Regulation of bacterial gene expression by non-coding RNA: It is all about time!

Chauvier, Adrien; Walter, Nils G.

doi:10.1016/j.chembiol.2023.12.011

Cited by 4 publications

(1 citation statement)

References 176 publications

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“…In recent years, a growing number of studies have uncovered new roles for non-coding RNAs in cellular regulatory processes in diverse organisms across all kingdoms of life, from bacteria to mammals (reviewed in [1][2][3][4][5][6]). These newly identified processes involve not only groups of non-coding RNAs that are assumed to have mainly regulatory functions, such as microRNA (miRNA) and miRNA-like molecules and long non-coding RNA (lncRNA), but also RNA molecules that have been known for decades to have primary functions in essential cellular processes, such as translation and mRNA splicing.…”

Section: Introductionmentioning

confidence: 99%

Non-Coding RNAs: Regulators of Stress, Ageing, and Developmental Decisions in Yeast?

Čáp,

Palková

2024

Cells

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Cells must change their properties in order to adapt to a constantly changing environment. Most of the cellular sensing and regulatory mechanisms described so far are based on proteins that serve as sensors, signal transducers, and effectors of signalling pathways, resulting in altered cell physiology. In recent years, however, remarkable examples of the critical role of non-coding RNAs in some of these regulatory pathways have been described in various organisms. In this review, we focus on all classes of non-coding RNAs that play regulatory roles during stress response, starvation, and ageing in different yeast species as well as in structured yeast populations. Such regulation can occur, for example, by modulating the amount and functional state of tRNAs, rRNAs, or snRNAs that are directly involved in the processes of translation and splicing. In addition, long non-coding RNAs and microRNA-like molecules are bona fide regulators of the expression of their target genes. Non-coding RNAs thus represent an additional level of cellular regulation that is gradually being uncovered.

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Section: Introductionmentioning

confidence: 99%

Non-Coding RNAs: Regulators of Stress, Ageing, and Developmental Decisions in Yeast?

Čáp,

Palková

2024

Cells

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Beyond ligand binding: Single molecule observation reveals how riboswitches integrate multiple signals to balance bacterial gene regulation

Chauvier,

Walter

2024

Current Opinion in Structural Biology

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A nascent riboswitch helix orchestrates robust transcriptional regulation through signal integration

Chauvier,

Dandpat,

Romero

et al. 2024

Nat Commun

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Widespread manganese-sensing transcriptional riboswitches effect the dependable gene regulation needed for bacterial manganese homeostasis in changing environments. Riboswitches – like most structured RNAs – are believed to fold co-transcriptionally, subject to both ligand binding and transcription events; yet how these processes are orchestrated for robust regulation is poorly understood. Through a combination of single-molecule and bulk approaches, we discover how a single Mn2+ ion and the transcribing RNA polymerase (RNAP), paused immediately downstream by a DNA template sequence, are coordinated by the bridging switch helix P1.1 in the representative Lactococcus lactis riboswitch. This coordination achieves a heretofore-overlooked semi-docked global conformation of the nascent RNA, P1.1 base pair stabilization, transcription factor NusA ejection, and RNAP pause extension, thereby enforcing transcription readthrough. Our work demonstrates how a central, adaptable RNA helix functions analogous to a molecular fulcrum of a first-class lever system to integrate disparate signals for finely balanced gene expression control.

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Regulation of bacterial gene expression by non-coding RNA: It is all about time!

Cited by 4 publications

References 176 publications

Non-Coding RNAs: Regulators of Stress, Ageing, and Developmental Decisions in Yeast?

Non-Coding RNAs: Regulators of Stress, Ageing, and Developmental Decisions in Yeast?

Beyond ligand binding: Single molecule observation reveals how riboswitches integrate multiple signals to balance bacterial gene regulation

A nascent riboswitch helix orchestrates robust transcriptional regulation through signal integration

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