, Antioxidant Potential, and Nutrient Homeostasis in Tomato

et al. 2019

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“…In plants, H2O2 is scavenged by CAT, POD, GPX, and APX into water [43,44]. Compare to APX, GPX, and POD, CAT shows low affinity to H2O2 with a high processing rate.…”

Section: Discussionmentioning

confidence: 99%

“…The Ascorbate-glutathione cycle is reported as the most important anti-oxidation metabolic pathway participates in of H2O2 metabolism and redox maintenance [44,56]. Enzyme like APX, GR, MDHAR and DHAR are very important in this cycle where APX reduces H2O2 to H2O through oxidation of ASA to protect plants from oxidative damage under abiotic stress.…”

Section: Discussionmentioning

confidence: 99%

Trehalose Protects Maize Plants from Salt Stress and Phosphorus Deficiency

Rohman¹,

Islam²,

Monsur³

et al. 2019

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This study was undertaken to elucidate the role of trehalose (Tre) in mitigating oxidative stress under salinity and low P in maize. Eight-day-old maize seedlings of two maize varieties, BARI Hybrid Maize-7 and BARI Hybrid Maize-9 were subjected to salinity (150 mM NaCl), low P (5 µM KH2PO4) and their combined stress with or without 10 mM Tre for 15-d.Salinity and combined stress significantly inhibited the shoot length, root length, and root volume, whereas, low P increased the root length and volume in both genotypes. Exogenous Tre in the stress treatments increased all of the growth parameters as well as decreased the salinity, low P and combined stress-mediated Na+/K+, ROS, MDA, LOX activity and MG in both genotypes. Under salinity and low P stress, the SOD activity increased in both genotypes, but the activity decreased in combined stress. POD activity increased in all stress treatments. Interestingly, Tre application enhanced the SOD activity in all the stress treatments but inhibited the POD activity. Both CAT and GPX activity were increased by saline and low P stress while the activities inhibited in combined stress. Similar results were found for APX, GR, and DHAR activities in both genotypes. However, MDHAR activity was inhibited in all the stresses. Interestingly, Tre enhanced CAT APX, GPX, GR, MDHAR and DHAR activities suggesting the amelioration of ROS scavenging in maize under all the stresses. Increased GST activity in presence or absence of Tre might involve in detoxification of hydroperoxides as well as leaf senescence. On the other hand, increased glyoxalase activities in saline and low P stress in BHM-9 suggested better MG detoxification system because of down-regulation of Gly-I activity in BHM-7 in those stresses. Tre also increased the glyoxalase activities in both genotypes under all the stresses. Tre improved the growth in maize seedlings by decreasing Na+/K+, ROS, MDA, and MG through regulating antioxidant and glyoxalase systems.

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“…Glutathione reductase reduces oxidized glutathione (GSSG) back into GSH to maintain balanced levels of the later in cells (Lu, 2013). Ascorbic acid scavenges ROS through glutathione-ascorbate pathway and serves as a cofactor for various enzymes (Hasanuzzaman et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Reduced Glutathione Protects Subcellular Compartments From Pb-Induced ROS Injury in Leaves and Roots of Upland Cotton (Gossypium hirsutum L.)

et al. 2020

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Heavy metals-based changes in the plants and their alleviation through eco-friendly agents including reduced glutathione (GSH) have been widely studied. In the present experiment, we tested the alleviatory role of reduced glutathione (GSH) in seedlings of upland cotton cultivar, TM-1 under lead (Pb) toxicity. Plants were grown in the Hoagland solution containing Pb (0 µM), Pb (500 µM), GSH (50 µM), and GSH + Pb (50 µM + 500 µM). Lead exposure exacerbated hydrogen peroxide (H 2 O 2 ) and hydroxyl radical (OH • ) levels, induced lipid peroxidation (MDA), and decreased the activities of catalase (CAT) and ascorbate peroxidase (APX) in the terminal and median leaves of 28-days old cotton seedlings stressed for 10 days. However, in the primary and secondary roots, CAT activity was increased but APX decreased. Similarly, peroxidase (POD) and superoxide dismutase (SOD) activities were enhanced in the median leaves but a declining trend was observed in the terminal leaves, primary roots and secondary roots. Glutathione reductase (GR) activity, ascorbic acid (AsA) contents and GSH concentrations were increased in all parts except AsA in the median leaves. Transmission electron micrographs of Pb-treated plants exhibited deformed cell wall and cell membrane, disfigured chloroplasts and irregularly shaped mitochondria in the terminal and median leaves. Further, cell membrane, mitochondria, nucleus and other cell organelles in root cells were severely affected by the Pb. Thus their identification was little bit difficult through ultramicroscopy. External GSH stabilized leaf and root ultramorphology by stabilizing cell membranes, stimulating formation of multivesicular body vesicles, and by maintaining structural integrity of other organelles. Evidently, GSH played major alleviatory role against Pb toxicity in upland cotton.

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Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress

Cited by 815 publications

References 254 publications

Silicon Compensates Phosphorus Deficit-Induced Growth Inhibition by Improving Photosynthetic Capacity, Antioxidant Potential, and Nutrient Homeostasis in Tomato

Silicon Compensates Phosphorus Deficit-Induced Growth Inhibition by Improving Photosynthetic Capacity, Antioxidant Potential, and Nutrient Homeostasis in Tomato

Trehalose Protects Maize Plants from Salt Stress and Phosphorus Deficiency

Reduced Glutathione Protects Subcellular Compartments From Pb-Induced ROS Injury in Leaves and Roots of Upland Cotton (Gossypium hirsutum L.)

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